{"id":15630,"date":"2023-04-27T11:09:19","date_gmt":"2023-04-27T09:09:19","guid":{"rendered":"https:\/\/semmelweis.hu\/corefacility\/?page_id=15630"},"modified":"2024-06-12T11:54:30","modified_gmt":"2024-06-12T09:54:30","slug":"experimental-advanced-histopathology","status":"publish","type":"page","link":"https:\/\/semmelweis.hu\/corefacility\/home\/experimental-advanced-histopathology\/","title":{"rendered":"Experimental Advanced Histopathology"},"content":{"rendered":"<div class=\"keretes\" style=\"border-left: 6px solid #4d9de0 !important\">\n<p><strong>Head of Core Facility<br \/>\n<\/strong>Zolt\u00e1n Varga PhD<\/p>\n<p><strong>Core Facility Address<br \/>\n<\/strong>Department of Pharmacology and Pharmacotherapy<br \/>\nNagyvarad t\u00e9r 4. <br \/>\n5th floor, room 511<br \/>\nH-1089 Budapest<\/p>\n<p><strong>Contact e-mail<br \/>\n<\/strong>to: Zolt\u00e1n Varga PhD<br \/>\n<a href=\"mailto:varga.zoltan@med.semmelweis-univ.hu\">varga.zoltan@med.semmelweis-univ.hu<\/a><\/p>\n<p><strong>Number of staff<br \/>\n<\/strong>4<\/p>\n<p><strong>Quality Control<br \/>\n<\/strong>ISO 9001<\/p>\n<\/div>\n<h4><strong>What we offer<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>Routine tissue processing<\/li>\n<li>Embedding and sectioning <br \/>\n(FFPE, fresh frozen)<\/li>\n<li>Deparaffinization and routine stainings <br \/>\n(H&amp;E, Masson\u2019s trichome, Sirius Red, PAS, OilRed-O)<\/li>\n<li>Microscopy-documentation analysis<\/li>\n<li>Immunohistochemistry\n<ul style=\"list-style-type: circle\">\n<li>Oxidative\/nitrative stress <br \/>\n(4-HNE, 3-NT)<\/li>\n<li>Vascular adhesion molecules <br \/>\n(E-Sele, V-CAM, I-CAM)<\/li>\n<li>Immune cells<br \/>\n(Iba1, CD68,\u00a0Ly6g, MPO,\u00a0CD3, CD4, CD8, CD45R \u2013 B220)<\/li>\n<\/ul>\n<\/li>\n<li>RNAScope in situ hybridization<\/li>\n<li>Laser capture microdissection<\/li>\n<\/ul>\n<h4>\u00a0<\/h4>\n<h4><strong>Why work with us<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>We make 2D histology into clear interpretable data.<\/li>\n<li>We are specialized in 2D histology using automated quantification of disease markers and therapeutic endpoints.<\/li>\n<li>We offer the full-range of histology services to ensure efficient, complete and high-quality evaluation of study endpoints<\/li>\n<\/ul>\n<p><strong>Further benefits:<\/strong><\/p>\n<ul style=\"list-style-type: square\">\n<li>Expert histology solutions<\/li>\n<li>Clinical endpoints to ensure translatability<\/li>\n<li>Histopathology hallmark stainings<\/li>\n<li>High\u2010throughput multiplex stainings<\/li>\n<li>Spatial gene expression solutions<\/li>\n<\/ul>\n<h4>\u00a0<\/h4>\n<h4><strong>equipmentS<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>LEICA LED DM 3000 Microscope<\/li>\n<li>LEICA SP8 confocal microscope<\/li>\n<li>LEICA CM1950 Cryostat<\/li>\n<li>LEICA LMD6 laser microdissection system<\/li>\n<li>LEICA TP1020 Semi-enclosed Tissue Processor with vacuum <br \/>\nand carbon filters (for formaldehyde, and for organics, Xylene standard)<\/li>\n<li>LEICA Arcadia H- Heated Paraffin Embedding Station<\/li>\n<li>LEICA Arcadia C- Cold plate with poxer cord<\/li>\n<li>LEICA EG F Electric, Heatable Forceps<\/li>\n<li>LEICA RM2245 Semi-Automated Rotary Microtome<\/li>\n<li>LEICA HI1210 Water bath for paraffin sections<\/li>\n<li>LEICA HI1220 Flattening table<\/li>\n<\/ul>\n<h4>\u00a0<\/h4>\n<h4><strong>References<\/strong><\/h4>\n<ul style=\"list-style-type: square\">\n<li>\u00a0<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n[slb_exclude]<div data-carousel-extra='{\"blog_id\":194,\"permalink\":\"https:\\\/\\\/semmelweis.hu\\\/corefacility\\\/home\\\/experimental-advanced-histopathology\\\/\"}' class=\"tiled-gallery type-rectangular\" data-original-width=\"753\"><div class=\"gallery-row\" style=\"width: 748px; 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height: 558px;\"><div class=\"gallery-group images-1\" style=\"width: 748px; height: 562px;\"><div class=\"tiled-gallery-item tiled-gallery-item-large\"><a href=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/04\/3_Varga_Zoltan_kep.jpg\"><img loading=\"lazy\" decoding=\"async\" data-attachment-id=\"15641\" data-orig-file=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/04\/3_Varga_Zoltan_kep.jpg\" data-orig-size=\"1200,900\" data-comments-opened=\"0\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;1,0,1158,3UnProcessed&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;1&quot;}\" data-image-title=\"3_Varga_Zoltan_kep\" data-image-description=\"\" data-medium-file=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/04\/3_Varga_Zoltan_kep-400x300.jpg\" data-large-file=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/04\/3_Varga_Zoltan_kep-1024x768.jpg\" src=\"https:\/\/semmelweis.hu\/corefacility\/files\/2023\/04\/3_Varga_Zoltan_kep-744x558.jpg\" width=\"744\" height=\"558\" align=\"left\" title=\"1,0,1158,3UnProcessed\" \/><\/a><div class=\"tiled-gallery-caption\">1,0,1158,3UnProcessed<\/div><\/div><\/div><\/div><\/div>[\/slb_exclude]\n","protected":false},"excerpt":{"rendered":"<p>Head of Core Facility Zolt\u00e1n Varga PhD Core Facility Address Department of Pharmacology and Pharmacotherapy Nagyvarad t\u00e9r 4. 5th floor, room 511 H-1089 Budapest Contact e-mail to: Zolt\u00e1n Varga PhD <a href=\"mailto:varga.zoltan@med.semmelweis-univ.hu\">varga.zoltan@med.semmelweis-univ.hu<\/a> Number of staff 4 Quality Control ISO 9001 What we offer Routine tissue processing Embedding and sectioning (FFPE, fresh frozen) Deparaffinization and routine stainings (H&amp;E, &hellip;<\/p>\n","protected":false},"author":102026,"featured_media":15299,"parent":14502,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-fullwidth.php","meta":{"_acf_changed":false,"footnotes":""},"categories":[15,17],"tags":[],"class_list":["post-15630","page","type-page","status-publish","has-post-thumbnail","hentry","category-core-facility","category-preclinical-research"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15630","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/users\/102026"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/comments?post=15630"}],"version-history":[{"count":11,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15630\/revisions"}],"predecessor-version":[{"id":16032,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/15630\/revisions\/16032"}],"up":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/pages\/14502"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media\/15299"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/media?parent=15630"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/categories?post=15630"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/corefacility\/wp-json\/wp\/v2\/tags?post=15630"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}