{"id":1265,"date":"2014-06-07T21:22:46","date_gmt":"2014-06-07T19:22:46","guid":{"rendered":"http:\/\/www.ana.usn.hu\/hu\/?page_id=1265"},"modified":"2020-04-06T17:31:58","modified_gmt":"2020-04-06T15:31:58","slug":"glia-lab","status":"publish","type":"page","link":"https:\/\/semmelweis.hu\/anatomia\/kutatas\/laborok-kutatocsoportok\/glia-lab\/","title":{"rendered":"Glia Laborat\u00f3rium \/ Glia Laboratory"},"content":{"rendered":"<p><strong>Vezet\u0151 \/ HEAD OF THE LAB:<\/strong><\/p>\n<ul>\n<li>Dr. K\u00e1lm\u00e1n Mih\u00e1ly\u00a0 &#8211; egyetemi tan\u00e1r, az MTA doktora \/\u00a0Ph.D., D.Sc., full professor<\/li>\n<\/ul>\n<p><strong>Munkat\u00e1rsak \/ STAFF:<\/strong><\/p>\n<ul>\n<li>Seb\u0151k Oliv\u00e9r Marcell &#8211; tudom\u00e1nyos seg\u00e9dmunkat\u00e1rs \/ assistant research fellow<\/li>\n<li>\u0150z Andrea -asszisztens \/ technician<\/li>\n<\/ul>\n<div class=\"responsive-tabs\">\n<h2 class=\"tabtitle\">HUNGARIAN<\/h2>\n<div class=\"tabcontent\">\n\n<p><strong>Kutat\u00e1si ter\u00fcletek<\/strong><\/p>\n<p><strong>1) Az asztroglia architekt\u00far\u00e1ja.<\/strong><\/p>\n<p>Jellegzetes asztrogliaszerkezeteknek szerepelnek az agy egyedfejl\u0151d\u00e9s\u00e9ben, evol\u00faci\u00f3j\u00e1ban, \u00e9s a neuronok m\u0171k\u00f6d\u00e9si felt\u00e9teleinek biztos\u00edt\u00e1s\u00e1ban. Az asztrogliaszerkezeteket els\u0151sorban az intermedier filamentum-feh\u00e9rj\u00e9k (\u00e9retlen gli\u00e1ban nestin, vimentin, \u00e9rettben GFAP) kimutat\u00e1s\u00e1val vizsg\u00e1ljuk.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Az eddigi f\u0151bb eredm\u00e9nyek: <\/strong><\/p>\n<p>a GFAP eloszl\u00e1s\u00e1nak felt\u00e9rk\u00e9pz\u00e9se az egyes f\u0151bb gerinces csoportok reprezent\u00e1nsaiban, leg\u00e9rdekesebb vonatkoz\u00e1sa, hogy az idegrendszer evol\u00faci\u00f3ja, bonyolultabb\u00e1 v\u00e1l\u00e1sa ill. az asztrocit\u00e1k uralkod\u00f3v\u00e1 v\u00e1l\u00e1sa a GFAP visszaszorul\u00e1s\u00e1val, eloszl\u00e1sa egyenl\u0151tlens\u00e9g\u00e9nek fokoz\u00f3d\u00e1s\u00e1val ill. expresszi\u00f3ja fakultat\u00edvv\u00e1 v\u00e1l\u00e1s\u00e1val j\u00e1r.<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>Jelenlegi f\u0151 t\u00e9m\u00e1k:<\/strong><\/p>\n<p>-A GFAP helyi egyenl\u0151tlens\u00e9geinek kialakul\u00e1sa \u00e9s ennek jelent\u0151s\u00e9ge h\u00fcll\u0151kben, madarakban \u00e9s eml\u0151s\u00f6kben, \u00f6sszef\u00fcgg\u00e9se az extracellul\u00e1ris m\u00e1trixsszal ill. az agyi plaszticit\u00e1ssal.<\/p>\n<p>-A csontoshalak (amelynek a t\u00f6bbi gerinces\u00e9t\u0151l elt\u00e9r\u0151, \u2018everz\u00edv\u2019agya van ) gliaszerkezet\u00e9nek evol\u00faci\u00f3ja.<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>2) A glia \u00e9s a k\u00f6t\u0151sz\u00f6vet kapcsolata<\/strong><\/p>\n<p>Azokon a helyeken, ahol a k\u00f6zponti idegrendszer sz\u00f6vete k\u00f6t\u0151sz\u00f6vettel \u00e9rintkezik, els\u0151sorban az erek k\u00f6r\u00fcl \u00e9s az agyburkok ment\u00e9n, a k\u00f6t\u0151sz\u00f6vettel a glia l\u00e9tes\u00edt kapcsolatot. Ezeknek a kapcsolatoknak nagy jelent\u0151s\u00e9ge van az agy fejl\u0151d\u00e9s\u00e9ben, vaszkulariz\u00e1ci\u00f3j\u00e1ban, a