{"id":15810,"date":"2023-05-08T16:00:48","date_gmt":"2023-05-08T14:00:48","guid":{"rendered":"https:\/\/semmelweis.hu\/corefacility\/?page_id=15810"},"modified":"2024-11-07T16:03:25","modified_gmt":"2024-11-07T15:03:25","slug":"viral-vector-core","status":"publish","type":"page","link":"https:\/\/semmelweis.hu\/corefacility\/home\/viral-vector-core\/","title":{"rendered":"Viral Vector Core"},"content":{"rendered":"<div class=\"keretes\" style=\"border-left: 6px solid #4d9de0 !important\">\n<p><strong>Head of Core Facility<br \/>\n<\/strong>Karolina Pircs, PhD<\/p>\n<p><strong>Core Facility Address<br \/>\n<\/strong>Basic Medical Science Center<br \/>\nInstitute of Translational Medicine<br \/>\nSemmelweis University<br \/>\n0.112 laboratory room<br \/>\nD part, ground floor<br \/>\nT\u0171zolt\u00f3 street. 37\u201347.<br \/>\n1094 Budapest<\/p>\n<p><strong>Contact E-mail<br \/>\n<\/strong>to: \u00c1gnes Varga, PhD<br \/>\n<a href=\"mailto:varga.agnes1@semmelweis.hu\">varga.agnes1@semmelweis.hu<\/a><br \/>\ncc: Karolina Pircs, PhD<br \/>\n<a href=\"mailto:pircs.karolina@semmelweis.hu\">pircs.karolina@semmelweis.hu<\/a><\/p>\n<p><strong>Number of staff<br \/>\n<\/strong>3<\/p>\n<p><strong>Quality Control<br \/>\n<\/strong>ISO 9001<\/p>\n<\/div>\n<h4><strong>What we offer<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>Generation of lentiviral vectors (overexpression of cDNA, shRNA, miRNA, etc.)\n<ul style=\"list-style-type: circle\">\n<li>Design and cloning<\/li>\n<li>Production, titration and quality control (QC) of viral vectors<\/li>\n<\/ul>\n<\/li>\n<li>Generation of CRISPR\/CAS9 lentiviral vectors for gene modification, gene therapy\u00a0CRISPR KO, CRISPRi (CRISPR interference), CRISPRa (CRISPR activation)\n<ul style=\"list-style-type: circle\">\n<li>Design and cloning<\/li>\n<li>Production, titration and quality control (QC) of viral vectors<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h4><strong>Why work with us<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>Unique service for academic researchers in the country <br \/>\n(only available at Our University)<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h4><strong>equipmentS<\/strong><\/h4>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignright\" src=\"https:\/\/semmelweis.hu\/corefacility\/files\/2021\/12\/Karolina_Pircs_Lentivirus_Production.png\" alt=\"\" width=\"454\" height=\"642\" \/><\/p>\n<ul style=\"list-style-type: square\">\n<li>BSL-2 cell culturing laboratory with Biobase Class II A2 Biological Safety Cabinet\n<\/li>\n<\/ul>\n<table width=\"447\">\n<tbody>\n<tr>\n<td width=\"447\">4 pcs. CCL-170B-8 CelCulture Incubator 170L IR Sensor CO2 Control ULPA, Moist Heat Decon 230VAC 50\/60HZ incubators (with GASGUARD 3250)<\/td>\n<\/tr>\n<tr>\n<td width=\"447\">SW-32TI \u00a0Beckman&amp;Coulter Rotor for Ultracentrifugation<\/td>\n<\/tr>\n<tr>\n<td width=\"447\">734-3333 EVE\u2122 Plus automata cell counter<\/td>\n<\/tr>\n<tr>\n<td width=\"447\">Bio-Rad CFX Opus 384 Real-Time PCR System<\/td>\n<\/tr>\n<tr>\n<td width=\"447\">VWR Chemi Premium gel-documentation system<\/td>\n<\/tr>\n<tr>\n<td width=\"447\">Nuer -86C\u00a0Ultralow Freezer wit HC<\/td>\n<\/tr>\n<tr>\n<td width=\"447\">Pipettig robot: 96 Channel Disposable Tip Head; ST Liquid handling pipetting head for use with Agilent Liquid Handling Station<\/td>\n<\/tr>\n<tr>\n<td width=\"447\">NanoDrop OneC Spectrophotometer<\/td>\n<\/tr>\n<tr>\n<td width=\"447\">Optima xpn-80 ultracentrifuge<\/td>\n<\/tr>\n<tr>\n<td width=\"447\">Zeiss Axiovert 40 CFL\u00a0Inverted Fluorescence Phase Contrast Microscope Pred Axio Vert.A1<\/td>\n<\/tr>\n<tr>\n<td width=\"447\">VWR Mega Star 600R refrigerated centrifuge<\/td>\n<\/tr>\n<tr>\n<td width=\"447\">VWR Micro Star 30R refrigerated microcentrifuge<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h4><strong>References <\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>G\u00e1bor Juh\u00e1sz (Momentum-BRC)<\/li>\n<li>Lajos Kem\u00e9ny (HCEMM-SU)<\/li>\n<li>\u00c1kos Zsembery (SU)<\/li>\n<li>Zolt\u00e1n Beny\u00f3 (SU)<\/li>\n<li>Zolt\u00e1n Giricz (SU)<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Head of Core Facility Karolina Pircs, PhD Core Facility Address Basic Medical Science Center Institute of Translational Medicine Semmelweis University 0.112 laboratory room D part, ground floor T\u0171zolt\u00f3 street. 37\u201347. 1094 Budapest Contact E-mail to: \u00c1gnes Varga, PhD <a href=\"mailto:varga.agnes1@semmelweis.hu\">varga.agnes1@semmelweis.hu<\/a> cc: Karolina Pircs, PhD <a href=\"mailto:pircs.karolina@semmelweis.hu\">pircs.karolina@semmelweis.hu<\/a> Number of staff 3 Quality Control ISO 9001 What we offer Generation of &hellip;<\/p>\n","protected":false},"author":102026,"featured_media":15408,"parent":14502,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-fullwidth.php","meta":{"_acf_changed":false,"footnotes":""},"categories":[15,18],"tags":[],"class_list":["post-15810","page","type-page","status-publish","has-post-thumbnail","hentry","category-core-facility","category-molecular-biology"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15810","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/users\/102026"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/comments?post=15810"}],"version-history":[{"count":10,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15810\/revisions"}],"predecessor-version":[{"id":16508,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15810\/revisions\/16508"}],"up":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/14502"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media\/15408"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media?parent=15810"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/categories?post=15810"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/tags?post=15810"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}