{"id":16418,"date":"2024-03-26T15:32:34","date_gmt":"2024-03-26T14:32:34","guid":{"rendered":"https:\/\/semmelweis.hu\/corefacility\/?page_id=16418"},"modified":"2026-01-15T14:30:21","modified_gmt":"2026-01-15T13:30:21","slug":"proteomics-sample-preparation-for-mass-spectrometry-analysis","status":"publish","type":"page","link":"https:\/\/semmelweis.hu\/corefacility\/home\/proteomics-sample-preparation-for-mass-spectrometry-analysis\/","title":{"rendered":"Proteomics sample preparation for mass spectrometry analysis"},"content":{"rendered":"<div class=\"keretes\" style=\"width: 40% !important;border-left: 6px solid #4d9de0 !important\">\n<p><strong>Head of Core Facility<br \/>\n<\/strong>Krisztina, Lud\u00e1nyi Dr.<\/p>\n<p><strong>Core Facility Address<br \/>\n<\/strong>Department of Pharmaceutics<br \/>\nH\u0151gyes E. u. 7.<br \/>\nBudapest, Hungary<br \/>\nH-1092<\/p>\n<p><strong>Contact E-mail<br \/>\n<\/strong>M\u00e1rton Kir\u00e1ly Dr.<br \/>\n<a href=\"mailto:kiraly.marton@semmelweis.hu\" target=\"_blank\" rel=\"noopener\">kiraly.marton@semmelweis.hu<\/a><\/p>\n<p><strong>Staff<br \/>\n<\/strong>4<\/p>\n<p><strong>Quality Control<br \/>\n<\/strong>ISO90001<\/p>\n<\/div>\n<h4><strong>What we offer<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>Protein extraction from biological samples<\/li>\n<li>Protein purification<\/li>\n<li>Sample preparation for mass spectrometry-based proteomic\/glycoproteomic analysis<\/li>\n<li>Estimation of protein purity and protein quantity in samples<\/li>\n<li>Release and derivatization of N-oligosaccharides for glycomic analysis<\/li>\n<\/ul>\n<h4><strong>Why work with us<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>Our experienced team has a strong backround in proteomics and several key equipment to prepare samples for the mass spectrometry-based qualitative and quantitative analysis of protein\/glycoprotein\/glycan samples.<\/li>\n<\/ul>\n<h4><strong>Our equipment<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>Pharmacia FPLC system equipped with UV detector<\/li>\n<li>Agilent 1100 HPLC system with DAD and fluorescent detector<\/li>\n<li>Uvitec Cambridge Gel electrophoresis and documentation system<\/li>\n<li>Labogene Vacuum ceoncentrator and liophylizator<\/li>\n<li>Grant-bio PCH-3 Block thermostat<\/li>\n<li>Centrifuges<\/li>\n<li>Semi-preparative chromatography columns (size exclusion, ion-exchange, affinity)<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/semmelweis.hu\/corefacility\/files\/2024\/04\/Proteomics-core-facility-abra.jpg\" alt=\"\" width=\"800\" height=\"1400\" \/><\/p>\n<h4><strong>References<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>DOI: 10.1007\/s10337-019-03796-9<\/li>\n<li>DOI: 10.3390\/molecules26196003<\/li>\n<li>DOI: 10.1016\/j.btre.2021.e00637<\/li>\n<li>DOI: 10.3390\/ijms25094656<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Head of Core Facility Krisztina, Lud\u00e1nyi Dr. Core Facility Address Department of Pharmaceutics H\u0151gyes E. u. 7. Budapest, Hungary H-1092 Contact E-mail M\u00e1rton Kir\u00e1ly Dr. <a href=\"mailto:kiraly.marton@semmelweis.hu\" target=\"_blank\" rel=\"noopener\">kiraly.marton@semmelweis.hu<\/a> Staff 4 Quality Control ISO90001 What we offer Protein extraction from biological samples Protein purification Sample preparation for mass spectrometry-based proteomic\/glycoproteomic analysis Estimation of protein purity and protein quantity in &hellip;<\/p>\n","protected":false},"author":102026,"featured_media":16459,"parent":14502,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-fullwidth.php","meta":{"_acf_changed":false,"footnotes":""},"categories":[15,37],"tags":[38,39,41,40],"class_list":["post-16418","page","type-page","status-publish","has-post-thumbnail","hentry","category-core-facility","category-medicinal-chemistry","tag-glycoproteomics","tag-mass-spectrometry","tag-proteomics","tag-sample-preparation"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/16418","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/users\/102026"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/comments?post=16418"}],"version-history":[{"count":9,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/16418\/revisions"}],"predecessor-version":[{"id":16565,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/16418\/revisions\/16565"}],"up":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/14502"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media\/16459"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media?parent=16418"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/categories?post=16418"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/tags?post=16418"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}