v\u00e9r-agy g\u00e1tn\u00e1l, az agyi \u00e9relv\u00e1ltoz\u00e1sokban, az agysz\u00f6vet s\u00e9r\u00fcl\u00e9sek ut\u00e1ni v\u00e1laszaiban, a revaszkulariz\u00e1ci\u00f3ban. A k\u00f6zponti \u00e9s a perif\u00e9ri\u00e1s idegrendszer egyik alapvet\u0151 sz\u00f6vettani k\u00fcl\u00f6nbs\u00e9ge \u00e9ppen az, hogy gli\u00e1juknak a k\u00f6rnyez\u0151 k\u00f6t\u0151sz\u00f6vethez val\u00f3 viszonya elt\u00e9r\u0151, aminek szerepe lehet az elt\u00e9r\u0151 regener\u00e1ci\u00f3s k\u00e9pess\u00e9gben is. Az asztroglia a k\u00f6zponti idegrendszer k\u00f6r\u00fcl egy egys\u00e9ges z\u00e1r\u00f3r\u00e9teget (glia limitans externa) k\u00e9pez, amely folytat\u00f3dik a perivaszkul\u00e1ris gli\u00e1ban, \u00e9s amelyet egy lamina basalis v\u00e1laszt el a szervezet \u00e1ltal\u00e1nos k\u00f6t\u0151sz\u00f6vetes ter\u00e9vel. Ez\u00e9rt vizsg\u00e1ljuk a laminin eloszl\u00e1s\u00e1t, valamint a receptorai\u00e9t, els\u0151sorban a disztroglik\u00e1nt -disztrofin komplexet, az idegrendszer fejl\u0151d\u00e9se sor\u00e1n ill. s\u00e9r\u00fcl\u00e9seit k\u00f6vet\u0151en, a glia limitans helyre\u00e1ll\u00edt\u00e1sakor ill. az \u00e9rben\u00f6v\u00e9sek k\u00f6r\u00fcl.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Az eddigi f\u0151bb eredm\u00e9nyek<\/strong><\/p>\n<p>A komponensek detekt\u00e1lhat\u00f3s\u00e1ga expresszi\u00f3juk mellett funkcion\u00e1lis \u00e1llapotukt\u00f3l is f\u00fcgg.<\/p>\n<p>A v\u00e1ltoz\u00e1sok m\u00e1r a l\u00e9zi\u00f3 ut\u00e1ni els\u0151 percekben l\u00e9trej\u00f6nnek<\/p>\n<p>A disztroglik\u00e1n a csontoshalak fejlettebb form\u00e1iban nem mutathat\u00f3 ki, a t\u00f6bbi \u00e1llkapcsos gerincesen igen.<\/p>\n<p>Az extracellul\u00e1ris m\u00e1trix, a laminin \u00e9s disztroglik\u00e1n jellemz\u0151 eloszl\u00e1s\u00e1nak le\u00edr\u00e1sa a v\u00e9r-agy g\u00e1t n\u00e9lk\u00fcli cirkumventrikul\u00e1ris szervekben, ezek \u00f6sszefgg\u00e9se<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>Jelenlegi f\u0151 t\u00e9m\u00e1k:<\/strong><\/p>\n<p>A v\u00e9r-agy g\u00e1t-mentes cirkumventrikul\u00e1ris szervek elhat\u00e1rol\u00f3d\u00e1sa az agy\u00e1llom\u00e1ny m\u00e9lyebb r\u00e9szei fel\u00e9, az extracellul\u00e1ris m\u00e1trix lehets\u00e9ges szerepe.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>3) A reakt\u00edv glia vizsg\u00e1lata<\/strong><\/p>\n<p>Az asztroglia az agyi s\u00e9r\u00fcl\u00e9sekre jellegzetes reakci\u00f3val v\u00e1laszol, amelynek szerepe van a s\u00e9r\u00fcl\u00e9s k\u00f6vetkezm\u00e9nyeinek alakul\u00e1s\u00e1ban, a neuroprotekci\u00f3ban, a revaszkulariz\u00e1ci\u00f3ban, a glia limitans \u00e9s a v\u00e9r-agy g\u00e1t helyre\u00e1ll\u00edt\u00e1s\u00e1ban, ill. felel\u0151snek tartj\u00e1k a p\u00e1lyaregener\u00e1ci\u00f3 elmarad\u00e1s\u00e1\u00e9rt. Ez a szerepe a gli\u00e1nak az evol\u00faci\u00f3val, ill. az idegsz\u00f6vetnek az egyedfejl\u0151d\u00e9s sor\u00e1n val\u00f3 \u2018\u00e9r\u00e9s\u00e9vel\u2019 j\u00f6n l\u00e9tre.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Eddigi f\u0151bb eredm\u00e9nyek: <\/strong><\/p>\n<p>&#8211; A r\u00e1jaagyban az eml\u0151s\u00f6k\u00e9hez hasonl\u00f3an az asztrocit\u00e1k domin\u00e1lnak, de az eml\u0151sagy\u00e9hoz hasonl\u00f3 gliareakci\u00f3 nem alakul ki..