We are primarity interested in the role of mitochondria in cancer; alterations in oncometabolism substantiated by changes in proteins expression ‘rewiring’ certain metabolic pathways provide an excellent opportunity for cancer-specific therapeutic intervention. Identifying those proteins involved in bioenergetic pathways that are up- or downregulated in order to serve the needs of neoplasia, is crucial for beating cancer.
One way for addressing is this by applying Reverse Phase Protein Array technology, specifically designed for mitochondrial proteins. For more information see http://rppa.hu. Please note that clicking this page leads outside of semmelweis.hu addresses.
Furthermore, we also collaborate with many institutes around the globe on disease models exhibiting mitochondrial involvement such as Charcot-Marie-Tooth (CMT) disease, Leber’s hereditary optic neuropathy (LHON), methylmalonyl acidemias, mtDNA depletion syndromes, etc.
We are also interested in the cell-specific expression of mitochondrial proteins in the human brain, see figure below:
OGDH immunolabeling in the human cerebral cortex in relation to neuronal and glial markers. a OGDH isoform 1/2 (green) immunolabeling and fluorescent Nissl staining (red) show many double-labeled yellow cells in the cerebral cortex. b A high magnification confocal image of a cerebral cortical section double stained with OGDH isoform 1/2 (green) and fluorescent Nissl staining (red) demonstrates that larger, neuronal cells indicated with white arrow are double labeled. Several small cells are also present, which are not labeled with OGDH isoform 1/2. Note the dot-like intracellular labeling pattern of OGDH subunit 1/2 immunoreactivity. c A cerebral cortical section double labeled with OGDH isoform 1/2 (green) and the established astrocyte marker glial fibrillary acidic protein (GFAP; red) shows only single-labeled cells positive for OGDH isoform 1/2 or for GFAP, demonstrating that astrocytes are not labeled with OGDH isoform 1. d A high magnification confocal image shows separate single OGDH isoform 1/2 and GFAP-positive (red, pointed to by arrowheads) cells.
Finally, we are investigating the possibility that human intelligence depends on the expression of specific mitochondrial proteins such as ARHGAP11B, see this paper.
ANT activity research assay page – the page contains many links that lead outside of semmelweis.hu addresses. Please use the page at your own risk.
Please contact Dr. Christos Chinopoulos MD, PhD for more information.