Head of Core Facility
Krisztina, LUDÁNYI

Core Facility Address
Department of Pharmaceutics
Hőgyes E. u. 7.
Budapest, Hungary
H-1092

Contact E-mail
Dávid, VIRÁG
virag.david@semmelweis.hu

Staff
4

Quality Control
ISO90001

What we offer

  • Protein extraction from biological samples
  • Protein purification
  • Sample preparation for mass spectrometry-based proteomic/glycoproteomic analysis
  • Estimation of protein purity and protein quantity in samples
  • Release and derivatization of N-oligosaccharides for glycomic analysis

Why work with us

  • Our experienced team has a strong backround in proteomics and several key equipment to prepare samples for the mass spectrometry-based qualitative and quantitative analysis of protein/glycoprotein/glycan samples.

Our equipment

  • Pharmacia FPLC system equipped with UV detector
  • Agilent 1100 HPLC system with DAD and fluorescent detector
  • Uvitec Cambridge Gel electrophoresis and documentation system
  • Labogene Vacuum ceoncentrator and liophylizator
  • Grant-bio PCH-3 Block thermostat
  • Centrifuges
  • Semi-preparative chromatography columns (size exclusion, ion-exchange, affinity)

References

  • DOI: 10.1007/s10337-019-03796-9
  • DOI: 10.3390/molecules26196003
  • DOI: 10.1016/j.btre.2021.e00637