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Translational neuropharmacology research group

Members:

Projects
Centrally induced gastroprotective action of substance P (SP)
Glycine transporter type 1 (GlyT-1) – potential therapeutic target in retinal neurodegenerative disorders
Imidazoline receptors are not involved in the regulation of gastric motility
Hearing and deafness: molecular mechanisms and therapeutic approaches
Looking for new ways for analgesia

Experimental Methods
•   Measurement of the electrophysiological parameters of receptor pool and receptor kinetics of in vitro isolated organs.
•   Immunohistochemistry
•   In vivo antinociceptive tests, tolerance-dependence measurments, intracerebral, spinal application methods
•   Electrochemical HPLC measurement of transmitter release in isolated organs (rabbit ear artery, rabbit pulmonary artery, rat vas deferens, etc) and to detect specific enhancer effect in isolated brain regions (tuberculum olfactorium, substantia nigra, striatum, raphe, locus coeruleus).
•   Measuring transmitter release from in vitro tissue preparations (GABA, endomorphine 2, CGRP, [3H]-norepinephrine, dopamine, serotonin) by radioactive isotope and immunological technics
•   Measuring enhancer activity on cell cultures (PC12, HBEC) and stroke models
•    In vivo ulcer models
•    Measurement of gastrointestinal motility in vivo and in vitro
•    Acute and chronic inflammatory models
•    Measurement of microcirculation with Laser Doppler flowmetry
•    Stereotaxic injection into given brain nuclei
•    Genotyping of KO-mice with PCR
•    Patch clamp method
•    Brain microdialysis in anesthetised and conscious rats. Measurements of biogenic amines and amino acids with HPLC and electrochemistry.
•    Measurements of radiolabelled neurotransmitter release from isolated brain slices and retina preparation using superfusion techniques.
•   Functional imaging (Ca2+, ROS, mitochondrial membrane potential)
•   Objective audiometry on mice and rats (ABR)
•   Hearing loss models (aminoglycoside- and noise-induced hearing loss)

 Main cooperators
•     Department of Organic Chemistry, Semmelweis University
•     Department of Pharmacodynamics, Semmelweis University
•     Department of Pharmacology and Pharmacotherapy, University of Debrecen
•     Albert-Ludwigs University of Freiburg, Ins. of Experimental and Clinical Pharmacology and Toxicology , Freiburg, Germany
•     Karl-Franzens University of Graz, Dept. of Experimental and Clinical Pharmacology, Graz, Austria
•     Medical Faculty of Parma, Dept. of Pharmacology, Parma, Italy
•     University of Leipzig, Rudolph-Boehm Institute of Pharmacology and Toxicology, Leipzig, Germany
•     University of Coimbra, Center for Neuroscience, Coimbra, Portugal