{"id":48499,"date":"2014-11-09T14:01:16","date_gmt":"2014-11-09T13:01:16","guid":{"rendered":"http:\/\/semmelweis.hu\/hbtb\/protocol\/"},"modified":"2021-09-18T21:19:24","modified_gmt":"2021-09-18T19:19:24","slug":"protocol","status":"publish","type":"page","link":"https:\/\/semmelweis.hu\/hbtb\/introduction\/protocol\/","title":{"rendered":"Protocol"},"content":{"rendered":"<p align=\"justify\">Within a country-wide program (Lenhoss\u00e9k Program), the HBTB has been contracted with Hungarian pathological, neurological and neurosurgical departments or laboratories to collect human brains. <i>Post mortem delay: 2-6 h<\/i><\/p>\n<p align=\"justify\"><b>Permissions:<\/b><\/p>\n<ul type=\"disc\">\n<li>family consent or medicolegal cases<\/li>\n<\/ul>\n<p align=\"justify\"><b>Obligatory information:<\/b><\/p>\n<ul type=\"disc\">\n<li>family report<\/li>\n<li>medical report (died in public place) or<\/li>\n<li>clinical report (died in hospital)<\/li>\n<li>pathological report<\/li>\n<li>tests for: HIV, hepatitis, syphylis, Tb, drugs<\/li>\n<li>neuropathological report<\/li>\n<\/ul>\n<h3 style=\"margin-top: 60px\">Specification of the brain samples<\/h3>\n<p><i><b>Macrodissected brain areas<\/b><\/i> (in 2.0 ml Eppendorf tubes) <br \/>\n57 different brain regions (representative for the whole brain) <br \/>\n0.1-2.0\u00a0g tissue weight<\/p>\n<p><i><b>Microdisssected brain nuclei and areas<\/b><\/i> (in 1.5 ml Eppendorf tubes) 236\u00a0different brain regions 1.0-100 mg tissue weight<\/p>\n<p><i><b>Topographically oriented brain tissue blocks<\/b><\/i> <br \/>\n72\u00a0different brain regions in about 1.0 \u00a01.0\u00a0\u00d7 2.0 cm size ready for cryostat sectioning and in situ hybridization histochemistry<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Within a country-wide program (Lenhoss\u00e9k Program), the HBTB has been contracted with Hungarian pathological, neurological and neurosurgical departments or laboratories to collect human brains. Post mortem delay: 2-6 h Permissions: family consent or medicolegal cases Obligatory information: family report medical report (died in public place) or clinical report (died in hospital) pathological report tests for: &hellip;<\/p>\n","protected":false},"author":12,"featured_media":0,"parent":48495,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"template-fullwidth.php","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[],"class_list":["post-48499","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/pages\/48499","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/comments?post=48499"}],"version-history":[{"count":9,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/pages\/48499\/revisions"}],"predecessor-version":[{"id":49123,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/pages\/48499\/revisions\/49123"}],"up":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/pages\/48495"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/media?parent=48499"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/categories?post=48499"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/hbtb\/wp-json\/wp\/v2\/tags?post=48499"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}