{"id":588,"date":"2026-08-24T12:17:41","date_gmt":"2026-08-24T10:17:41","guid":{"rendered":"https:\/\/semmelweis.hu\/gsi\/?page_id=588"},"modified":"2026-08-24T17:11:53","modified_gmt":"2026-08-24T15:11:53","slug":"vekop-2-3-3-15-2016-00016","status":"publish","type":"page","link":"https:\/\/semmelweis.hu\/gsi\/rolunk\/palyazatok\/vekop-2-3-3-15-2016-00016\/","title":{"rendered":"VEKOP-2.3.3-15-2016-00016 (2017-2021)"},"content":{"rendered":"<h2><a href=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/infoblokk_kedv_final_felso_cmyk_ERFA_cut.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-597\" src=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/infoblokk_kedv_final_felso_cmyk_ERFA_cut-400x276.png\" alt=\"\" width=\"289\" height=\"200\" srcset=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/infoblokk_kedv_final_felso_cmyk_ERFA_cut-400x276.png 400w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/infoblokk_kedv_final_felso_cmyk_ERFA_cut-1024x708.png 1024w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/infoblokk_kedv_final_felso_cmyk_ERFA_cut-768x531.png 768w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/infoblokk_kedv_final_felso_cmyk_ERFA_cut-175x120.png 175w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/infoblokk_kedv_final_felso_cmyk_ERFA_cut-753x520.png 753w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/infoblokk_kedv_final_felso_cmyk_ERFA_cut.png 1453w\" sizes=\"auto, (max-width: 289px) 100vw, 289px\" \/><\/a> <a href=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/vekop-2.3.3_tabla.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-598\" src=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/vekop-2.3.3_tabla-400x283.png\" alt=\"\" width=\"282\" height=\"200\" srcset=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/vekop-2.3.3_tabla-400x283.png 400w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/vekop-2.3.3_tabla-1024x725.png 1024w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/vekop-2.3.3_tabla-768x544.png 768w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/vekop-2.3.3_tabla-753x533.png 753w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/vekop-2.3.3_tabla.png 1120w\" sizes=\"auto, (max-width: 282px) 100vw, 282px\" \/><\/a><\/h2>\n<h2>\u00a0<\/h2>\n<h2>\u00a0<\/h2>\n<h2>\u00a0<\/h2>\n<h2>\u00a0<\/h2>\n<h2>Magyar K\u00f6zponti Extracellul\u00e1ris Vezikula Kutat\u00f3, Szolg\u00e1ltat\u00f3 \u00e9s Referencia Laborat\u00f3rium l\u00e9trehoz\u00e1sa a Semmelweis Egyetemen<\/h2>\n<p><em>VEKOP-2.3.3-15-2016-00016 p\u00e1ly\u00e1zati t\u00e1mogat\u00e1ssal<\/em><\/p>\n<p><strong>A projekt id\u0151tartama:<\/strong> 2017. j\u00falius 1 &#8211; 2021. j\u00fanius 30.<\/p>\n<p><strong>A t\u00e1mogat\u00e1s \u00f6sszege:<\/strong> 299,65 milli\u00f3 Ft<\/p>\n<p><strong>A t\u00e1mogat\u00e1s m\u00e9rt\u00e9ke:<\/strong> 100%<\/p>\n<p><strong>Kedvezm\u00e9nyezett:<\/strong> Semmelweis Egyetem<\/p>\n<p><a href=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/VEKOP_dokumentacio_2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-591 size-medium alignright\" src=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/VEKOP_dokumentacio_2-299x400.jpg\" alt=\"\" width=\"299\" height=\"400\" srcset=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/VEKOP_dokumentacio_2-299x400.jpg 299w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/VEKOP_dokumentacio_2.jpg 590w\" sizes=\"auto, (max-width: 299px) 100vw, 299px\" \/><\/a> <a href=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/VEKOP_dokumentacio.