Measurement of the electrophysiological parameters of receptor pool and receptor kinetics of in vitro isolated organs.
In vivo antinociceptive tests, tolerance-dependence measurments, intracerebral, spinal application methods
Electrochemical HPLC measurement of transmitter release in isolated organs (rabbit ear artery, rabbit pulmonary artery, rat vas deferens, etc) and to detect specific enhancer effect in isolated brain regions (tuberculum olfactorium, substantia nigra, striatum, raphe, locus coeruleus)
Measuring transmitter release from in vitro tissue preparations (GABA, endomorphine 2, CGRP, [3H]-norepinephrine, dopamine, serotonin) by radioactive isotope and immunological technics
Measuring enhancer activity on cell cultures (PC12, HBEC) and stroke models
In vivo ulcer models
Measurement of gastrointestinal motility in vivo and in vitro
Acute and chronic inflammatory models
Measurement of microcirculation with Laser Doppler flowmetry
Stereotaxic injection into given brain nuclei
Genotyping of KO-mice with PCR
Patch clamp method
Brain microdialysis in anesthetised and conscious rats. Measurements of biogenic amines and amino acids with HPLC and electrochemistry.
Measurements of radiolabelled neurotransmitter release from isolated brain slices and retina preparation using superfusion techniques.