Lectures
Day | Time | Location |
Wednesdays | 11:10-12:20 | Szent-Györgyi Albert lecture hall |
Thursdays | 12:35-13:45 | Szent-Györgyi Albert lecture hall |
Semmelweis University, EOK B uilding Budapest IX., Tűzoltó utca 37-47 H-1094
Topic | # of lectures | Lecturer | |
Amino acids, proteins, enzymes | 8 | Prof. Sasvári, Mária | MS |
Bioenergetics-I | 3 | Dr. Chinopoulos, Christos | CC |
Subcellular biochemistry | 3 | Dr. Csala, Miklós | MCs |
Molecular biology | 6 | Dr. Bauer, Pál | PB |
5 | Dr. Törőcsik, Beáta | TB | |
3 | Prof. Tretter, László | LT |
Week | Topic | |||
01 | 03 | Feb | Amino acids. Amino acids as electrolytes. Structure and chirality of amino acids. Reactions of amino acids (MS) | |
01 | 04 | Feb | Proteins. The peptide bond. Structure levels in proteins. Primary structure of proteins (MS) | |
02 | 10 | Feb | Steric structure of globular proteins. Conformation of proteins. Purification of proteins (MS) |
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02 | 11 | Feb | Structural characteristics of fibrous proteins. Collagen (MS) | |
03 | 17 | Feb | Myoglobin and hemoglobin (MS) | |
03 | 18 | Feb | Enzymes. Enzymes as biocatalysts, enzyme activity. Isoenzymes. Coenzymes (MS) | |
04 | 24 | Feb | Enzyme kinetics: the Michaelis-Menten model. Mechanism of action of some important enzymes (serine proteases) (MS) | |
04 | 25 | Feb | Reversible and irreversible inhibition of enzymes; competitive, non-competitive and uncompetitive inhibitors. Regulation of enzyme activity. Allosteric enzymes (MS) | |
05 | 02 | Mar | Principles of bioenergetics: energy transformation, group transfer potential, coupled reactions, substrate level phosphorylation (CC) | |
05 | 03 | Mar | Role of mitochondria in biological oxidation. Terminal oxidation (CC) | |
06 | 09 | Mar | Oxidative phosphorylation (CC) | |
06 | 10 | Mar | Compartmentation in eukaryotic cells. Membrane structure. Intracellular membranes and organelles. Structure and function of the nucleus (MCs) | |
07 | 16 | Mar | Movement of cellular organelles. Cytoskeleton, microfilaments, microtubuli,actomyosin. Mechanism of vesicular transport (MCs) | |
07 | 17 | Mar | Metabolism and transport, the principle of metabolom. Metabolic profile of various organelles (endoplasmic reticulum, peroxisomes, lysosomes, mitochondria) (MCs) | |
Springbreak, Easter Monday (21-28 III) | ||||
08 | 30 | Mar | Nucleic acids – structure and function. Bases, nucleosides, nucleotides, DNA structure, DNA denaturation, hybridization (PB) | |
08 | 31 | Mar | DNA replication. Replication in prokaryotes, leading and lagging strand, Okazaki fragments. DNA-dependent DNA polymerases. DNA ligase. Telomerase and topoizomerases. Replication in eukaryotes. Structure of eukaryotic chromosomes. Mitochondrial DNA. Nucleosome structure (PB) |
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09 | 06 | Apr | DNA repair. Types of DNA damage; mutations, frameshift, nonsense mutations, mismatch repair. Coordination of repair and replication (TB) |
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09 | 07 | Apr | Transcription in prokaryotes. Structure of RNA; t-RNA, r-RNA, m-RNA, differences between prokaryotic and eukaryotic genomes. Transcription complexes, initiation, elongation, termination in prokaryotes (PB) | |
10 | 13 | Apr | Transcription in eukaryotes, RNA polymerases, promoters, enhancers, silencers. Processing of mRNA, mechanism of splicing. Alternative splicing. mRNA editing (PB) |
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10 | 14 | Apr | The genetic code. Activation of tRNA. Mechanism of translation: initiation, elongation, termination. Antibiotics. Posttranslational modifications of proteins (PB) |
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11 | 20 | Apr | Protein folding, sorting, quality control and transport into intracellular compartments. Ubiquitination and intracellular proteolysis (PB) | |
11 | 21 | Apr | Regulation of gene expression in prokaryotes. The operon model; positive and negative regulation in the lac operon (LT) | |
12 | 27 | Apr | Regulation of gene expression at the transcriptional level in eukaryotes. Role of chromatine structure; covalent and non-covalent chromatin modifying activities and DNA methylation (epigenetics) (LT) | |
12 | 28 | Apr | Post-transcriptional regulation of gene expression in eukaryotes. Regulation of mRNA stability; microRNAs. Translational control (LT) | |
13 | 04 | May | Cell cycle in eukaryotes. Cyclins and cyclin dependent protein kinases. Proteases in the cell cycle. Regulation of G0/G1, G1/S and G2/M transitions. Integration of DNA repair into the cell cycle (BT) | |
13 | 05 | May | Principles of recombinant DNA technology: molecular cloning, restriction endonucleases. Genomic and cDNA libraries. Blotting techniques (Southern, Northern, Western) and their utilization. DNA microarrays (BT) | |
14 | 11 | May | PCR, real-time PCR and their application in molecular biology. Recombinant vectors (reporter and expression vectors); synthesis of recombinant proteins. Transgenic, knock-out and knock-in animals in medical research. Human gene therapy (BT) | |