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-590 size-medium alignright\" src=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/VEKOP_dokumentacio-400x400.jpg\" alt=\"\" width=\"400\" height=\"400\" srcset=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/VEKOP_dokumentacio-400x400.jpg 400w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/VEKOP_dokumentacio-150x150.jpg 150w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/VEKOP_dokumentacio.jpg 561w\" sizes=\"auto, (max-width: 400px) 100vw, 400px\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p><strong>A megval\u00f3s\u00edt\u00e1s sz\u00ednhelye:<\/strong> Semmelweis Egyetem, 1089 Budapest, Nagyv\u00e1rad t\u00e9r 4.<\/p>\n<ul>\n<li>Genetikai, Sejt \u00e9s Immunbiol\u00f3giai Int\u00e9zet (NET VI. emelet)<\/li>\n<li>Farmakol\u00f3giai \u00e9s Farmakoter\u00e1pi\u00e1s Int\u00e9zet (NET IV. emelet)<\/li>\n<\/ul>\n<div class=\"responsive-tabs\">\n<h2 class=\"tabtitle\">\u00d6sszefoglal\u00f3<\/h2>\n<div class=\"tabcontent\">\n\n<p>A projekt c\u00e9lja olyan Magyar K\u00f6zponti Extracellul\u00e1ris Vezikula Kutat\u00f3, Szolg\u00e1ltat\u00f3 \u00e9s Referencia Laborat\u00f3rium l\u00e9trehoz\u00e1sa volt a Semmelweis Egyetemen rendelkez\u00e9sre \u00e1ll\u00f3 extracellul\u00e1ris vezikula kutat\u00f3 b\u00e1zison, amely mind Magyarorsz\u00e1gon, mind K\u00f6z\u00e9p-Kelet Eur\u00f3p\u00e1ban egyed\u00fcl\u00e1ll\u00f3an, s\u0151t eur\u00f3pai \u00e9s vil\u00e1gviszonylatban is kiemelked\u0151 m\u00f3don lesz k\u00e9pes az EV-k m\u00e9r\u00e9s\u00e9re \u00e9s izol\u00e1l\u00e1s\u00e1ra nem csak alapkutat\u00e1s keret\u00e9ben, hanem ak\u00e1r rutinszer\u0171en, nagy \u00e1tereszt\u0151k\u00e9pess\u00e9ggel is.<\/p>\n<p>A VEKOP-2.3.3-15-2017-00016 p\u00e1ly\u00e1zat keret\u00e9ben, a legmagasabb nemzetk\u00f6zi elv\u00e1r\u00e1soknak megfelel\u0151 Extracellul\u00e1ris Vezikula Kutat\u00f3, Szolg\u00e1ltat\u00f3 \u00e9s Referencia Laborat\u00f3rium felszerel\u00e9s\u00e9hez sz\u00fcks\u00e9ges kulcsm\u0171szerek ker\u00fcltek beszerz\u00e9sre a Semmelweis Egyetem kutat\u00f3b\u00e1zis\u00e1n.<\/p>\n<p><strong>A M\u0171szerek:<\/strong><\/p>\n<ul>\n<li>preparat\u00edv ultracentrifuga (Beckman Coulter) az extracellul\u00e1ris vezikul\u00e1k szepar\u00e1l\u00e1s\u00e1hoz<\/li>\n<li>Particle Metrix gy\u00e1rtm\u00e1ny\u00fa Zetaview nanor\u00e9szecske sz\u00e1ml\u00e1l\u00f3 (NTA) berendez\u00e9s vezikulasz\u00e1m \u00e9s m\u00e9reteloszl\u00e1s meghat\u00e1roz\u00e1shoz<\/li>\n<li>nagy \u00e9rz\u00e9kenys\u00e9g\u0171 \u00e1raml\u00e1si citom\u00e9ter a fluoreszcens vezikul\u00e1k detekt\u00e1l\u00e1s\u00e1ra (Apogee A60MicroPlus, Apogee)<\/li>\n<li>konfok\u00e1lis mikroszk\u00f3p (Leica) az extracellul\u00e1ris vezikula termel\u00e9s \u00e9s sejtek \u00e1ltali felv\u00e9tel vizsg\u00e1lat\u00e1ra.<\/li>\n<\/ul>\n<p>A felsorolt m\u0171szerpark minden egyes tagja az \u00fczembe helyez\u00e9st k\u00f6vet\u0151en l\u00e9nyeg\u00e9ben teljes kihaszn\u00e1lts\u00e1ggal folyamatosan m\u0171k\u00f6dik. A fenti k\u00e9sz\u00fcl\u00e9kek egym\u00e1st kieg\u00e9sz\u00edt\u0151 funkci\u00f3kat l\u00e1tnak el, \u00e9s l\u00e9nyeg\u00e9ben minden extracellul\u00e1ris vezikula projekt sor\u00e1n elengedhetetlen a haszn\u00e1latuk.<\/p>\n<p>A VEKOP-2.3.3-15-2016-00016 p\u00e1ly\u00e1zati t\u00e1mogat\u00e1ssal beszerzett infrastrukt\u00far\u00e1val a projekt lez\u00e1rta ut\u00e1n:<\/p>\n<p>1. Sikeres kutat\u00e1si projektek megval\u00f3s\u00edt\u00e1sa<\/p>\n<p>2. Az extracellul\u00e1ris vezikula izol\u00e1l\u00e1sra \u00e9s m\u00e9r\u00e9sre\/jellemz\u00e9sre vonatkoz\u00f3 hazai ig\u00e9nyek szolg\u00e1ltat\u00e1sk\u00e9nt val\u00f3 kiel\u00e9g\u00edt\u00e9se<\/p>\n<p>3. Referencia laborat\u00f3riumk\u00e9nt val\u00f3 m\u0171k\u00f6d\u00e9s hazai \u00e9s k\u00fclf\u00f6ldi extracellul\u00e1ris vezikul\u00e1kat m\u00e9r\u0151 laborat\u00f3riumok, munkacsoportok eredm\u00e9nyeinek valid\u00e1l\u00e1s\u00e1ra.