Our new spectral flow cytometer records the full spectrum of light emitted by labeled cells, unlike conventional instruments. This technique offers several advantages, including the ability to use very similar fluorescent dyes and efficient separation of autofluorescent signals from cells that interfere with measurements. Additionally, up to 20 targets can be examined simultaneously in a single tube with increased sensitivity.
During the training sessions, our colleagues learned the basics of system operation, the fluidics of the system, the spectral unmixing algorithm, autofluorescence extraction options, the design of experiments with multicolor immunophenotyping panels, and the use of the software.
Our growing toolbox will enable us to further enhance our research methods in cardio-immunology and oncopharmacology.