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Medical Biotechnology MSc

Pázmány Péter Catholic University
Faculty of Information Technology and Bionics

Molecular biology subject
(6 credit points, terminal examination, Prof. Miklós Csala; P-ITOBA-0002)

Distance Teaching from the 23rd of March 2020
Lectures will continue online at the original timetable on Zoom platform (as video conferences). Invitation for each lecture will be emailed to the students registered to the subject. As Zoom video conference is interactive, students also have the opportunity to ask the lecturers live or written questions. Lecture slides continue to be available through this website.
Mid-term oral examinations will also be held on the Zoom interface (in the form of bilateral discussions) from the originally announced material, using the original questions and the originally planned evaluation and reward parameters.

Lecturers: Prof. Miklós Csala , Dr. Tamás Mészáros, Dr. Veronika Zámbó
Teaching secretary: Dr. Veronika Zámbó
2019/2020 Spring semester

Intro


The knowledge to be attained
The storage, maintenance and expression of genetic information, as well as their molecular mechanisms are fundamental topics of the course. DNA replication, DNA damage and repair, RNA synthesis and processing, protein synthesis, maturation and targeting, and different ways of regulation of gene expression are discussed. Regulation of cell cycle and apoptosis, in the light of the molecular background of tumor development is also part of the subject. Some of the lectures provide insights into molecular biology research.

Midterm tests and Students’ presentation
There are 3 evaluations during the semester.
The first two occasions (midterm tests) are oral examinations, at which a topic should be picked from the list and answered verbally. The third occasion is the Students’ presentation on selected articles. The student’s performance is evaluated from 1 to 5 in each of the three tests.
Students who miss a test due to illness (with a medical certificate presented) will be provided with a retake opportunity within one week.

Requirements for the signature
The course of the subject will not be recognized in case of more than 3 absences during the semester.

Examination
The semester ends with an oral terminal examination.
Students draw from two sets of topics, so the grade is based on two parts. The partial grades are offered in advance if marks of 3, 4 or 5 have been obtained at each of the three evaluations held during the term. Therefore, if the grade of the Students’ presentation is at least 3, then the grade of the first oral midterm (better than 2) is counted as the first, and that of the second oral midterm (better than 2) is counted as the second partial grade. Midterm marks worse than 3 do not yield such exemptions, and the student must draw a topic from the given set at the terminal exam. In case of a mark worse than 3 at the Students’ presentation, no partial grade is offered at the terminal exam, regardless of the performance at the midterm tests, i.e., the student has to answer a question from each set in the exam.

What to learn for the terminal examination?
– The material of all lectures during the semester.
– Medical Pathobiochemistry (eds. Mandl J and Machovich R) ISBN: 978 963 226 407 3
– Molecular Biology of the Cell (Alberts B, Johnson A, Lewis J, Raff M, Roberts K and Walter P) ISBN: 978-0815341055

Lectures


Location: ITK (1083 Bp., Práter utca 50/a.) room 320 – online from 23 March 2020
Duration: 2 x 45 min (Mondays 9:15-11:00 & Wednesdays 10:15-12:00)

 DateTimeLectureLecturer
110 February9:15Basics of molecular biologyCsala / Zámbó
212 February10:15DNA replicationZámbó
317 February9:15TranscriptionCsala
419 February10:15RNA processingCsala
524 February9:15TranslationMészáros
626 February10:15Post-translational modificationsMészáros
72 March9:15Intracellular protein trafficingMészáros
84 March10:15Control of translationMészáros
99 March9:15Control of gene expression at mRNA levelCsala
1011 March10:15DNA damage, mutation, repairZámbó
16 March--- Spring Break ---
18 March--- Spring Break ---
1123 March9:15Control of cell cycle I (online)Csala
1225 March10:15ORAL MIDTERM I (online)
1330 March 9:15Control of cell cycle II (online)Csala
141 April10:15Apoptosis I (online)Csala
6 April--- Spring Break ---
8 April--- Spring Break ---
13 April--- Heaving Monday ---
1515 April10:15Apoptosis II (online)Csala
1620 April9:15Molecular background of cancer I (online)Csala
1722 April10:15Molecular background of cancer II (online)Csala
1827 April9:15Molecular biology of viruses (online)Csala
1929 April10:15Protein degradation (proteasome and autophagy) (online)Mészáros
204 May9:15Methods of protein generation (online)Mészáros
216 May10:15Studies on protein-protein interactions (online)Mészáros
2211 May9:15ORAL MIDTERM II (online)
2313 May10:15Students' presentations and consultation (online)

Midterm 1


Topics for the 1st midterm

  1. Structure of DNA in prokaryotes and eukaryotes, histones, topoisomerism
  2. Proteins involved in prokaryotic DNA replication
  3. Initiation of replication and the replication fork
  4. Special features of eukaryotic DNA replication, the telomerase
  5. The most important types of DNA lesion, DNA damage and mutation
  6. Formation and repair of “mismatch damage”
  7. Ways to repair pyrimidine (Thymine) dimer
  8. Structure and function of E. coli RNA polymerase
  9. Initiation and termination of transcription in prokaryotic cells
  10. Initiation and termination of transcription in eukaryotic cells
  11. Processing of mRNA and the structure of mature mRNA
  12. Regulation of transcription in prokaryotes
  13. Regulation of transcription in eukaryotes
  14. Mechanisms for influencing eukaryotic gene expression (other than transcriptional control)
  15. Structure and function of RNAs involved in translation
  16. Mechanism of aminoacyl-tRNA formation
  17. Structure and function of prokaryotic and eukaryotic ribosomes
  18. Initiation of translation in prokaryotes and eukaryotes
  19. Elongation and termination of translation
  20. Post-translational modifications and functional consequences
  21. Eucaryotic cell organelles and the nuclear protein transport
  22. Protein transport to mitochondria and the ER
  23. Vesicular protein transport
  24. Possible mechanisms for the control of translation

Midterm 2


Topics for the 2nd midterm

  1. The eukaryotic cell division cycle – phases and checkpoints
  2. Cyclins, cyclin-dependent kinases and their inhibitors
  3. Control of cell cycle in G1 and S phases
  4. Control of cell cycle in G2 and M phases
  5. Handling DNA injuries and ongoing replication during the cell cycle
  6. Structure and function of apoptosome, DISC, and PIDDosome
  7. Types of Bcl-2 proteins and their role in apoptosis pathways of different origin
  8. Properties of caspases and their role in regulating apoptosis
  9. Components of the “survival signal” and its connection with the regulatory proteins of apoptosis
  10. The endoplasmic reticulum stress and the UPR
  11. Control of the level and activity of p53 protein
  12. Effects of p53 protein on cell cycle and apoptosis
  13. The concept of tumor suppressor genes, the functions of some representative tumor suppressor proteins
  14. Genetic modifications leading to tumor cell formation
  15. Grouping of animal viruses according to their replication mechanism, effect of double-stranded RNA in the eukaryotic cells
  16. Replication cycle of retroviruses, acutely transforming retroviruses
  17. Intracellular proteolysis, ubiquitination, proteasome
  18. Intracellular proteolysis, types and mechanisms of autophagy
  19. Protein production by using prokaryotic organisms
  20. Characteristics of eukaryotic and in vitro protein production systems
  21. The yeast two-hybrid system
  22. Fluorescence-based studies on protein-protein interactions

Download


Lecture slides are available here.

The passwords to download pages can be obtained from the laboratory teachers personally.