What are we studying?
The Haemostasis Division performs research in the field of atherothrombosis in attempt to characterize its molecular pathomechanism culminating in cardiovascular and cerebrovascular diseases, which continue to be a major public health concern worldwide. The work in the division focuses on the enzymological evaluation of proteases involved in the dissolution of thrombi. A second area of interest is the interactions of blood vessel wall components and blood cells, which contribute to the formation and stabilization of intravascular thrombi. A major goal of the research is the identification of molecular targets, which allow development of thrombolytic strategies with higher efficiency than the therapeutic approaches available at present. Applying an array of in vitro techniques (scanning electron microscopy, immunofluorescent confocal microscopy, rheometry, turbidimetry, surface plasmon resonance, microcalorimetry) we characterise the interactions of haemostatic factors, the structure and stability (mechanical, chemical) of isolated fibrin and plasma clots.
How do we research?
At the level of tissues, vascular wall:
- immunofluorescent microscopy, AFM, SEM
Cells and supramolecular assemblies:
- flow chamber, ELISA, AFM, SEM
- SPR, FRET, LSM, turbidimetric assay, colorimetric and fluorescence measurements
in silico modeling:
- stochastic and deterministic simulations, PDE, MCA
Who are we?
Since 1991 the teaching and research activities of Professor Krasimir Kolev have been linked to the Department of Biochemistry of Semmelweis University. His research achievements in the field of thrombosis and haemostasis have been widely recognized nationally and internationally (he was elected for two cycle as a president of the Hungarian Society on Thrombosis and Haemostasis, as a co-chair of the Fibrinolysis subcommittee of the International Society on Thrombosis and Haemostasis, and currently as a board member of the International Society for Fibrinolysis and Proteolysis). A hallmark of his fruitful research-team management is the success of the numerous PhD graduates mentored by him and now working as active researchers in his team or abroad.
Staff members and publications
- Kolev Krasimir, MD, PhD, DSc
- Erzsébet Komorowicz, MD, PhD
- Imre Varjú, MD, PhD
- Katalin Bartha, PhD
- Anna Tanka-Salamon, PhD
- László Beinrohr, PhD
- Veronika Judit Farkas, MD
- László Szabó
- Erzsébet Tóth Maróthyné
- Gurabi Anna
- Barnabás Egri
- Sándor Zsófia
- Györgyi Oravecz
- Krisztián Bálint
“HUNGARIAN CENTRE OF EXCELLENCE FOR MOLECULAR MEDICINE” workgroup
- Nikolett Wohner, PhD, HCEMM group leader
- Alexandra Raska, MD