{"id":48504,"date":"2014-11-09T14:01:16","date_gmt":"2014-11-09T13:01:16","guid":{"rendered":"http:\/\/semmelweis.hu\/hbtb\/microdis\/"},"modified":"2021-09-18T22:08:47","modified_gmt":"2021-09-18T20:08:47","slug":"microdissection","status":"publish","type":"page","link":"https:\/\/semmelweis.hu\/hbtb\/microdissection\/","title":{"rendered":"Microdissection"},"content":{"rendered":"<p align=\"justify\">The microdissection of human brain nuclei is based on a simple procedure, called the <strong>\u201cmicropunch technique\u201d<\/strong>.\u00a0This technique was introduced in 1973 <a href=\"https:\/\/semmelweis.hu\/hbtb\/files\/2015\/05\/PM118_BrainRes59_449.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><strong>Palkovits M:\u00a0Isolated removal of hypothalamic or other brain nuclei of the rat<\/strong>,<em> Brain Res<\/em> <strong>59<\/strong>: 449-450<\/a>\u00a0to remove small, precisely localized areas from a rat\u2019s brain.<\/p>\n<p align=\"justify\">Later, the technique was adapted for the microdissection of the human brain (Palkovits M, 1985, Neuromethods, Vol 1, eds: Boulton and Baker, Chapter 1, Humana Press, Clifton NJ, USA), and since 1992 the HBTB\u00a0has been collecting and storing thousands of microdissected human brain nuclei and areas.<\/p>\n<p align=\"justify\">Since 2012 the HBTB\u00a0is supported by the Semmelweis University.<\/p>\n<h3>Basic conditions for microdissections of brain samples<\/h3>\n<ol>\n<li>Standard technical conditions are required<\/li>\n<li>Information about the post mortem delay\n<ul type=\"disc\">\n<li>microdissection with the lowest post mortem delay<\/li>\n<li>control of the post mortem changes by using neurosurgical brain samples with \u201c0 hour\u201d post mortem delay<\/li>\n<\/ul>\n<\/li>\n<li>The optimal size (weight) of the brain samples should be decided before dissection (macro- or microdissection?)<\/li>\n<li>The optimal number of brain samples\n<ul type=\"disc\">\n<li>optimal number for statistical analysis<\/li>\n<li>optimal number to be representative for the whole brain<\/li>\n<\/ul>\n<\/li>\n<li>Storage of the dissected brain samples in proper conditions<\/li>\n<li>Basic neuroanatomical and histological knowledge about the brain samples (cellular homogeneity, neuronal density, gray\/white matter ratio, vascular density) to be dissected<\/li>\n<\/ol>\n<h3>Basic requirements for microdissections of human brain samples<\/h3>\n<p><strong>Basic information about:<\/strong><\/p>\n<ol start=\"7\">\n<li>the cause of death<\/li>\n<li>the condition of death (duration of the agony)<\/li>\n<li>any long-term disease (medical report)<\/li>\n<li>medical treatments before death (medical report)<\/li>\n<li>drug abuse, alcoholism, smoking, etc. (family report)<\/li>\n<li>pathological report<\/li>\n<li>neuropathological report<\/li>\n<\/ol>\n<p><strong>Special points that should be taken into account for dissecting and collecting brain samples appropriate to the research plan differences can be made by:<\/strong><\/p>\n<ol start=\"14\">\n<li>sex<\/li>\n<li>age<\/li>\n<li>lateralization of the brain<\/li>\n<\/ol>\n<p><strong>Microdissections of brain samples from persons with neurodegenerative or psychiatric disorders:<\/strong><\/p>\n<ol start=\"17\">\n<li>correct and well-documented neurological or psychiatric reports about the\n<ul type=\"disc\">\n<li>diagnosis<\/li>\n<li>duration of the disease<\/li>\n<li>long-term medical treatments<\/li>\n<\/ul>\n<\/li>\n<li>to investigate neurological or psychiatric disorders by using microdissection, appropriatecontrol microdissected samples are required (comparable brain samples from persons died without neurodegenerative or psychiatric disorders: sudden death, traffic accidents, etc.)<\/li>\n<\/ol>\n<h3 align=\"center\">\u00a0<\/h3>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The microdissection of human brain nuclei is based on a simple procedure, called the \u201cmicropunch technique\u201d.\u00a0This technique was introduced in 1973 <a href=\"https:\/\/semmelweis.hu\/hbtb\/files\/2015\/05\/PM118_BrainRes59_449.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Palkovits M:\u00a0Isolated removal of hypothalamic or other brain nuclei of the rat, Brain Res 59: 449-450<\/a> \u00a0to remove small, precisely localized areas from a rat\u2019s brain. Later, the technique was adapted for the microdissection of the human brain (Palkovits M, 1985, Neuromethods, Vol 1, eds: Boulton and Baker, Chapter 1, &hellip;<\/p>\n","protected":false},"author":12,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-fullwidth.php","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[],"class_list":["post-48504","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/pages\/48504","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/comments?post=48504"}],"version-history":[{"count":10,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/pages\/48504\/revisions"}],"predecessor-version":[{"id":49132,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/pages\/48504\/revisions\/49132"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/media?parent=48504"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/categories?post=48504"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/tags?post=48504"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}