Projects

Experimental methods

  • Measurement of the electrophysiological parameters of receptor pool and receptor kinetics of in vitro isolated organs.
  • Immunohistochemistry
  • In vivo antinociceptive tests, tolerance-dependence measurments, intracerebral, spinal application methods
  • Electrochemical HPLC measurement of transmitter release in isolated organs (rabbit ear artery, rabbit pulmonary artery, rat vas deferens, etc) and to detect specific enhancer effect in isolated brain regions (tuberculum olfactorium, substantia nigra, striatum, raphe, locus coeruleus)
  • Measuring transmitter release from in vitro tissue preparations (GABA, endomorphine 2, CGRP, [3H]-norepinephrine, dopamine, serotonin) by radioactive isotope and immunological technics
  • Measuring enhancer activity on cell cultures (PC12, HBEC) and stroke models
  • In vivo ulcer models
  • Measurement of gastrointestinal motility in vivo and in vitro
  • Acute and chronic inflammatory models
  • Measurement of microcirculation with Laser Doppler flowmetry and LASCA technique
  • Stereotaxic injection into given brain nuclei
  • Brain microdialysis in anesthetised and conscious rats. Measurements of biogenic amines and amino acids with HPLC and electrochemistry.
  • Measurements of radiolabelled neurotransmitter release from isolated brain slices and retina preparation using superfusion techniques.

Main cooperators

  • Department of Organic Chemistry, Semmelweis University
  • Department of Pharmacodynamics, Semmelweis University
  • Department of Pharmacology and Pharmacotherapy, University of Debrecen
  • Karl-Franzens University of Graz, Dept. of Experimental and Clinical Pharmacology, Graz, Austria
  • National Institute of Health and Medical Research (INSERM), Paris, France

Members