<\/p>\n<p>&#8211; Mad\u00e1ragyban tal\u00e1ltunk gliareakci\u00f3t, de h\u00fcll\u0151agyban nem, teh\u00e1t a reakci\u00f3 k\u00e9pess\u00e9ge a mad\u00e1r- \u00e9s az eml\u0151sagyban egym\u00e1st\u00f3l f\u00fcggetlen\u00fcl jelent meg.<\/p>\n<p>&#8211; Fejl\u0151d\u0151 patk\u00e1nyagyban siker\u00fclt tal\u00e1lni olyan agys\u00e9r\u00fcl\u00e9si modellt, ahol a gliareakci\u00f3 nem g\u00e1tolja, s\u0151t, seg\u00edti az axonn\u00f6veked\u00e9st.<\/p>\n<p>&nbsp;<\/p>\n\n<\/div><h2 class=\"tabtitle\">ENGLISH<\/h2>\n<div class=\"tabcontent\">\n\n<p><strong>Research fields<\/strong><\/p>\n<ul>\n<li><strong>The architecture of astroglia<\/strong><\/li>\n<\/ul>\n<p>The astroglia has characteristic architectures adapted to requests of the neural structures during both brain development and evolution. Their investigation occurs mainly the immunohistochemical detection of the glial intermedier filaments, GFAP jn the mature glia and vimentin and nestin in the immature glia<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Former main results<\/strong><\/p>\n<p>Mapping of GFAP in the representatives of the main vertebrate groups. The results suggest that during evolution and with the predominancy of astrtocytes accompanies with the territorial extension of withdraw of GFAP and its expression becomes facultative<\/p>\n<p>.<\/p>\n<p><strong>Present main topics:<\/strong><\/p>\n<p>The local differences and its evolutional and functional importance in the different groups of\u00a0 reptiles, birds and\u00a0 mammals; their possible correlation with the extracellular matrix and the local plasticity.<\/p>\n<p>The evolution of the astroglia of ray-finned fishes (Actinopterygii) which have a characteristic \u2019eversive\u2019 brain.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 2) The connection of astroglia with connective tissues<\/strong><\/p>\n<p>Where the CNS tissue contacts with connective tissue, mainly along vessels and cerebral envelopes, the astroglia contacts with it. This contact has great importance in brain development, vascularisation, the formation of the blood-brain barier (BBB), and the separation of CNS following damages. One of the principal differences between CNS and PNS is the different contact with the connective tissue, which may have principal role their different regenerative capability. The astroglial borderline (glia limitans) around the CNS continues in the perivascular glia and forms a basal lamina anchored by the dystrophin-dystroglycan complex (DGC).<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Former main results<\/strong><\/p>\n<p>-The immunohistologic detectability of DGC components changes characteristically following lesions. It takes place in the first minutes<\/p>\n<p>-The detectability may indicate their functional stage.<\/p>\n<p>-In bony fishes and and anurans dystroglycan is not detectable, probably due to is characteristic molecular binding (masking).