{"id":15676,"date":"2023-04-04T12:33:27","date_gmt":"2023-04-04T10:33:27","guid":{"rendered":"https:\/\/semmelweis.hu\/corefacility\/?page_id=15676"},"modified":"2024-11-07T15:26:08","modified_gmt":"2024-11-07T14:26:08","slug":"facs-core-facility-ii","status":"publish","type":"page","link":"https:\/\/semmelweis.hu\/corefacility\/home\/facs-core-facility-ii\/","title":{"rendered":"FACS Core Facility in EOK"},"content":{"rendered":"<div class=\"keretes\" style=\"border-left: 6px solid #4d9de0 !important\">\n<p><strong>Head of Core Facility<br \/>\n<\/strong>Karolina Pircs, PhD<\/p>\n<p><strong>Core Facility Address<br \/>\n<\/strong>HCEMM-SU Neurobiology and neurodegenerative diseases Research Group<br \/>\nEOK, Basic Medical Science Center<br \/>\n0.116 laboratory room<br \/>\nD ground floor<\/p>\n<p>Institute of Translational Medicine<br \/>\nSemmelweis University<br \/>\nT\u0171zolt\u00f3 street. 37\u201347.<br \/>\n1094 Budapest<\/p>\n<p><strong>Contact<br \/>\n<\/strong>to: \u00c1gnes Varga, PhD<br \/>\n<a href=\"mailto:varga.agnes1@semmelweis.hu\">varga.agnes1@semmelweis.hu<\/a><br \/>\nMobile: +36 30 016 4787<\/p>\n<p>cc: Karolina Pircs, PhD<br \/>\n<a href=\"mailto:pircs.karolina@semmelweis.hu\">pircs.karolina@semmelweis.hu<\/a><\/p>\n<p><strong>Number of staff<br \/>\n<\/strong>4 persons<\/p>\n<p><strong>Quality Control<br \/>\n<\/strong>ISO 9001\u00a0<\/p>\n<\/div>\n<h4><strong>What we offer<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>Access to state-of-the art flow cytometer and cell sorting services.<\/li>\n<li>Complex flow cytometry analysis of cell populations even with 15 fluorescent channels<\/li>\n<li>Sorting of rare populations out of a heterogeneous sample. Cell enrichment, and high purity sorting<\/li>\n<li>Indexed sorting for single-cell analysis<\/li>\n<li>Longstanding expertise, help in experimental design and data analysis<\/li>\n<li>Quick, easy booking system, where sorting services are provided with assistance within 2 weeks after permitted applications<\/li>\n<li>Services are available to all internal and external academic customers (international customers please contact us for pricing). Semmelweis University users have priority access<\/li>\n<\/ul>\n<p><strong style=\"color: #313b40;font-family: Montserrat, sans-serif;font-size: 22px;text-transform: uppercase\">Why work with us<\/strong><\/p>\n<ul style=\"list-style-type: square\">\n<li>State-of-the-art FACS sorting service for SU researchers uniquily in the EOK building<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h4><strong>equipment<\/strong><\/h4>\n<p><a href=\"https:\/\/semmelweis.hu\/corefacility\/facility\/facs-core-facility\/flow-cytometry-cytoflex-srt-with-pump-cart-600x400-1-400x134\/\" rel=\"attachment wp-att-15448\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-15448 size-medium\" src=\"https:\/\/semmelweis.hu\/corefacility\/files\/2022\/12\/Flow-Cytometry-CytoFLEX-SRT-with-pump-cart-600x400-1-400x134-1-300x101.jpeg\" alt=\"Beckman Coulter CytoFLEX SRT\" width=\"300\" height=\"101\" srcset=\"https:\/\/semmelweis.hu\/corefacility\/files\/2022\/12\/Flow-Cytometry-CytoFLEX-SRT-with-pump-cart-600x400-1-400x134-1-300x101.jpeg 300w, https:\/\/semmelweis.hu\/corefacility\/files\/2022\/12\/Flow-Cytometry-CytoFLEX-SRT-with-pump-cart-600x400-1-400x134-1.jpeg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><strong>Beckman Coulter CytoFLEX SRT cell sorter<\/strong> is equipped with 4 lasers (violet at 405, blue at 488, yellow at 561 and red at 638 nm) and 17 detectors to measure up to 15 fluorescent channels and two scatter parameters. It facilitates to design experiments with complex sort logic. The machine is capable to sort simultaneously 4 defined cell populations. The system supports 5 ml (12\u00d775 mm) tubes or Eppendorf tubes as input. The following sorting devices are supported: 5 mL, 15 ml tubes, different plates up to 384-well and slides. Additionally, the machine is able to perform index sorting, which allows single cells sorts into plates or slides for single cell genomic or transcriptomic analyses. There is also an option to collect side scatter signal from Violet (405 nm) laser. The configured channel (VSSC) can be utilized to better resolve nanoparticles. Analyzation of the data can be done individually with the help of CytExpert SRT program on any computer.<\/p>\n<p>&nbsp;<\/p>\n<h4><strong>References<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li style=\"list-style-type: none\">\n<ul style=\"list-style-type: square\">\n<li>\u00a0<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Head of Core Facility Karolina Pircs, PhD Core Facility Address HCEMM-SU Neurobiology and neurodegenerative diseases Research Group EOK, Basic Medical Science Center 0.116 laboratory room D ground floor Institute of Translational Medicine Semmelweis University T\u0171zolt\u00f3 street. 37\u201347. 1094 Budapest Contact to: \u00c1gnes Varga, PhD <a href=\"mailto:varga.agnes1@semmelweis.hu\">varga.agnes1@semmelweis.hu<\/a> Mobile: +36 30 016 4787 cc: Karolina Pircs, PhD <a href=\"mailto:pircs.karolina@semmelweis.hu\">pircs.karolina@semmelweis.hu<\/a> Number of &hellip;<\/p>\n","protected":false},"author":102026,"featured_media":15467,"parent":14502,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-fullwidth.php","meta":{"_acf_changed":false,"footnotes":""},"categories":[15,18],"tags":[],"class_list":["post-15676","page","type-page","status-publish","has-post-thumbnail","hentry","category-core-facility","category-molecular-biology"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15676","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/users\/102026"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/comments?post=15676"}],"version-history":[{"count":10,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15676\/revisions"}],"predecessor-version":[{"id":16505,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15676\/revisions\/16505"}],"up":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/14502"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media\/15467"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media?parent=15676"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/categories?post=15676"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/tags?post=15676"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}