Components of the four cell culture media used in the experiments. The table lists only the concentrations of additives used to supplement the basal media, except for antibiotics, where the manufacturer’s additives are presented in parentheses.
|
|
EBM |
HIMV |
MCDB |
MCDB-S |
|
|
Basal media |
EBM-2 Basal Medium (Lonza) |
AIM-V (Life Technologies) |
MCDB-131 (Life Technologies) |
MCDB-131 (Life Technologies) |
|
|
Originally suggested for |
Human microvascular endothelial cells |
Monocytes, Dendritic Cells, T Cells, Hybridomas, PBMCs, Fibroblasts, Macrophages, Myeloma Cells, Lymphocytes |
Human microvascular endothelial cells |
Human microvascular endothelial cells |
|
|
Additives |
Heat-inactivated fetal calf serum (FCS, PAN Biotech) |
5% |
1% |
5% |
1% |
|
Antibiotics |
30 μg/ml gentamicin and 15 ng/ml amphotericin |
(50 μg/ml streptomycin and 10 μg/ml gentamicin) |
100 U/ml penicillin and 100 μg/ml streptomycin |
100 U/ml penicillin and 100 μg/ml streptomycin |
|
|
Heparin |
– |
7.5 U/ml |
7.5 U/ml |
7.5 U/ml |
|
|
Chemically Defined Lipid Concentrate (Life Technologies) |
– |
– |
1% |
1% |
|
|
Glutamax (Life Technologies) |
– |
– |
1% |
1% |
|
|
Ascorbic acid |
75 μg/ml |
– |
5 μg/ml |
5 μg/ml |
|
|
Hydrocortisone |
0.2 μg/ml |
– |
250 nM |
250 nM |
|
|
HEPES |
– |
– |
10 mM |
10 mM |
|
|
Insulin Transferrin Selenium (Life Technologies) |
– |
– |
0,3% |
– |
|
|
Human recombinant epidermal growth factor (hrEGF, R&D Systems) |
10 ng/ml |
2 ng/ml |
2 ng/ml |
– |
|
|
Human recombinant basic fibroblast growth factor (hrBFGF) |
4 ng/ml |
1 ng/ml |
1 ng/ml |
– |
|
|
VEGF |
2 ng/ml |
– |
– |
– |
|
|
R3-IGF-1 |
5 ng/ml |
– |
– |
– |
|