<\/p>\n<p>&#8211; Description of laminin, dystroglycan and extracellular matrix components in the circumventricular organs lacking BBB related to their glial structure.<\/p>\n<p><strong>:<\/strong><\/p>\n<p><strong>3) Investigation of reactive glia<\/strong><\/p>\n<p>The CNS lesions evoke a characteristic glial reaction, which has essential role in the outcome of damage, neural protection, revascularisation, restoring of isolation and BBB, and failure of regeneration. This role is a result of evolution and brain development.\u00a0<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Former main results<\/strong><\/p>\n<ul>\n<li>The above described glial reaction is confined to mammals and birds.<\/li>\n<li>Since the glial reactionis not found even in reptiles, its capability developed indepently in mammals and birds, where astrocytes are the predominant astroglial structures.<\/li>\n<li>Even in the brains of rays and skates, where astrocytes are also predominant, no reactive gliosis was found. It indicates that astrocyte predominance is a condition \u2019sine qua non\u2019 but insufficient alone.<\/li>\n<li>There is a lesion model in immature rat brain, in which the astroglial reaction does not inhibit but possibly promote axon growth.<\/li>\n<\/ul>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Vezet\u0151 \/ HEAD OF THE LAB: Dr. K\u00e1lm\u00e1n Mih\u00e1ly\u00a0 &#8211; egyetemi tan\u00e1r, az MTA doktora \/\u00a0Ph.D., D.Sc., full professor Munkat\u00e1rsak \/ STAFF: Seb\u0151k Oliv\u00e9r Marcell &#8211; tudom\u00e1nyos seg\u00e9dmunkat\u00e1rs \/ assistant research fellow \u0150z Andrea -asszisztens \/ technician Kutat\u00e1si ter\u00fcletek 1) Az asztroglia architekt\u00far\u00e1ja. Jellegzetes asztrogliaszerkezeteknek szerepelnek az agy egyedfejl\u0151d\u00e9s\u00e9ben, evol\u00faci\u00f3j\u00e1ban, \u00e9s a neuronok m\u0171k\u00f6d\u00e9si felt\u00e9teleinek &hellip;<\/p>\n","protected":false},"author":12,"featured_media":0,"parent":471,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[],"class_list":["post-1265","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/semmelweis.hu\/anatomia\/wp-json\/wp\/v2\/pages\/1265","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/semmelweis.hu\/anatomia\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/semmelweis.hu\/anatomia\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/anatomia\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/anatomia\/wp-json\/wp\/v2\/comments?post=1265"}],"version-history":[{"count":6,"href":"https:\/\/semmelweis.hu\/anatomia\/wp-json\/wp\/v2\/pages\/1265\/revisions"}],"predecessor-version":[{"id":19859,"href":"https:\/\/semmelweis.hu\/anatomia\/wp-json\/wp\/v2\/pages\/1265\/revisions\/19859"}],"up":[{"embeddable":true,"href":"https:\/\/semmelweis.hu\/anatomia\/wp-json\/wp\/v2\/pages\/471"}],"wp:attachment":[{"href":"https:\/\/semmelweis.hu\/anatomia\/wp-json\/wp\/v2\/media?parent=1265"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/semmelweis.hu\/anatomia\/wp-json\/wp\/v2\/categories?post=1265"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/semmelweis.hu\/anatomia\/wp-json\/wp\/v2\/tags?post=1265"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}