Leader: Prof. László Kőhidai
About the group
Website of the research group:
http://chemotaxis.semmelweis.hu
About the group
Main research interests of the group:
Investigations of phylogeny and ontogeny of chemotactic signalling. Drug-targeting. Evaluation of cell adhesion and migration in real-time, impedance based systems. Mathematical modelling of chemotaxis and chemotaxis assays.
Unicellular and mammalian cell culture models are used to investigate the optimal composition of chemotactic ligands in complex experiments focused on detailed description of ‘Chemotaxome’. Assays of cell physiology and molecular genetics are applied to describe special receptor dynamics of chemotactic selection.
An intensive research is going on to improve theory and practice of „chemotactic drug targeting”, and its clinico-pharmacology. Libraries of new anti-tumor conjugates are designed and tested. Tuftsin based oligopeptides furnished with fMLF chemoattractant moieties and methotrexate drug and GnRH based conjugates are used to characterize their cell physiological effects (e.g. chemotaxis, cell adhesion, phagocytosis, proliferation) and signalling mechanisms (e.g. receptor inhibition, IP3K blocking) in leukemic THP-1 monocyte and Mono Mac 6 monocyte models.
Analysis of cell adhesion – as the introductory phase of chemotaxis – is carried out by state of art impedance based techniques (ECIS and xCELLigence). The Research Group is the reference laboratory of Applied BioPhysics (USA), ibidi (Germany) and Roche-Hungary in Hungary. The mathematical routine developed by the Group makes possible to evaluate quantitatively even the micro motion of cells. The new ECIS Theta equipment working on 12 frequency ranges and measuring ohmic resistance (R), impedance (Z) and capacitance (C) provides the possibility to distinguish the number of adherent cells as well as their barrier function. A special wound healing assay is available to monitor migration of cells. xCELLigence SP and DP techniques are ideal complements of the above mentioned facilities – they are dedicated techniques of real time monitoring of cell adhesion and real chemotaxis even in low density cultures. In the frame of these experiments the group has an intensive work on design of novel chips adaptable into the equipments mentioned above.
The most recent trend of our research is to describe the molecular and cell biological backgrounds of beneficial effects of light treatment, described in clinical fields for decades. The Research Group has designed novel equipment,Cell-LED and a software controlling the lighting routines.
Collaborations:
In Hungary:
- ELTE-MTA Peptidkémiai Kutatócsoport, Budapest
- MTA MFA KI MEMS Laboratórium, Budapest
- SE, Gyógyszerhatástani Intézet Budapest
- SE, Szerves Vegytani Intézet, Budapest
- SE, Gyógyszertani Intézet, Budapest
- SzTE, Szent-Györgyi Albert Klinikai Központ, Neurológiai Klinika, Szeged
- SE, Parodontologiai Klinika, Budapest
- SE, Oralbiológiai Intézet, Budapest
- SE Testnevelési és Sporttudományi Tanszék, Budapest
- SE, Kardiológiai Központ, Budapest
- PTE, Szívgyógyászati Klinika, Pécs
- Bay Zoltán Alkalmazott Kutatási Közhasznú Nonprofit Kft.
Abroad:
- Dept. of Mechanical Engineering & Mechanics, School of Biomedical Engineering, Science & Health Systems, Drexel University, Philadelphia, USA
- Department of Functional Bioscience, Gifu University School of Medicine, Gifu, JAPAN
- Institut für Analytische Chemie, Chemo- & Biosensorik, Universität Regensburg
- Johns Hopkins SOM, Department Pathology, Division Medical Microbiology, Baltimore, USA
- Department of Pharmacology and Toxicology, Institute of Biomedicine, Faculty of Medicine, University of Oulu, Oulu, Finland
- Department of Etology, Ecology and Evolution, University of Pisa, Pisa, Italy
- Laboratorio de Fisiología Molecular, Instituto de Medicina Experimental, Escuela Luis Razetti, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela
- Faculty of Dental Medicine, Dept. Of Biochemistry, Carol Davila University, Bucharest, Romania
- Laboratory of Analytical Chemistry and Biopolymer Structure Analysis, Dept. of Chemistry, University of Konstanz, Konstanz, Germany
Members
Members
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