<\/p>\n\n<\/div><h2 class=\"tabtitle\">Beckman Coulter \u00e1ll\u00f3 ultracentrifuga<\/h2>\n<div class=\"tabcontent\">\n\n<p><em>Genetikai, sejt \u00e9s Immunbiol\u00f3giai Int\u00e9zet NET VI. emelet 603. laborat\u00f3rium<\/em><\/p>\n<p>Az ultracentrifug\u00e1k haszn\u00e1lata elengedhetetlen az extracellul\u00e1ris vezikul\u00e1k (EV-k) izol\u00e1l\u00e1s\u00e1hoz, hiszen<\/p>\n<ul>\n<li>Az EV-k nagyon apr\u00f3 r\u00e9szecsk\u00e9k, amelyeknek a hat\u00e9kony izol\u00e1l\u00e1s\u00e1hoz rendk\u00edv\u00fcl magas fordulatsz\u00e1m \u00e9s g-er\u0151 sz\u00fcks\u00e9ges. Ezt a centrifug\u00e1l\u00e1si er\u0151t hagyom\u00e1nyos centrifug\u00e1kkal lehetetlen el\u00e9rni.<\/li>\n<li>Az ultracentrifug\u00e1l\u00e1k ugyanakkor minimaliz\u00e1lja a mechanikai stresszt, amely k\u00e1ros\u00edthatn\u00e1 az EV-ket. Ez k\u00fcl\u00f6n\u00f6sen fontos, mivel az EV-k k\u00fcl\u00f6n\u00f6sen egy\u00e9bk\u00e9nt is alacsony koncentr\u00e1ci\u00f3j\u00fa, \u00e9rz\u00e9keny lipid-feh\u00e9rje strukt\u00far\u00e1k, amelyek k\u00f6nnyen s\u00e9r\u00fclhetnek.<\/li>\n<li>Az \u00e1ltal\u00e1ban relat\u00edv alacsony koncentr\u00e1ci\u00f3k mellett, az ultracentrifug\u00e1k nagy kapacit\u00e1s\u00fa rotorjai m\u00e9gis lehet\u0151v\u00e9 teszik nagy mennyis\u00e9g\u0171 minta egyidej\u0171 feldolgoz\u00e1s\u00e1t, ami hat\u00e9konyabb\u00e1 teszi az EV-k izol\u00e1l\u00e1s\u00e1t.<\/li>\n<li>Az ultracentrifug\u00e1k prec\u00edz mennyis\u00e9gekkel, nagy er\u0151kkel \u00e9s szigor\u00fa vez\u00e9rl\u00e9ssel dolgoznak, ami biztos\u00edtja az izol\u00e1l\u00e1si folyamatok reproduk\u00e1lhat\u00f3s\u00e1g\u00e1t, ami kritikus a kutat\u00e1si \u00e9s diagnosztikai alkalmaz\u00e1sokban.\u00a0<\/li>\n<\/ul>\n<p>Az EV izol\u00e1l\u00e1s alapja egy megb\u00edzhat\u00f3 ultracentrifuga, ami biztos\u00edtja vizsg\u00e1lt az extracellul\u00e1ris vezikul\u00e1k magas tisztas\u00e1g\u00e1t \u00e9s integrit\u00e1s\u00e1t a kutat\u00e1si \u00e9s klinikai alkalmaz\u00e1sokhoz.<\/p>\n<p><a href=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Ultracentrifuga.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-615\" src=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Ultracentrifuga-1024x553.jpg\" alt=\"\" width=\"753\" height=\"407\" srcset=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Ultracentrifuga-1024x553.jpg 1024w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Ultracentrifuga-400x216.jpg 400w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Ultracentrifuga-768x415.jpg 768w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Ultracentrifuga-753x407.jpg 753w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Ultracentrifuga.jpg 1400w\" sizes=\"auto, (max-width: 753px) 100vw, 753px\" \/><\/a><\/p>\n<p>Az int\u00e9zet, a VEKOP-2.3.3-15-2016-00016 p\u00e1ly\u00e1zat seg\u00edts\u00e9g\u00e9vel, egy Beckman Coulter Optima XPN ultracentrifug\u00e1t v\u00e1s\u00e1rolt:<\/p>\n<ul>\n<li>Ez egy cs\u00facstechnol\u00f3gi\u00e1s k\u00e9sz\u00fcl\u00e9k, amelyet k\u00fcl\u00f6nf\u00e9le laborat\u00f3riumi alkalmaz\u00e1sokhoz terveztek<\/li>\n<li>Maxim\u00e1lis sebess\u00e9ge 100 000 RPM<\/li>\n<li>A hozz\u00e1 tartoz\u00f3 100, Type 100 TI, TY-25 and SW-32T rotorok k\u00fcl\u00f6nb\u00f6z\u0151 l\u00e9pt\u00e9k\u0171 izol\u00e1l\u00e1sokat tesznek lehet\u0151v\u00e9.<\/li>\n<\/ul>\n<p>Mivel az tiszta, megb\u00edzhat\u00f3 izol\u00e1l\u00e1s az EV vizsg\u00e1latok els\u0151, megker\u00fclhetetlen l\u00e9p\u00e9se, az ultracentrifuga l\u00e9nyeg\u00e9ben teljes kihaszn\u00e1lts\u00e1gon folyamatosan m\u0171k\u00f6dik, \u00e9s a legt\u00f6bb kutat\u00e1s alappill\u00e9re, ha az izol\u00e1l\u00e1si l\u00e9p\u00e9s maga kev\u00e9sb\u00e9 is l\u00e1tv\u00e1nyos.<\/p>\n<h3>Publik\u00e1ci\u00f3s lista az ultracentrifuga felhaszn\u00e1l\u00e1s\u00e1val\u00a0<\/h3>\n<ul>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16660859\" target=\"_blank\" rel=\"noopener noreferrer\">Mladenovi\u0107 D, Khamari D, Kittel \u00c1, Koort K, Buz\u00e1s EI, Zarovni N. Acidification of blood plasma facilitates the separation and analysis of extracellular vesicles. J Thromb Haemost. 2023 Apr;21(4):1032\u201342.