{"id":15765,"date":"2023-05-08T14:37:33","date_gmt":"2023-05-08T12:37:33","guid":{"rendered":"https:\/\/semmelweis.hu\/corefacility\/?page_id=15765"},"modified":"2024-11-07T16:02:04","modified_gmt":"2024-11-07T15:02:04","slug":"ngs-sequencer","status":"publish","type":"page","link":"https:\/\/semmelweis.hu\/corefacility\/home\/ngs-sequencer\/","title":{"rendered":"Next Generation Sequencing"},"content":{"rendered":"<div class=\"keretes\" style=\"border-left: 6px solid #4d9de0 !important\">\n<p><strong>Head of Core Facility<br \/>\n<\/strong>Gy\u00f6ngyi Munk\u00e1csy, PhD<\/p>\n<p><strong>Core Facility Address<br \/>\n<\/strong>Department of Bioinformatics<br \/>\nT\u0171zolt\u00f3 u. 7.<br \/>\n1094 Budapest<\/p>\n<p><strong>Contact E-mail<br \/>\n<\/strong>to: Gy\u00f6ngyi Munk\u00e1csy, PhD<strong><br \/>\n<\/strong><a href=\"mailto:munkacsy.gyongyi@semmelweis.hu\" target=\"_blank\" rel=\"noopener\">munkacsy.gyongyi@semmelweis.hu<\/a><\/p>\n<p><strong>Staff<br \/>\n<\/strong>4<\/p>\n<p><strong>Quality Control<br \/>\n<\/strong>ISO 9001<\/p>\n<\/div>\n<h4><strong>What we offer<\/strong><\/h4>\n<p>The NGS Core Facility offers Next Generation Sequencing Service that combines tried-and-true instrument technologies and tunable output with sequencing capabilities. Users can run transcriptome, targeted sequencing, genotyping, and other applications all on one platform. Data analysis software is also available for successful evaluation of the results.<\/p>\n<p>&nbsp;<\/p>\n<h4><strong>Why work with us<\/strong><\/h4>\n<p>Our NGS Core Facility provides Next-Generation Sequencing service with all instruments and softwares needed for successful results from the isolation of the nucleic acids through quality and quantity controls to the final analysis of NGS data.<\/p>\n<p>&nbsp;<\/p>\n<h4><strong>equipment<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>QIACube Connect &#8211; Fully automated purification of nucleic acids and proteins<\/li>\n<li>Invitrogen Qubit 3 \u2013 Fluorometer<\/li>\n<li>Fragment Analyzer &#8211; Capillary electrophoresis<\/li>\n<li>Tapestation 4150 &#8211; Automated electrophoresis system<\/li>\n<li>Bioruptor Pico &#8211; Sonication shearing system<\/li>\n<li>Illumina NextSeq 500 &#8211; NGS sequencer<\/li>\n<li>Semmelweis GALAXY platform \u2013 software for the analysis of NGS data<\/li>\n<li>Illumina NextSeq 500 \u2013 Next Generation Sequencer<\/li>\n<\/ul>\n<h4><strong>References<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>\u00a0<\/li>\n<li>\u00a0<\/li>\n<\/ul>\n\r\n        [slb_exclude]\r\n        <div class=\"row galeria-wrapper mx-0 h-auto\">\r\n            <div class=\"col-9 pl-0 pr-2\">\r\n            <span class=\"gallery-item frame\">\r\n\t\t\t\t<div class=\"galeria-left-img\"><a href=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Biorutor_Pico.jpg\"><img src=https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Biorutor_Pico.jpg  \r\n                            alt=\"\" \r\n                            class=\"attachment-sote-gallery-big size-sote-gallery-big\" \r\n                            srcset=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Biorutor_Pico-400x300.jpg 400w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Biorutor_Pico-300x225.jpg 300w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Biorutor_Pico.jpg 567w\" ><\/a><\/div>\r\n            <\/span>\r\n\r\n            <\/div>\r\n\r\n\t\t\t<div class=\"col-3 pl-0 pr-0\">\r\n\t\t\t\t<div class=\"galeria-right-img d-flex justify-content-between flex-column h-100 w-100 px-0 mx-0 \">\r\n                <div class=\"galeria-box\">\r\n\t\t\t\t\t<h3 class=\"galeria-title\">Gallery<\/h3>\r\n\t\t\t\t\t<div class=\"galeria-subtitle\">\r\n\r\n\t\t\t\t\t\t<div class=\"galeria-page-counter\"><span>8<\/span>images<\/div>\r\n                    <\/div> <\/div>\r\n                    <span class=\"gallery-item frame d-none d-md-flex\">\r\n\t\t\t\t\t<div class=\"galeria-small-img mt-0\"><a href=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Slide_Galaxy.png\"><img src=https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Slide_Galaxy.png \r\n                                alt=\"\"  \r\n                                class=\"attachment-sote-gallery-small size-sote-gallery-small\" \r\n                                srcset=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Slide_Galaxy-400x131.png 400w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Slide_Galaxy-768x251.png 768w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Slide_Galaxy-1024x335.png 1024w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Slide_Galaxy-1536x503.png 1536w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Slide_Galaxy-2048x670.png 2048w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Slide_Galaxy-753x246.png 753w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Slide_Galaxy-300x98.png 300w\" ><\/a><\/div>\r\n                    <\/span>\r\n                    <span class=\"gallery-item frame d-none d-md-flex\">\r\n\t\t\t\t\t<div class=\"galeria-small-img mt-0\"><a href=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Illumina-NextSeq-500.png\"><img src=https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Illumina-NextSeq-500.png \r\n                                alt=\"\" \r\n                                class=\"attachment-sote-gallery-small size-sote-gallery-small\" \r\n                                srcset=\"\" ><\/a><\/div>\r\n                    <\/span>\r\n\r\n                <\/div>\r\n\r\n            <\/div>\r\n\r\n            <span class=\"gallery-item\"><div class=\"hidden\"><a href=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Bioruptor-Pico-e1676899111358.jpg\">\r\n        <img src=https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Bioruptor-Pico-e1676899111358.jpg alt=\"\" class=\"attachment-sote-gallery-big size-sote-gallery-big\" srcset=\"\" >\r\n        <\/a><\/div><\/span><span class=\"gallery-item\"><div class=\"hidden\"><a href=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/TapeStation-4150.jpg\">\r\n        <img src=https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/TapeStation-4150.jpg alt=\"\" class=\"attachment-sote-gallery-big size-sote-gallery-big\" srcset=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/TapeStation-4150-209x300.jpg 209w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/TapeStation-4150.jpg 279w\" >\r\n        <\/a><\/div><\/span><span class=\"gallery-item\"><div class=\"hidden\"><a href=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Fragment_analyzer.jpg\">\r\n        <img src=https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Fragment_analyzer.jpg alt=\"\" class=\"attachment-sote-gallery-big size-sote-gallery-big\" srcset=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Fragment_analyzer-400x300.jpg 400w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Fragment_analyzer-300x225.jpg 300w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Fragment_analyzer.jpg 632w\" >\r\n        <\/a><\/div><\/span><span class=\"gallery-item\"><div class=\"hidden\"><a href=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/QiaCUBE-Connect.jpg\">\r\n        <img src=https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/QiaCUBE-Connect.jpg alt=\"Gy\u00f6ngyi Munk\u00e1csy Dr. \" class=\"attachment-sote-gallery-big size-sote-gallery-big\" srcset=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/QiaCUBE-Connect-400x290.jpg 400w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/QiaCUBE-Connect-300x217.jpg 300w, https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/QiaCUBE-Connect.jpg 753w\" >\r\n        <\/a><\/div><\/span><span class=\"gallery-item\"><div class=\"hidden\"><a href=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Fragment-Analyser-2_kepvalto.jpg\">\r\n        <img src=https:\/\/semmelweis.hu\/corefacility\/files\/2023\/02\/Fragment-Analyser-2_kepvalto.jpg alt=\"\" class=\"attachment-sote-gallery-big size-sote-gallery-big\" srcset=\"\" >\r\n        <\/a><\/div><\/span>\r\n            <\/div>\r\n\r\n            [\/slb_exclude]\r\n\t\n","protected":false},"excerpt":{"rendered":"<p>Head of Core Facility Gy\u00f6ngyi Munk\u00e1csy, PhD Core Facility Address Department of Bioinformatics T\u0171zolt\u00f3 u. 7. 1094 Budapest Contact E-mail to: Gy\u00f6ngyi Munk\u00e1csy, PhD <a href=\"mailto:munkacsy.gyongyi@semmelweis.hu\" target=\"_blank\" rel=\"noopener\">munkacsy.gyongyi@semmelweis.hu<\/a> Staff 4 Quality Control ISO 9001 What we offer The NGS Core Facility offers Next Generation Sequencing Service that combines tried-and-true instrument technologies and tunable output with sequencing capabilities. Users can &hellip;<\/p>\n","protected":false},"author":102026,"featured_media":15551,"parent":14502,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-fullwidth.php","meta":{"_acf_changed":false,"footnotes":""},"categories":[15,18],"tags":[],"class_list":["post-15765","page","type-page","status-publish","has-post-thumbnail","hentry","category-core-facility","category-molecular-biology"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15765","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/users\/102026"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/comments?post=15765"}],"version-history":[{"count":10,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15765\/revisions"}],"predecessor-version":[{"id":16507,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15765\/revisions\/16507"}],"up":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/14502"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media\/15551"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media?parent=15765"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/categories?post=15765"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/tags?post=15765"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}