{"id":15669,"date":"2023-04-04T12:32:31","date_gmt":"2023-04-04T10:32:31","guid":{"rendered":"https:\/\/semmelweis.hu\/corefacility\/?page_id=15669"},"modified":"2024-11-07T15:28:24","modified_gmt":"2024-11-07T14:28:24","slug":"facs-core-facility-i","status":"publish","type":"page","link":"https:\/\/semmelweis.hu\/corefacility\/home\/facs-core-facility-i\/","title":{"rendered":"FACS Core Facility in NET"},"content":{"rendered":"<div class=\"keretes\" style=\"border-left: 6px solid #4d9de0 !important\">\n<p><strong>Head of Core Facility<br \/>\n<\/strong>Prof. Edit Buz\u00e1s, MD, PhD<\/p>\n<p><strong>Core Facility Address<br \/>\n<\/strong>Department of Genetics, Cell and Immunobiology<br \/>\nNET 622\/A room 6th floor<br \/>\nNagyv\u00e1rad square 4.<br \/>\n1089 Budapest,<\/p>\n<p><strong>Contact<br \/>\n<\/strong>to: T\u00fcnde B\u00e1rkai<br \/>\n<a href=\"mailto:barkai.tunde@semmelweis.hu\">barkai.tunde@semmelweis.hu<\/a><br \/>\nProf. Edit Buz\u00e1s, MD, PhD<br \/>\n<a href=\"mailto:buzas.edit@semmelweis.hu\">buzas.edit@semmelweis.hu<\/a><\/p>\n<p><strong>Number of staff<br \/>\n<\/strong>4 persons<\/p>\n<p><strong>Quality Control<br \/>\n<\/strong>ISO 9001<\/p>\n<\/div>\n<h4><strong>What we offer<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>Access to state-of-the art flow cytometer and cell sorting services<\/li>\n<li>Complex flow cytometry analysis of cell populations even with 15 fluorescent channels<\/li>\n<li>Sorting of rare populations out of a heterogeneous sample. Cell enrichment, and high purity sorting<\/li>\n<li>Indexed sorting for single-cell analysis<\/li>\n<li>Longstanding expertise, help in experimental design and data analysis<\/li>\n<li>Quick, easy booking system, where sorting services are provided with assistance within 2 weeks after permitted applications<\/li>\n<li>Services are available to all internal and external academic customers (international customers please contact us for pricing). Semmelweis University users have priority access<\/li>\n<\/ul>\n<h4><strong>Why work with us<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>State-of-the-art FACS sorting service for SU researchers uniquily in the NET building<\/li>\n<\/ul>\n<h4><strong>equipment<\/strong><\/h4>\n<p><a href=\"https:\/\/semmelweis.hu\/corefacility\/facility\/facs-core-facility\/sony-sorter-753x565\/\" rel=\"attachment wp-att-15449\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-15449 alignleft\" src=\"https:\/\/semmelweis.hu\/corefacility\/files\/2022\/12\/Sony-sorter-753x565-1-300x225.jpeg\" alt=\"\" width=\"267\" height=\"200\" srcset=\"https:\/\/semmelweis.hu\/corefacility\/files\/2022\/12\/Sony-sorter-753x565-1-300x225.jpeg 300w, https:\/\/semmelweis.hu\/corefacility\/files\/2022\/12\/Sony-sorter-753x565-1-400x300.jpeg 400w, https:\/\/semmelweis.hu\/corefacility\/files\/2022\/12\/Sony-sorter-753x565-1.jpeg 753w\" sizes=\"auto, (max-width: 267px) 100vw, 267px\" \/><\/a><strong>Sony LE- SH800SFP Fluorescence-based sorting<\/strong> is available on a new generation chip-based Sony sorter with 4 lasers (488 nm, 405 nm, 638 nm\u00a0and 561 nm), two scatter and six fluorescence parameters. The sorter is able to work with a wide range of fluorochromes, <span style=\"font-size: 14pt\">fluorescent<\/span> proteins and vitality or cell tracker stains thanks to its 4 lasers and versatile filter systems (max 6 channels). The machine can perform two-way sorting in flow cytometry tubes, or in 8 well strips, microscopic slides or 6-,12-, 24-, 48-, 96- and 384-well plates. <br \/>\n<span style=\"font-size: 15pt\"><br \/>\nThe theoretical and optical background of chip-based sorting is very different from conventional sorting, so please consult our staff before designing any panel. Thanks to the low sheet pressure, sensitive cell types can be sorted for further culturing.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Head of Core Facility Prof. Edit Buz\u00e1s, MD, PhD Core Facility Address Department of Genetics, Cell and Immunobiology NET 622\/A room 6th floor Nagyv\u00e1rad square 4. 1089 Budapest, Contact to: T\u00fcnde B\u00e1rkai <a href=\"mailto:barkai.tunde@semmelweis.hu\">barkai.tunde@semmelweis.hu<\/a> Prof. Edit Buz\u00e1s, MD, PhD <a href=\"mailto:buzas.edit@semmelweis.hu\">buzas.edit@semmelweis.hu<\/a> Number of staff 4 persons Quality Control ISO 9001 What we offer Access to state-of-the art flow cytometer &hellip;<\/p>\n","protected":false},"author":102026,"featured_media":15449,"parent":14502,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-fullwidth.php","meta":{"_acf_changed":false,"footnotes":""},"categories":[15,18],"tags":[],"class_list":["post-15669","page","type-page","status-publish","has-post-thumbnail","hentry","category-core-facility","category-molecular-biology"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15669","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/users\/102026"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/comments?post=15669"}],"version-history":[{"count":10,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15669\/revisions"}],"predecessor-version":[{"id":16506,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15669\/revisions\/16506"}],"up":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/14502"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media\/15449"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media?parent=15669"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/categories?post=15669"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/tags?post=15669"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}