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16660864\" target=\"_blank\" rel=\"noopener noreferrer\">N\u00e9meth K, Kazsoki A, Visnovitz T, Pinke B, M\u00e9sz\u00e1ros L, Buz\u00e1s EI, et al. Nanofiber formation as a promising technology for preservation and easy storage of extracellular vesicles. Sci Rep. 2022 Dec 20;12(1):22012.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/13376958\" target=\"_blank\" rel=\"noopener noreferrer\">Hegyesi H, Pallinger \u00c9, Mecsei S, Horny\u00e1k B, Kov\u00e1csh\u00e1zi C, Brenner GB, et al. Circulating cardiomyocyte-derived extracellular vesicles reflect cardiac injury during systemic inflammatory response syndrome in mice. Cell Mol Life Sci. 2022 Jan 20;79(2):84.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16225217\" target=\"_blank\" rel=\"noopener noreferrer\">Kelemen A, Carmi I, Oszvald \u00c1, L\u0151rincz P, Pet\u0151v\u00e1ri G, T\u00f6lgyes T, et al. IFITM1 expression determines extracellular vesicle uptake in colorectal cancer. Cell Mol Life Sci. 2021 Nov;78(21\u201322):7009\u201324.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16660884\" target=\"_blank\" rel=\"noopener noreferrer\">Rzepiel A, Horv\u00e1th A, Kutszegi N, G\u00e9zsi A, S\u00e1gi JC, Alm\u00e1si L, et al. MiR-128-3p as blood based liquid biopsy biomarker in childhood acute lymphoblastic leukemia. Mol Cell Probes. 2023 Feb;67:101893.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16660875\" target=\"_blank\" rel=\"noopener noreferrer\">Koncz A, Turi\u00e1k L, N\u00e9meth K, Lenzinger D, B\u00e1rkai T, L\u0151rincz P, et al. Endoplasmin Is a Hypoxia-Inducible Endoplasmic Reticulum-Derived Cargo of Extracellular Vesicles Released by Cardiac Cell Lines. Membranes (Basel). 2023 Apr 13;13(4).<\/a><\/li>\n<\/ul>\n\n<\/div><h2 class=\"tabtitle\">Nanor\u00e9szecske sz\u00e1ml\u00e1l\u00f3 (ZetaView, ParticleMetrix)<\/h2>\n<div class=\"tabcontent\">\n\n<p><em>Genetikai, Sejt \u00e9s Immunbiol\u00f3giai Int\u00e9zet NET VI. emel et 609. laborat\u00f3rium<\/em><\/p>\n<p>A vezikulakutat\u00e1s a kih\u00edv\u00e1sa a apr\u00f3 m\u00e9ret\u0171 vezikul\u00e1k izol\u00e1l\u00e1sa mellett ezeknek a kis sejtalkot\u00f3knak az azonos\u00edt\u00e1sa. A m\u00e9ret\u00fck miatt a pontos azonos\u00edt\u00e1suk is kih\u00edv\u00e1sokkal teli feladat, ami megb\u00edzhat\u00f3an csak k\u00fcl\u00f6nb\u00f6z\u0151 egym\u00e1sra \u00e9p\u00fcl\u0151 \u00e9s egym\u00e1st kieg\u00e9sz\u00edt\u0151 technik\u00e1kkal lehets\u00e9ges. Ezekkel a technik\u00e1kkal vizsg\u00e1ljuk t\u00f6bbek k\u00f6z\u00f6tt<\/p>\n<ul>\n<li>a r\u00e9szecsk\u00e9k sz\u00e1m\u00e1t \u00e9s m\u00e9reteloszl\u00e1s\u00e1t<\/li>\n<li>felsz\u00edni markereit<\/li>\n<\/ul>\n<p>mikroszk\u00f3pos k\u00e9p\u00e9t.<\/p>\n<p><a href=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Zetaview_GSI.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-624\" src=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Zetaview_GSI-1024x364.jpg\" alt=\"\" width=\"753\" height=\"268\" srcset=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Zetaview_GSI-1024x364.jpg 1024w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Zetaview_GSI-400x142.jpg 400w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Zetaview_GSI-768x273.jpg 768w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Zetaview_GSI-753x268.jpg 753w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Zetaview_GSI.jpg 1400w\" sizes=\"auto, (max-width: 753px) 100vw, 753px\" \/><\/a><\/p>\n<p>Ezek k\u00f6z\u00fcl a r\u00e9szecsk\u00e9k sz\u00e1m\u00e1nak \u00e9s m\u00e9reteloszl\u00e1s\u00e1nak vizsg\u00e1lat\u00e1hoz az int\u00e9zet a <strong>VEKOP-2.3.3-15-2016-00016 p\u00e1ly\u00e1zat<\/strong> seg\u00edts\u00e9g\u00e9vel, ZetaView k\u00e9sz\u00fcl\u00e9ket v\u00e1s\u00e1rolt.