Head of the Laboratory:
Dr. Béla Molnár, M.D., Ph.D., D. Sc.
Scientific advisor, Doctor of Science

RESEARCH

Molecular Gastroenterology Laboratory is the gastroenterological research laboratory of the Clinic. Its scientific research area includes the molecular basis of colorectal cancer development, genetic and epigenetic analysis of ulcerative colitis, relationship between gastric ulcer and Helicobacter pylori infection, molecular background of reflux disease, and further research projects in Hungarian and international cooperation.

The Laboratory analyses the tissue biopsy and peripheral blood specimens from the biobank constructed in cooperation with Endoscopic Laboratory of the Gastroenterology Department. The Laboratory has a comprehensive experience in analysis of DNA, RNA, microRNA, protein and long non-coding RNA (lncRNA) cell components. Specialty of the Laboratory is the combination of morphological and molecular biological analyses. Besides tissue suspension analysis, cell isolation techniques such as laser microdissection (LCM) are also available. Using LCM a selected cell population (e.g. epithelial, stromal, immune and tumor cells) can be separated for further molecular analysis. The Laboratory has introduced systemic, whole genomic analysis techniques such as microarrays for DNA methylation, long non-coding RNA, microRNA and mRNA expression analysis. The molecular biological results can be validated also on protein level using tissue microarray (TMA) technology.



RESEARCH TECHNIQUES, METHODS

Cell component isolation
DNA, long non-coding RNA,
microRNA, mRNA, protein
manual, automatic,
plasma cell-free DNA

Amplification
DNA whole genomic amplification
RNA

Array Techniques
mRNA
microRNA
long non-coding RNA
DNA methylation

DNA sequencing
next generation DNA sequencing
mutation analysis
bisulfite sequencing

PCR analysis
RNA, microRNA, lncRNA,
DNA mutation and
copy number analysis,
DNA methylation analysis
Quantitative real-time PCR
multiplex PCR
Digital PCR

Morphological analysis
Tissue microarray (TMA) application
Digital microscopy
Quantitative histology
Immunohistochemistry
Molecular in situ analysis
mRNA, microRNA,
long non-coding RNA, FISH

Bioinformatics
SPSS
R
NGS sequencing data evaluation

Cell culture and
animal model experiments


Molecular Gastroenterology Laboratory studies systemic diseases, therefore applies high-throughput array techniques for DNA, RNA, microRNA, long non-coding RNA and protein analysis.

EDUCATION

The Laboratory offers an opportunity for making Scientific Students’ Associations (so called TDK) work and degree thesis. PhD students can also participate in research of the laboratory as PhD fellowship holders and with predoctoral fellowship until PhD degree.

Within Semmelweis University Clinical Doctoral School, Molecular Gastroenterology Laboratory of the 2nd Department of Internal Medicine, Semmelweis University participates in the following research projects:

Clinical Medical Doctoral School

Head: Dr. Zsolt Tulassay, 2nd Department of Internal Medicine

2/4. Program: Gastroenterology, Program-leader: Dr. Zsolt Tulassay
Tutor: Dr. Béla Molnár

RESEARCH PROJECTS

1. Analysis of peripheral blood mRNA expression and DNA markers of colorectal cancer patients

Tutor: Dr. Béla Molnár

Predoctor researcher:
Barbara Kinga Barták
TDK student
Eszter Márkus

2. Gene level and epigenetic alterations of aging in diseases of the gastrointestinal tract

Tutor: Dr. Béla Molnár

Predoctor researcher:
Dr. Árpád V. Patai
3. Regenerative and pathological alterations of the colon in the development of inflammatory and cancerous diseases

Tutor: Dr. Béla Molnár

4. Identification of epigenetic alterations and related microRNA, long non-coding RNA molecule changes involved in the development of colorectal cancer
Tutor: Dr. Béla Molnár,
Dr. Orsolya Galamb,
Dr. Alexandra Kalmár
Predoctor researcher: Zsófia Brigitta Nagy
TDK student:
Eszter Márkus, Krisztina Szigeti

5. Exosoma and multivesicular alterations along the development of colon tumors
Tutor: Dr. Gábor Valcz

6. Molecular biological and epigenetic analysis of serrated adenomas
Tutor: Dr. Árpád V. Patai, Dr. Béla Molnár
TDK student: Ádám Nagy


Within national and international collaborations the Laboratory works on common tasks, the group tests a variety of devices and develops molecular biological protocols and techniques.

In the laboratory several members are working in the Molecular Medicine Research Unit, Hungarian Academy of Sciences founded by Prof. Dr. Zsolt Tulassay. The Working Group renews its tasks and objectives in every 5 years. The Molecular Gastroenterology Working Group ensures the resources required for the work and the financial background with Hungarian OTKA, industrial and R & D grants and partially with international grants.


MEMBERS OF THE LABORATORY

  • Dr. Béla Molnár scientific advisor, clinical specialist, head of the laboratory
  • Orsolya Udvardyné Dr. Galamb senior researcher
  • Dr. Alexandra Kalmár postdoctor researcher
  • Dr. Gábor Valcz senior researcherr
  • Dr. Barnabás Wichmann postdoctor researcher
  • Dr. Árpád V. Patai predoctor researcher
  • Dr. Andrea Schöller predoctor researcher
  • Barbara Kinga Barták predoctor researcher
  • Zsófia Brigitta Nagy predoctor researcher
  • Gabriella Kónyáné Farkas qualified assistants
  • Hajnalka Kovács specimen collector assistant

TDK students

  • Krisztina Szigeti
  • Eszter Márkus
  • Ádám Nagy

Contact:

Telephone: +36-1-266-0926,
ext.: 55620, 55621, 55622, 55960