Organotypic Post-mortem Adult Retina Explant as a Platform for Comprehensive Investigation
Our retina model system utilizes eyes procured from adult multi-organ donors harvested prior to cardiac arrest. Following dissection, organotypic cultures are prepared and positioned on a membrane substrate. The cultures are maintained for prolonged durations of more than 200 days within a chemically defined serum-free medium, ensuring physiological relevance and viability over an extended investigative period.
We have established a robust system capable of maintaining numerous explants. To date, our research has involved donors ranging from 18 to 74 years old, enabling an in-depth exploration of retinal physiology.
Our methodology integrates innovative techniques to examine retinal functionality comprehensively.
Applications:
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Viral Vector Testing: Assessment of cell-type specificity, efficacy, and safety of viral vectors for gene therapy applications in high-quality adult human retina organotypic culture.
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Toxicology Testing: Assessment of short- and long-term cytotoxicity and effects of drugs and pharmacological agents directly on human retinal tissue.
We welcome collaboration opportunities to advance research and application within our robust retina model system.