<\/p>\n<p>A <strong>ZetaView<\/strong> egy fejlett Nanoparticle Tracking Analysis (NTA) eszk\u00f6z, amelyet a r\u00e9szecsk\u00e9k hidrodinamikai m\u00e9ret\u00e9nek, z\u00e9ta potenci\u00e1lj\u00e1nak \u00e9s koncentr\u00e1ci\u00f3j\u00e1nak m\u00e9r\u00e9s\u00e9re terveztek. K\u00e9pes t\u00f6bbek k\u00f6z\u00f6tt<\/p>\n<ul>\n<li>A R\u00e9szecsk\u00e9k egyedi k\u00f6vet\u00e9s\u00e9re klasszikus mikro-elektrofor\u00e9zis \u00e9s Brown-mozg\u00e1s alkalmaz\u00e1s\u00e1val.<\/li>\n<li>Az EV-k m\u00e9ret\u00e9t \u00e9s koncentr\u00e1ci\u00f3j\u00e1t pontos meghat\u00e1roz\u00e1s\u00e1ra, ami alapvet\u0151 fontoss\u00e1g\u00fa a kvantitat\u00edv elemz\u00e9sekhez.<\/li>\n<li>Z\u00e9ta potenci\u00e1l m\u00e9r\u00e9s\u00e9re. A z\u00e9ta potenci\u00e1l a r\u00e9szecsk\u00e9k fel\u00fcleti t\u00f6lt\u00e9s\u00e9r\u0151l \u00e9s stabilit\u00e1s\u00e1r\u00f3l ad inform\u00e1ci\u00f3t. Ez k\u00fcl\u00f6n\u00f6sen fontos a vezikul\u00e1k biol\u00f3giai funkci\u00f3inak \u00e9s interakci\u00f3inak meg\u00e9rt\u00e9s\u00e9hez.<\/li>\n<li>Fluoreszcens r\u00e9szecsk\u00e9k k\u00f6vet\u00e9s\u00e9re \u00e9s elemz\u00e9s\u00e9re k\u00fcl\u00f6nb\u00f6z\u0151 hull\u00e1mhosszokon.<\/li>\n<li>Automatikus igaz\u00edt\u00e1sra \u00e9s f\u00f3kusz\u00e1l\u00e1sra, ami biztos\u00edtja a k\u00f6nny\u0171 kezelhet\u0151s\u00e9get \u00e9s a megb\u00edzhat\u00f3 eredm\u00e9nyeket.<\/li>\n<\/ul>\n<p>A k\u00e9sz\u00fcl\u00e9k k\u00e9pes z\u00e9ta potenci\u00e1l m\u00e9r\u00e9s\u00e9re \u00e9s az al\u00e1bbi l\u00e9zert tartalmazza: 520 nm 35mW<\/p>\n<p><strong>Publik\u00e1ci\u00f3s lista<\/strong> a Zetaview felhaszn\u00e1l\u00e1s\u00e1val (b\u0151v\u00fcl\u0151 lista):<\/p>\n<ol>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16225217\" target=\"_blank\" rel=\"noopener noreferrer\">Kelemen A, Carmi I, Oszvald \u00c1, L\u0151rincz P, Pet\u0151v\u00e1ri G, T\u00f6lgyes T, et al. IFITM1 expression determines extracellular vesicle uptake in colorectal cancer. Cell Mol Life Sci. 2021 Nov;78(21\u201322):7009\u201324.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16661334\" target=\"_blank\" rel=\"noopener noreferrer\">N\u00e9meth K, Varga Z, Lenzinger D, Visnovitz T, Koncz A, Heged\u0171s N, et al. Extracellular vesicle release and uptake by the liver under normo- and hyperlipidemia. Cell Mol Life Sci. 2021 Dec;78(23):7589\u2013604.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/13376958\" target=\"_blank\" rel=\"noopener noreferrer\">Hegyesi H, Pallinger \u00c9, Mecsei S, Horny\u00e1k B, Kov\u00e1csh\u00e1zi C, Brenner GB, et al. Circulating cardiomyocyte-derived extracellular vesicles reflect cardiac injury during systemic inflammatory response syndrome in mice. Cell Mol Life Sci. 2022 Jan 20;79(2):84.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16660864\" target=\"_blank\" rel=\"noopener noreferrer\">N\u00e9meth K, Kazsoki A, Visnovitz T, Pinke B, M\u00e9sz\u00e1ros L, Buz\u00e1s EI, et al. Nanofiber formation as a promising technology for preservation and easy storage of extracellular vesicles. Sci Rep. 2022 Dec 20;12(1):22012.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16660859\" target=\"_blank\" rel=\"noopener noreferrer\">Mladenovi\u0107 D, Khamari D, Kittel \u00c1, Koort K, Buz\u00e1s EI, Zarovni N. Acidification of blood plasma facilitates the separation and analysis of extracellular vesicles. J Thromb Haemost. 2023 Apr;21(4):1032\u201342.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16660875\" target=\"_blank\" rel=\"noopener noreferrer\">Koncz A, Turi\u00e1k L, N\u00e9meth K, Lenzinger D, B\u00e1rkai T, L\u0151rincz P, et al. Endoplasmin Is a Hypoxia-Inducible Endoplasmic Reticulum-Derived Cargo of Extracellular Vesicles Released by Cardiac Cell Lines. Membranes (Basel). 2023 Apr 13;13(4).