Purpose
The purpose of the HCA Core Facility is to provide access to a high-content screening microscope, enabling automated imaging and analysis of large numbers of cells in a multi-well format for quantitative microscopy.
Instrument
The HCA Core Facility ensures the use of a Thermo Scientific™ CellInsight™ CX5 High Content Screening (HCS) Platform.
Camera: Photometrics™ X1 CCD high-resolution fluorescence camera
- High-resolution, thermoelectrically cooled to 0°C
- 54 micron pixel size for high resolution detail in image capture and large field of view 2,208 by 2,208 pixel array
Light source:
The CX5 platform uses high-output LED light and is configured for imaging in the UV range through near-IR range with the provided filter sets. The platform offers the following excitation/ emission capabilities with Invitrogen™ Alexa Fluor™ dyes:
- 386/440 (e.g., Alexa Fluor 350 dye)
- 485/521 (e.g., Alexa Fluor 488 dye)
- 560/607 (e.g., Alexa Fluor 594 dye)
- 650/694 (e.g., Alexa Fluor 647 dye)
- 740/810 (e.g., Alexa Fluor 750 dye)
Objectives: Olympus™ 10x (0.3 NA) and 20x (0.4 NA) objectives
Detection Method: Brightfield, Fluorescent
Format: The system is compatible with most optically clear, flat, 8-well chamber slides; 6-, 12-, 24-, 48-, 96-, 384-, and 1,536-well microplates.
Software: Thermo Scientific™ HCS Studio 5.0 Cell Analysis Software for image acquisition and data analysis.
Equipped with an Onstage Incubator:
It enables precise control of temperature, humidity, and CO2 for time-lapse imaging of live cells under both physiological and non-physiological conditions. Environmental settings and image acquisition parameters are all seamlessly integrated into the HCS Studio software creating a powerful, walk-away platform for live cell quantitative imaging.
Advantages of the Onstage Incubator:
- Fully integrated environmental chamber for live cell time-lapse imaging
- Precisely maintain physiological or non-physiological conditions
- Simple scheduling to define frequency of imaging over a time course
- Powerful kinetic and motility measurements that increase information gathered from each cell over time
Services
- Acquisition
- Plate setup
- Analysis protocol setup
- Image analysis
- Training: regular users can be trained for image acquisition.
All sample preparation should be done by the user.
Charges
The HCA Core Facility operates on a non-profit basis within the university. Our fees include the maintenance fees and the actual salary costs.
Our services are available to all internal and external academic customers. Semmelweis University users have priority access.
First consultation and planning meeting is always free, further consultation and designs are charged 4.000 Ft/hour.
Internal users: 15.000 Ft/hour with assistance
10.000 Ft/hour without assistance
External and industrial customers please contact us for pricing.
All prices include taxes and university overheads.
To access our services, please contact us by email or phone.
Contact
Ágnes Varga, PhD
Phone: +36 1 459 1500 / 60369
Mobile: +36 30 016 4787
E-mail: varga.agnes1@semmelweis.hu
0.106 laboratory room
D part, ground floor
Basic Medical Science Center
Institute of Translational Medicine
Semmelweis University
1094 Budapest, Tűzoltó street. 37–47