14 | 12 | May | The Human Genome Project and its results: organization and polymorphic nature of the human genome; implications for human traits and diseases. Genotyping assays (PCR-RFLP, PCR-ASA). Application of bioinformatics in biological and medical research (BT) |
Lab. lessons
Week | DD-DD | MMM | Laboratory lesson / seminar |
01 | 01-05 | Feb | Carbohydrates (seminar) |
02 | 08-12 | Feb | Precipitation and quantitative determination of proteins. Colour reaction of proteins (lab) |
03 | 15-19 | Feb | Electrometric titration of amino acids (lab) |
04 | 22-26 | Feb | Calculations: amino acids as buffers, protein purification and enzymology (consultation) |
05 | 29/05 | Feb/Mar | Experimental determination of the kinetic parameters of urease (lab) |
06 | 07-11 | Mar | Midterm examination I (topics: amino acids, proteins, enzymes, bioenergetics) |
07 | 16-18 | Mar | Lipids (seminar) |
21-28 | Mar | Springbreak. Easter Monday | |
08 | 29/01 | Mar/Apr | Gel filtration (lab) |
09 | 04-08 | Apr | SDS-PAGE gel electrophoresis and western blot* (lab) |
10 | 11-15 | Apr | Paper and thin layer chromatography* (lab) |
11 | 18-22 | Apr | Induction of ß-galactosidase (lab) |
12 | 25-29 | Apr | Molecular Biology (seminar) |
13 | 02-06 | May | Midterm examination II (topics: molecular biology) |
14 | 09-13 | May | Laboratory exam (written, 15 min). RRestriction digestion of pGL3 basic vector and separation of DNA fragments electrophoresis(lab) |
Schedule of laboratory practical lessons, consultations and seminars
Groups
Group | Day | Time | Laboratory/seminar teacher |
ED1 | Wednesday | 15.10-17.40 | Nagy, Szilvia |
ED2 | Wednesday | 08.00-10.30 | Nagy, Szilvia |
ED3 | Wednesday | 08.00-10.30 | Végh, Miklós |
ED4 | Wednesday | 08.00-10.30 | Vereczkei, Andrea |
EM01 | Monday | 11.4014.40 | Sasvári, Mária |
EM02 | Wednesday | 12.35-15.35 | Bauer, Pál |
EM03 | Monday | 11.4014.40 | Rónai, Zsolt |
EM04 | Tuesday | 15.30-18.30 | Sipeki, Szabolcs |
EM05 | Monday | 15.00-18.00 | Csanády, László |
EM06 | Wednesday | 14.30-17.30 | Spasokoukotskaya, Tatiana |
EM07 | Monday | 11.4014.40 | Bak, Judit |
EM08 | Tuesday | 15.30-18.30 | Margittai, Éva |
EM09 | Monday | 11.4014.40 | Csala, Miklós |
EM10 | Wednesday | 14.30-17.30 | Ambrus, Attila |
EM11 | Monday | 11.4014.40 | Sorum, Ben |
EM12 | Tuesday | 15.30-18.30 | Dóczi, Judit |
EM13 | Tuesday | 14.00-17.00 | Barta, Csaba |
EM14 | Monday | 15.00-18.00 | Sorum, Ben |
EM15 | Monday | 15.00-18.00 | Stroe, Márta |
Venue: Student laboratories of the Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, EOK Building, 1094 Budapest, Tűzoltó u. 37-47., 1st Floor, Passage „D”
Midterm 1
Midterm 2
Topic list
Requirements
Subject
The principal aim of the course is to provide an insight into the structure and function of biologically important molecules including amino acids, carbohydrates, lipids, vitamins, coenzymes (descriptive biochemistry), as well as proteins, enzymes and nucleic acids. This module is a prerequisite to the understanding of the intermediary metabolism of the cell. The cell biology unit describes the principles of organization of cells as well as the function of subcellular organelles. The molecular biology module aims at highlighting the storage and expression of genetic information throughout replication, transcription and translation as well as our current understanding of the regulation of gene expression, followed by an up-to-date summary of currently used methods in molecular biotechnology.
Exemption
- Exemption may be given only if students pass an exemption examination
- Prerequisite of participating in the exemption exam: previous studies and successful examinations in the subject Biochemistry (final exam) (Biochemistry I, II, III)
- Documents to be attached on the previous studies:
a transcript from the former University, containing the name of courses taken in Biochemistry, examination grades and a copy of your final BSc or MSc diploma (if you have)
a topic list of Biochemistry (bioenergetic, metabolism of carbohydrates, lipids, amino acids, nucleotides, biochemistry of tissues, neurobiochemistry, signal transduction, haemostasis and/or molecular biology (macromolecules) (for final exam) (Only official hardcopy version of documentation is accepted, we do not accept e-documentation).
the indication of the used textbook(s) is also recommended
will be organized by our examination committee.
- Oral examinations in Biochemistry
- The exemption examinations take place on the 5th or 6th week of the semester (Febr 29-04 or March 7-11, 2016); the deadline to present your documents is the end of the 2nd week (February 12, 2016).
- In case of unsuccessful exemption examination there are not any negative consequences. You have to attend laboratory lessons and seminars throughout the semester and sit for the regular examination at the end of the semester like the rest of students.
dr. Gergely Keszler |
dr. István Léránt |
teaching secretary of 1st year program in Chemistry and Biochemistry I. |
teaching secretary of 2nd year program in Biochemistry II-III |