<\/a><\/li>\n<\/ol>\n\n<\/div><h2 class=\"tabtitle\">Nagy \u00e9rz\u00e9kenys\u00e9g\u0171 \u00e1raml\u00e1si citom\u00e9ter (ApogeeA60 MicroPlus)<\/h2>\n<div class=\"tabcontent\">\n\n<p>Genetikai, Sejt \u00e9s Immunbiol\u00f3giai Int\u00e9zet NET VI. emel et 609. laborat\u00f3rium<\/p>\n<p>A vezikul\u00e1k apr\u00f3 m\u00e9ret\u00fckb\u0151l ad\u00f3d\u00f3an, hagyom\u00e1nyos m\u00f3dszerekkel sokszor nehezen elemezhet\u0151ek, ez\u00e9rt az igaz\u00e1n hat\u00e9kony, meggy\u0151z\u0151 vizsg\u00e1latuk k\u00fcl\u00f6nb\u00f6z\u0151 \u00e1raml\u00e1si citom\u00e9terekkel t\u00f6rt\u00e9nik. Az EV-k vizsg\u00e1lat\u00e1ra haszn\u00e1lhat\u00f3 citom\u00e9ternek k\u00e9pesnek kell lennie<\/p>\n<ul>\n<li>Kiv\u00e9teles \u00e9rz\u00e9kenys\u00e9gre: az EV-ka apr\u00f3k, kev\u00e9s f\u00e9nyt sz\u00f3rnak, a sejtt\u00f6rmel\u00e9kt\u0151l \u00e9s feh\u00e9rje aggreg\u00e1tumokt\u00f3l sokszor nehezen elk\u00fcl\u00f6n\u00edthet\u0151k<\/li>\n<li>nagy sebess\u00e9gr\u0171 esem\u00e9nyr\u00f6gz\u00edt\u00e9sre: m\u00e1sodpercenk\u00e9nt t\u00f6bb t\u00edz, vagy ak\u00e1r sz\u00e1zezer r\u00e9szecske is elhaladhat az \u00e9rz\u00e9kel\u0151k el\u0151tt, ezeket nagy sz\u00e1mban k\u00e9pesnek kell lennie az eszk\u00f6znek detekt\u00e1lni<\/li>\n<li>automatiz\u00e1lhat\u00f3s\u00e1g: mind a m\u00e9r\u00e9si, mind a tiszt\u00edt\u00e1si l\u00e9p\u00e9sekben, ami cs\u00f6kkenti az emberi beavatkoz\u00e1s m\u00e9rt\u00e9k\u00e9t, \u00e9s n\u00f6veli a m\u00e9r\u00e9sek reproduk\u00e1lhat\u00f3s\u00e1g\u00e1t.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Apogee_GSI.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-625\" src=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Apogee_GSI-1024x314.jpg\" alt=\"\" width=\"753\" height=\"231\" srcset=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Apogee_GSI-1024x314.jpg 1024w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Apogee_GSI-400x123.jpg 400w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Apogee_GSI-768x235.jpg 768w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Apogee_GSI-753x231.jpg 753w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/Apogee_GSI.jpg 1400w\" sizes=\"auto, (max-width: 753px) 100vw, 753px\" \/><\/a><\/p>\n<p>Az int\u00e9zet EV-k ilyenforma vizsg\u00e1lat\u00e1ra, a <strong>VEKOP-2.3.3-15-2016-00016 p\u00e1ly\u00e1zat<\/strong> seg\u00edts\u00e9g\u00e9vel, Apogee A60-Micro-PLUS cs\u00facstechnol\u00f3gi\u00e1s \u00e1raml\u00e1si citom\u00e9ter v\u00e1s\u00e1rolt, amelyet kifejezetten kis r\u00e9szecsk\u00e9k, t\u00f6bbek k\u00f6z\u00f6tt extracellul\u00e1ris vezikul\u00e1k m\u00e9r\u00e9s\u00e9re fejlesztettek.<\/p>\n<p>Az eszk\u00f6z:<\/p>\n<ul>\n<li><strong>Kiv\u00e9teles \u00e9rz\u00e9kenys\u00e9ggel rendelkezik<\/strong>: ak\u00e1r 110 nm \u00e1tm\u00e9r\u0151j\u0171 r\u00e9szecsk\u00e9k m\u00e9r\u00e9s\u00e9re is alkalmas, ami k\u00fcl\u00f6n\u00f6sen kiemeli a hasonl\u00f3 m\u0171szerek k\u00f6z\u00fcl, \u00e9s a legkritikusabb tartom\u00e1nyban pontosabb m\u00e9r\u00e9seket tesz lehet\u0151v\u00e9.<\/li>\n<li><strong>Nagy sebess\u00e9g<\/strong>: Ak\u00e1r 100 000 esem\u00e9nyt is k\u00e9pes r\u00f6gz\u00edteni m\u00e1sodpercenk\u00e9nt<\/li>\n<li><strong>Automatiz\u00e1lhat\u00f3 a m\u00e9r\u00e9s \u00e9s a tiszt\u00edt\u00e1s<\/strong><\/li>\n<li><strong>Magas \u00e1tereszt\u0151k\u00e9pess\u00e9g\u0171 \u00e9s reproduk\u00e1lhat\u00f3 m\u00e9r\u00e9seket tesz lehet\u0151v\u00e9<\/strong><\/li>\n<li><strong>Felhaszn\u00e1l\u00f3bar\u00e1t szoftverrel \u00e9rkezik,<\/strong> ami megk\u00f6nny\u00edti a eredm\u00e9nyek \u00e9rtelmez\u00e9s\u00e9t \u00e9s ki\u00e9rt\u00e9kel\u00e9s\u00e9t.<\/li>\n<\/ul>\n<p>A k\u00e9sz\u00fcl\u00e9k jelenleg az al\u00e1bbi l\u00e9zereket tartalmazza: 405 nm, 488 nm \u00e9s 638 nm<\/p>\n<p><strong>Publik\u00e1ci\u00f3s lista<\/strong> az Apogee felhaszn\u00e1l\u00e1s\u00e1val (b\u0151v\u00fcl\u0151 lista):<\/p>\n<ol>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16660875\" target=\"_blank\" rel=\"noopener noreferrer\">Koncz A, Turi\u00e1k L, N\u00e9meth K, Lenzinger D, B\u00e1rkai T, L\u0151rincz P, et al. Endoplasmin Is a Hypoxia-Inducible Endoplasmic Reticulum-Derived Cargo of Extracellular Vesicles Released by Cardiac Cell Lines. Membranes (Basel). 