Head of Core Facility
Karolina Pircs, PhD
Core Facility Address
Basic Medical Science Center
Institute of Translational Medicine
Semmelweis University
0.112 laboratory room
D part, ground floor
Tűzoltó street. 37–47.
1094 Budapest
Contact E-mail
to: Ágnes Varga, PhD
varga.agnes1@semmelweis.hu
cc: Karolina Pircs, PhD
pircs.karolina@semmelweis.hu
Number of staff
3
Quality Control
ISO 9001
What we offer
- Generation of lentiviral vectors (overexpression of cDNA, shRNA, miRNA, etc.)
- Design and cloning
- Production, titration and quality control (QC) of viral vectors
- Generation of CRISPR/CAS9 lentiviral vectors for gene modification, gene therapy CRISPR KO, CRISPRi (CRISPR interference), CRISPRa (CRISPR activation)
- Design and cloning
- Production, titration and quality control (QC) of viral vectors
Why work with us
- Unique service for academic researchers in the country
(only available at Our University)
equipmentS
- BSL-2 cell culturing laboratory with Biobase Class II A2 Biological Safety Cabinet
| 4 pcs. CCL-170B-8 CelCulture Incubator 170L IR Sensor CO2 Control ULPA, Moist Heat Decon 230VAC 50/60HZ incubators (with GASGUARD 3250) |
| SW-32TI Beckman&Coulter Rotor for Ultracentrifugation |
| 734-3333 EVE™ Plus automata cell counter |
| Bio-Rad CFX Opus 384 Real-Time PCR System |
| VWR Chemi Premium gel-documentation system |
| Nuer -86C Ultralow Freezer wit HC |
| Pipettig robot: 96 Channel Disposable Tip Head; ST Liquid handling pipetting head for use with Agilent Liquid Handling Station |
| NanoDrop OneC Spectrophotometer |
| Optima xpn-80 ultracentrifuge |
| Zeiss Axiovert 40 CFL Inverted Fluorescence Phase Contrast Microscope Pred Axio Vert.A1 |
| VWR Mega Star 600R refrigerated centrifuge |
| VWR Micro Star 30R refrigerated microcentrifuge |
References
- Gábor Juhász (Momentum-BRC)
- Lajos Kemény (HCEMM-SU)
- Ákos Zsembery (SU)
- Zoltán Benyó (SU)
- Zoltán Giricz (SU)