{"id":1615,"date":"2025-10-26T13:58:46","date_gmt":"2025-10-26T12:58:46","guid":{"rendered":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/?p=1615"},"modified":"2025-10-26T14:00:44","modified_gmt":"2025-10-26T13:00:44","slug":"new-preprint-is-live","status":"publish","type":"post","link":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/2025\/10\/26\/new-preprint-is-live\/","title":{"rendered":"New preprint is live!"},"content":{"rendered":"<p style=\"text-align: justify\" data-start=\"588\" data-end=\"788\">We are thrilled to share our latest study, now published on bioRxiv:<br data-start=\"660\" data-end=\"663\" \/><br \/>\n\ud83d\udc49 <a href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2025.10.24.684372v1\" target=\"_blank\" rel=\"noopener\">https:\/\/www.biorxiv.org\/content\/10.1101\/2025.10.24.684372v1<\/a><\/p>\n<p style=\"text-align: justify\" data-start=\"790\" data-end=\"1074\">In this work, we report the first phosphoproteomic analysis by mass spectrometry (P-MS) of human induced neurons (iNs) directly reprogrammed from Huntington\u2019s disease (HD) patient fibroblasts, integrated with matched proteomic and transcriptomic data from the same donors.<\/p>\n<p style=\"text-align: justify\" data-start=\"1076\" data-end=\"1700\">We identify 177 significantly altered phosphopeptides, revealing widespread signaling changes with enrichments in RNA splicing, autophagy, and stress-response pathways. Strikingly, we find binary phospho-switches (ON-OFF\/OFF-ON), including MXRA8, which shows a complete loss of phosphorylation at pS377 in HD-iNs despite increased MXRA8 protein abundance. Kinase analyses and co-IP experiments further implicate autophagy-linked regulators and reveal condition-specific MXRA8 interactors (e.g. LAMP1\/TGM2), supporting the role of altered phosphorylation in disrupted proteostasis in human HD neurons.<\/p>\n<p style=\"text-align: justify\" data-start=\"1702\" data-end=\"2110\">Led by our amazing shared first authors, Lea Danics and Chandramouli Muralidharan, whose dedication and teamwork drove this study, this work provides a new P-MS resource for the HD community. It represents a collaborative effort across Semmelweis University, HCEMM, and HUN-REN, together with international partners at Lund University, University of Cambridge, and University of Montreal.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>We are thrilled to share our latest study, now published on bioRxiv: \ud83d\udc49 <a href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2025.10.24.684372v1\" target=\"_blank\" rel=\"noopener\">https:\/\/www.biorxiv.org\/content\/10.1101\/2025.10.24.684372v1<\/a> In this work, we report the first phosphoproteomic analysis by mass spectrometry (P-MS) of human induced neurons (iNs) directly reprogrammed from Huntington\u2019s disease (HD) patient fibroblasts, integrated with matched proteomic and transcriptomic data from the same donors. We identify 177 significantly altered &hellip;<\/p>\n","protected":false},"author":101875,"featured_media":1301,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1615","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/wp-json\/wp\/v2\/posts\/1615","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/wp-json\/wp\/v2\/users\/101875"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/wp-json\/wp\/v2\/comments?post=1615"}],"version-history":[{"count":1,"href":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/wp-json\/wp\/v2\/posts\/1615\/revisions"}],"predecessor-version":[{"id":1616,"href":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/wp-json\/wp\/v2\/posts\/1615\/revisions\/1616"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/wp-json\/wp\/v2\/media\/1301"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/wp-json\/wp\/v2\/media?parent=1615"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/wp-json\/wp\/v2\/categories?post=1615"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/hcemm-neurobiology\/wp-json\/wp\/v2\/tags?post=1615"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}