2023 Apr 13;13(4).<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/14926663\" target=\"_blank\" rel=\"noopener noreferrer\">Bognar Z, Csabai TJ, Pallinger E, Balassa T, Farkas N, Schmidt J, et al. The effect of light exposure on the cleavage rate and implantation capacity of preimplantation murine embryos. J Reprod Immunol. 2019 Apr;132:21\u20138.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/11419340\" target=\"_blank\" rel=\"noopener noreferrer\">Kov\u00e1cs \u00c1F, L\u00e1ng O, Turi\u00e1k L, \u00c1cs A, K\u0151hidai L, Fekete N, et al. The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells. Sci Rep. 2018 Apr 3;8(1):5426.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/4999838\" target=\"_blank\" rel=\"noopener noreferrer\">Pallinger E, Bognar Z, Bogdan A, Csabai T, Abraham H, Szekeres-Bartho J. PIBF+ extracellular vesicles from mouse embryos affect IL-10 production by CD8+ cells. Sci Rep. 2018 Mar 16;8(1):4662.<\/a><\/li>\n<\/ol>\n\n<\/div><h2 class=\"tabtitle\">L\u00e9zer konfok\u00e1lis mikroszk\u00f3p (Leica)<\/h2>\n<div class=\"tabcontent\">\n\n<p>Farmakol\u00f3giai \u00e9s Farmakoter\u00e1pi\u00e1s Int\u00e9zet IV. emelet 403. laborat\u00f3rium<\/p>\n<p>A vezikula kutat\u00e1sban a r\u00e9szecsk\u00e9k vizualiz\u00e1ci\u00f3ja a valid\u00e1ci\u00f3 egyik m\u00f3dja, de mag\u00e1b\u00f3l a vizualiz\u00e1ci\u00f3b\u00f3l, lokaliz\u00e1ci\u00f3b\u00f3l, fluoreszcens jel\u00f6l\u00e9sb\u0151l olyan biol\u00f3giai inform\u00e1ci\u00f3k is nyerhet\u0151k, amik az izol\u00e1lt vezikul\u00e1k vizsg\u00e1lat\u00e1val nem megszerezhet\u0151k.<\/p>\n<p>A konfok\u00e1lis mikroszk\u00f3pok l\u00e9zerf\u00e9ny seg\u00edts\u00e9g\u00e9vel pontr\u00f3l pontra p\u00e1szt\u00e1zz\u00e1k a mint\u00e1t, \u00edgy nagy felbont\u00e1s\u00fa (ak\u00e1r 0,1 mikrom\u00e9teres), h\u00e1romdimenzi\u00f3s k\u00e9peket k\u00e9sz\u00edt relat\u00edv alacsony h\u00e1tt\u00e9rzajjal \u00e9s magas kontraszttal. Fluoreszcencia alap\u00fa k\u00e9palkot\u00e1ssal kieg\u00e9sz\u00edtve pedig lehet\u0151s\u00e9g ny\u00edlik specifikus molekul\u00e1k \u00e9s strukt\u00far\u00e1k megjel\u00f6l\u00e9s\u00e9t \u00e9s vizsg\u00e1lat\u00e1t a mint\u00e1ban.<\/p>\n<p><a href=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/konfokalis_leica_mikroszkop_GSI.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-626\" src=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/konfokalis_leica_mikroszkop_GSI-1024x500.jpg\" alt=\"\" width=\"753\" height=\"368\" srcset=\"https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/konfokalis_leica_mikroszkop_GSI-1024x500.jpg 1024w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/konfokalis_leica_mikroszkop_GSI-400x195.jpg 400w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/konfokalis_leica_mikroszkop_GSI-768x375.jpg 768w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/konfokalis_leica_mikroszkop_GSI-753x367.jpg 753w, https:\/\/semmelweis.hu\/gsi\/files\/2026\/08\/konfokalis_leica_mikroszkop_GSI.jpg 1152w\" sizes=\"auto, (max-width: 753px) 100vw, 753px\" \/><\/a><\/p>\n<p>EV-k vizualiz\u00e1ci\u00f3j\u00e1hoz az int\u00e9zet a <strong>VEKOP-2.3.3-15-2016-00016 p\u00e1ly\u00e1zat<\/strong> seg\u00edts\u00e9g\u00e9vel, <strong>Leica l\u00e9zer konfok\u00e1lis mikroszk\u00f3pot v\u00e1s\u00e1rolt. A mikroszk\u00f3p k\u00e9pes<\/strong><\/p>\n<ul>\n<li><strong>Nagy felbont\u00e1s\u00fa k\u00e9palkot\u00e1sra<\/strong>, ami lehet\u0151v\u00e9 teszi a vezikul\u00e1k morfol\u00f3gi\u00e1j\u00e1nak \u00e9s m\u00e9ret\u00e9nek pontos meghat\u00e1roz\u00e1s\u00e1hoz.<\/li>\n<li><strong>Fluoreszcencia k\u00e9palkot\u00e1sra<\/strong>, ami lehet\u0151v\u00e9 teszi az EV-k specifikus jel\u00f6l\u00e9s\u00e9t \u00e9s vizsg\u00e1lat\u00e1t, ezzel pedig az EV-k k\u00fcl\u00f6nb\u00f6z\u0151 popul\u00e1ci\u00f3inak azonos\u00edt\u00e1s\u00e1t \u00e9s jellemz\u00e9s\u00e9t.