{"id":15749,"date":"2023-05-08T13:52:11","date_gmt":"2023-05-08T11:52:11","guid":{"rendered":"https:\/\/semmelweis.hu\/corefacility\/?page_id=15749"},"modified":"2024-03-01T13:35:47","modified_gmt":"2024-03-01T12:35:47","slug":"spatial_transcriptomicsand_proteomics","status":"publish","type":"page","link":"https:\/\/semmelweis.hu\/corefacility\/home\/spatial_transcriptomicsand_proteomics\/","title":{"rendered":"Spatial transcriptomics and proteomics"},"content":{"rendered":"<div class=\"keretes\" style=\"border-left: 6px solid #4d9de0 !important\">\n<p><strong>Head of Core Facility<br \/>\n<\/strong>Csaba B\u00f6d\u00f6r, PhD<\/p>\n<p><strong>Core Facility Address<br \/>\n<\/strong>Department of Pathology and Experimental Cancer Research<br \/>\n\u00dcll\u0151i \u00fat 26.<br \/>\n1085 Budapest<\/p>\n<p><strong>Contact E-mail<br \/>\n<\/strong>to: Csaba B\u00f6d\u00f6r, PhD<strong><br \/>\n<\/strong><a href=\"mailto:bodor.csaba1@semmelweis.hu\">bodor.csaba1@semmelweis.hu<\/a><\/p>\n<p><strong>Number of staff<br \/>\n<\/strong>2<\/p>\n<p><strong>Quality Control<br \/>\n<\/strong>ISO 9001<\/p>\n<\/div>\n<h4><strong>What we offer<\/strong><\/h4>\n<p>NanoString GeoMx\u00ae DSP experiment projects with the reagent kits available from the manufacturer.<\/p>\n<ul style=\"list-style-type: square\">\n<li>NanoString GeoMx\u00ae DSP tissue slide preparation and region-of-interest selection for RNA targets<\/li>\n<li>NanoString GeoMx\u00ae DSP tissue slide preparation and region-of-interest selection for protein targets<\/li>\n<li>Readout with the NanoString nCounter system or next-generation sequencing (Illumina MiSeq or NextSeq2000)<\/li>\n<li>Downstream data analysis using the on-board software of the GeoMx\u00ae DSP system<\/li>\n<li>Remote Desktop approaches can be used to instrument the GeoMx platform in order to afford ROI selection and data analysis for external researchers.<\/li>\n<\/ul>\n<h4><strong>Why work with us<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>\n<div>\n<p class=\"x_MsoNormal\">Our spatial transcriptomics and proteomics core facility utilizing <span style=\"font-size: 1rem\">the Nanostring GeoMx\u00ae platform provides an end-to-end solution <\/span><span style=\"font-size: 1rem\">for spatially resolved analyses with downstream next-generation <\/span><span style=\"font-size: 1rem\">sequencing based readouts also available in our laboratory.<\/span><\/p>\n<\/div>\n<\/li>\n<\/ul>\n<h4><strong>equipmentS<\/strong><\/h4>\n<ul>\n<li>NanoString\u2019s GeoMx Digital Spatial Profiler (DSP)<\/li>\n<li class=\"x_MsoNormal\">MiSeq next-generation sequencer (Illumina)<\/li>\n<li class=\"x_MsoNormal\"><span style=\"font-size: 1rem\">NextSeq2000\u00a0<\/span><span class=\"x_contentpasted1\" style=\"font-size: 1rem\">next-generation sequencer (Illumina)<\/span><\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/semmelweis.hu\/corefacility\/files\/2022\/01\/RS139483_C19A1350-scr.jpg\" alt=\"\" width=\"400\" height=\"267\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" style=\"color: #1e2326;font-family: 'PT serif', serif;font-size: 1rem\" src=\"https:\/\/semmelweis.hu\/corefacility\/files\/2022\/01\/RS139491_C19A1369-scr.jpg\" alt=\"\" width=\"400\" height=\"267\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/10\/RS139487_C19A1360-Bodor_Csaba.jpg\" alt=\"\" width=\"402\" height=\"268\" \/> \u00a0<img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/10\/RS139485_C19A1356-Bodor_Csaba.jpg\" width=\"402\" height=\"268\" \/><\/p>\n<h4>\u00a0<\/h4>\n<h4><strong>References<\/strong><\/h4>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube.com\/embed\/DVmxkVykLHU?si=EgBfupwmcTM0oZ0S\" width=\"560\" height=\"315\"><\/iframe><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Head of Core Facility Csaba B\u00f6d\u00f6r, PhD Core Facility Address Department of Pathology and Experimental Cancer Research \u00dcll\u0151i \u00fat 26. 1085 Budapest Contact E-mail to: Csaba B\u00f6d\u00f6r, PhD <a href=\"mailto:bodor.csaba1@semmelweis.hu\">bodor.csaba1@semmelweis.hu<\/a> Number of staff 2 Quality Control ISO 9001 What we offer NanoString GeoMx\u00ae DSP experiment projects with the reagent kits available from the manufacturer. NanoString GeoMx\u00ae DSP &hellip;<\/p>\n","protected":false},"author":102026,"featured_media":15272,"parent":14502,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-fullwidth.php","meta":{"_acf_changed":false,"footnotes":""},"categories":[15,18],"tags":[],"class_list":["post-15749","page","type-page","status-publish","has-post-thumbnail","hentry","category-core-facility","category-molecular-biology"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15749","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/users\/102026"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/comments?post=15749"}],"version-history":[{"count":10,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15749\/revisions"}],"predecessor-version":[{"id":16402,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15749\/revisions\/16402"}],"up":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/14502"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media\/15272"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media?parent=15749"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/categories?post=15749"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/tags?post=15749"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}