{"id":15737,"date":"2023-05-08T13:37:20","date_gmt":"2023-05-08T11:37:20","guid":{"rendered":"https:\/\/semmelweis.hu\/corefacility\/?page_id=15737"},"modified":"2024-03-01T12:10:56","modified_gmt":"2024-03-01T11:10:56","slug":"wes-simple-system-core-facility","status":"publish","type":"page","link":"https:\/\/semmelweis.hu\/corefacility\/home\/wes-simple-system-core-facility\/","title":{"rendered":"Capillary based protein expression analysis"},"content":{"rendered":"<div class=\"keretes\" style=\"border-left: 6px solid #4d9de0 !important\">\n<p><strong>Head of Core Facility<br \/>\n<\/strong>Anna Sebesty\u00e9n, PhD<\/p>\n<p><strong>Core Facility Address<br \/>\n<\/strong>Department of Pathology and Experimental Cancer Research<br \/>\n\u00dcll\u0151i \u00fat 26.<br \/>\n1085 Budapest<\/p>\n<p><strong>Contact<br \/>\n<\/strong>to: Anna Sebesty\u00e9n, PhD<strong><br \/>\n<\/strong><a href=\"mailto:sebestyen.anna@semmelweis.hu\" target=\"_blank\" rel=\"noopener\">sebestyen.anna@semmelweis.hu<\/a><\/p>\n<p><strong>Number of staff<br \/>\n<\/strong>4<\/p>\n<p><strong>Quality Control<br \/>\n<\/strong>ISO 9001<\/p>\n<\/div>\n<h4><strong>What we offer<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>Performing capillary based protein expression analysis<\/li>\n<li>Performing analyses with already established antibodies<\/li>\n<li>Establishing antibodies for new analyses and new tests<\/li>\n<li>Performing final evaluation and data presentation after WES Simple analyses<\/li>\n<li>Analysing the expression of signalling molecules, oncoproteins, metabolic enzymes, transcription factors, membrane bounded markers<\/li>\n<\/ul>\n<p>Tumour Biology Laboratory in Department of Pathology and Experimental Cancer Research to analyse protein expression pattern from small sample sizes of different cell and tissue lysates in research experiments. This analytical methods has several advantages (time consuming, automated, low inter- and intra assays CVs in absolute and relative protein quantification, low sample size is required, high sensitivity).<\/p>\n<h4><strong>Why work with us<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>The Tumour Biology Laboratory at Department of Pathology and Experimental Cancer Research Institute has wide experience studying protein expression changes related to tumorigenic alterations in different samples including fresh and frozen cell and tissue materials<\/li>\n<li>Simple\u00a0<span class=\"marky1n6xic8f\" data-markjs=\"true\" data-ogac=\"\" data-ogab=\"\" data-ogsc=\"\" data-ogsb=\"\">Wes<\/span>tern offers fully automate traditional <span class=\"marky1n6xic8f\" data-markjs=\"true\" data-ogac=\"\" data-ogab=\"\" data-ogsc=\"\" data-ogsb=\"\">wes<\/span>tern blotting with quantitative evaluation<\/li>\n<li>This method is applicable in quantitative analyses simultaneously more proteins in small sample size regarding only ~1000 cells<\/li>\n<li>WES Simple System is providing data on 24 samples in only 3 hours. Using this capillary based technology protein expression and total protein amount can be analysed by size and immunoassay<\/li>\n<\/ul>\n<h4><strong>equipment<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>WES Simple System<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/semmelweis.hu\/corefacility\/files\/2022\/01\/WES2-scaled.jpg\" alt=\"\" width=\"350\" height=\"263\" \/>\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/semmelweis.hu\/corefacility\/files\/2022\/01\/WES-Capillary-scaled.jpg\" alt=\"\" width=\"200\" height=\"266\" \/><\/li>\n<\/ul>\n<h4><strong>References<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/pmc9503093\/\" target=\"_blank\" rel=\"noopener\">http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/pmc9503093\/<\/a><\/li>\n<li><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/pmc9098092\/\" target=\"_blank\" rel=\"noopener\">http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/pmc9098092\/<\/a><\/li>\n<li>\n<p><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/pmc8439280\/\" target=\"_blank\" rel=\"noopener\">http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/pmc8439280\/<\/a><\/p>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Head of Core Facility Anna Sebesty\u00e9n, PhD Core Facility Address Department of Pathology and Experimental Cancer Research \u00dcll\u0151i \u00fat 26. 1085 Budapest Contact to: Anna Sebesty\u00e9n, PhD <a href=\"mailto:sebestyen.anna@semmelweis.hu\" target=\"_blank\" rel=\"noopener\">sebestyen.anna@semmelweis.hu<\/a> Number of staff 4 Quality Control ISO 9001 What we offer Performing capillary based protein expression analysis Performing analyses with already established antibodies Establishing antibodies for new analyses &hellip;<\/p>\n","protected":false},"author":102026,"featured_media":15254,"parent":14502,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-fullwidth.php","meta":{"_acf_changed":false,"footnotes":""},"categories":[15,18],"tags":[33],"class_list":["post-15737","page","type-page","status-publish","has-post-thumbnail","hentry","category-core-facility","category-molecular-biology","tag-analysis"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15737","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/users\/102026"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/comments?post=15737"}],"version-history":[{"count":10,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15737\/revisions"}],"predecessor-version":[{"id":16399,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15737\/revisions\/16399"}],"up":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/14502"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media\/15254"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media?parent=15737"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/categories?post=15737"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/tags?post=15737"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}