<\/li>\n<li><strong>H\u00e1romdimenzi\u00f3s rekonstrukci\u00f3ra<\/strong>, ahol a mikroszk\u00f3p h\u00e1romdimenzi\u00f3s k\u00e9peket k\u00e9sz\u00edt, az EV-k t\u00e9rbeli eloszl\u00e1s\u00e1nak \u00e9s interakci\u00f3inak vizsg\u00e1lat\u00e1hoz.<\/li>\n<li><strong>Automatiz\u00e1ci\u00f3ra<\/strong>, ahol a p\u00e1szt\u00e1z\u00e1si \u00e9s f\u00f3kusz\u00e1l\u00e1si funkci\u00f3k n\u00f6velik a m\u00e9r\u00e9si pontoss\u00e1got \u00e9s cs\u00f6kkentik a felhaszn\u00e1l\u00f3i hib\u00e1kat.<\/li>\n<\/ul>\n<p>A k\u00e9sz\u00fcl\u00e9k k\u00e9pes z\u00e9ta potenci\u00e1l m\u00e9r\u00e9s\u00e9re \u00e9s az al\u00e1bbi l\u00e9zereket tartalmazza: Laser 405 nm, Laser Blue 488 nm, Laser Green 552 nm, Laser Red 638 nm<\/p>\n<p><strong>Publik\u00e1ci\u00f3s lista<\/strong> a konfok\u00e1lis mikroszk\u00f3p felhaszn\u00e1l\u00e1s\u00e1val (b\u0151v\u00fcl\u0151 lista):<\/p>\n<ol>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16661334\" target=\"_blank\" rel=\"noopener noreferrer\">N\u00e9meth K, Varga Z, Lenzinger D, Visnovitz T, Koncz A, Heged\u0171s N, et al. Extracellular vesicle release and uptake by the liver under normo- and hyperlipidemia. Cell Mol Life Sci. 2021 Dec;78(23):7589\u2013604.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/16225217\" target=\"_blank\" rel=\"noopener noreferrer\">Kelemen A, Carmi I, Oszvald \u00c1, L\u0151rincz P, Pet\u0151v\u00e1ri G, T\u00f6lgyes T, et al. IFITM1 expression determines extracellular vesicle uptake in colorectal cancer. Cell Mol Life Sci. 2021 Nov;78(21\u201322):7009\u201324.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/10157550\" target=\"_blank\" rel=\"noopener noreferrer\">G\u00e1l Z, G\u00e9zsi A, P\u00e1llinger \u00c9, Visnovitz T, Nagy A, Kiss A, et al. Plasma neutrophil extracellular trap level is modified by disease severity and inhaled corticosteroids in chronic inflammatory lung diseases. Sci Rep. 2020 Mar 9;10(1):4320.<\/a><\/li>\n<li><a href=\"https:\/\/sciwheel.com\/work\/bibliography\/12900292\" target=\"_blank\" rel=\"noopener noreferrer\">On\u00f3di Z, Visnovitz T, Kiss B, Hambalk\u00f3 S, Koncz A, \u00c1gg B, et al. Systematic transcriptomic and phenotypic characterization of human and murine cardiac myocyte cell lines and primary cardiomyocytes reveals serious limitations and low resemblances to adult cardiac phenotype. J Mol Cell Cardiol. 2022 Apr;165:19\u201330.<\/a><\/li>\n<\/ol>\n<\/div><\/div>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Magyar K\u00f6zponti Extracellul\u00e1ris Vezikula Kutat\u00f3, Szolg\u00e1ltat\u00f3 \u00e9s Referencia Laborat\u00f3rium l\u00e9trehoz\u00e1sa a Semmelweis Egyetemen<\/p>\n","protected":false},"author":102560,"featured_media":0,"parent":149,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[],"class_list":["post-588","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/gsi\/wp-json\/wp\/v2\/pages\/588","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/gsi\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/semmelweis.hu\/gsi\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/gsi\/wp-json\/wp\/v2\/users\/102560"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/gsi\/wp-json\/wp\/v2\/comments?post=588"}],"version-history":[{"count":10,"href":"https:\/\/semmelweis.hu\/gsi\/wp-json\/wp\/v2\/pages\/588\/revisions"}],"predecessor-version":[{"id":686,"href":"https:\/\/semmelweis.hu\/gsi\/wp-json\/wp\/v2\/pages\/588\/revisions\/686"}],"up":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/gsi\/wp-json\/wp\/v2\/pages\/149"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/gsi\/wp-json\/wp\/v2\/media?parent=588"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/gsi\/wp-json\/wp\/v2\/categories?post=588"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/gsi\/wp-json\/wp\/v2\/tags?post=588"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}