Font Sizes: A A A

QMB (QUANTITATIVE AND MEDICAL BIOCHEMISTRY)

LECTURES

  • Time: Friday 10.15-13.00
  • Location: Room 239, PPKE ITBK 1083 Budapest, Práter u. 50/A
Feb_11 10.15-13.00 Introduction to metabolism. Metabolic pathways, kinetic and thermodynamic approaches to their structure. Metabolome, general principles in metabolic control. Enzyme-catalyzed reactions. Enzyme activity assays. Mechanism of enzyme action, structural basis of catalytic effects. Coenzymes, cofactors. Factors affecting the catalytic efficiency of enzymes. (KK)
Feb_18 10.15-13.00 Structural aspects of the control of enzyme activity. Allosteric and active site directed effects. Proteolytic activation and inactivation. Reversible covalent modifications. Methods for evaluation of enzyme action and its modulation. Spectrophotometric and fluorimetrc procedures. Zymography. Surface pasmon resonance. Microcalorimetry. (KK)
Feb_25 10.15-13.00 Enzyme kinetic models. Briggs-Haldane steady-state approach and non-steady state systems. Kinetic parameters and their interpretation in the context of metabolic pathways. In vivo significance of kinetic parameters. (KK)
Mar_01 10.15-13.00 Metabolic control analysis. Control, elasticity and response coefficients in metabolic pathways. Practical applications in drug research. (KK)
Mar_08 10.15-13.00 Stochastic nature of experimentally determined parameters in biochemistry. Statistical error and probability distribution of estimates. Models and simulations in enzyme kinetics. (KK)
Mar_22 10.15-13.00 Interfacial enzymology of blood coagulation. (KK)
Mar_29 10.15-13.00 Fuel utilization in the brain. Metabolism of glia cells and neurons. Molecular basis of neurotransmission. Structure of the synapsis. Synthesis, storage and release of neurotransmitters. (LT)
Apr_05 10.15-13.00 Glutamatergic neurotransmission. Glutamate-related excitotoxicity. Cholinergic neurotransmission. Dopaminergic and serotoninergic neurotransmission with pathological aspects. (LT)
Apr_12 10.15-13.00 Adrenergic neurotransmission in the cardiovascular system. Intracellular Ca2+ signalling
May_03 10.15-13.00 General principles of hormone action. Signal transduction of insulin. Effects of insulin on the cellular metabolome. Dysregulation of the metabolic networks in diabetes. (BT)
May_10 10.15-13.00 The metabolic roles of liver. Dysregulation of liver metabolism in disease. Acute liver failure and chronic liver diseases. The metabolism of alcohol. (BT)
May 17 10.15-13.00 Metabolism of the well-fed state and fasting. Obesity. Atherosclerosis. (BT)

Seminars/practical lessons

  • Location: Semmelweis University EOK, 1094 Budapest, Tűzoltó u. 37-47, Biochemistry lab #3.
  • Time:

April 5, 13.50-16.20 (Prof Kolev) Computer simulated enzyme kinetics 1. Continuous and end-point enzyme assays. Adjusting the conditions of the simulated experiment in accordance with the assumptions of the steady-state approach. Best experimental guess of the model parameters and their confidence intervals.

April 12, 13.50-16.20 (Prof Kolev) Computer simulated enzyme kinetics 2. Application of enzyme kinetics for solution of practical problems. Identification of physiological substrates and high control-coefficient steps in metabolic pathways. Identification of amino acids with critical function in the catalytic mechanism of enzymes.
May 3, 13.50-16.20 (Dr Törőcsik) Basics of carbohydrate metabolism. Glucose metabolism, glycolysis.  Gluconeogenesis in liver. Citric acid cycle. Terminal oxidation.
May 10, 13.50-16.20 (Dr Törőcsik) Lipid metabolism. Synthesis and oxidation of fatty acids. Metabolism of amino acids and ammonia.
May 17, 13.50-16.20 (Dr Törőcsik) Biochemical methods in medical practice: measurement of blood glucose, cholesterol and triglyceride. Evaluation of mitochondrial oxidation. Isoenzyme (pyruvate kinase) and transaminase activity measurement.
 
 

DOWNLOAD

Recommended literature

  • Orvosi Biokémia – Egyetemi tankönyv, Szer­kesztette: Ádám Veronika, Semmelweis Kiadó, Budapest 2016
  • Athel Cornish-Bowden: Fundamentals of enzyme kinetics. Wiley-Blackwell, Weinheim, 2012
  • Thomas M. Devlin: Textbook of biochemistry with clinical correlations. Wiley-Liss, New York, 2012
  • David Fell: Understanding the control of metabolism. Portland Press, London, 2003

QMB - Quantitative and medical biochemistry


The Biochemistry course offers a concise overview of the dynamic metabolic networks in the cell focusing on the general principles of enzyme kinetics, structure and control of metabolic pathways. Aspects essential for future specialists in Biotechnology are emphasized: modern biochemical techniques in the characterization of intermolecular interactions and enzyme action, in silico modelling of biochemical processes and systems. Medical orientation is implemented with discussion of the molecular basis of selected diseases with major public health impact (cardiovascular, neurodegenerative diseases) focusing on the molecular targets of therapy. Students participate in formal lectures, tutorials and computer-simulated practical lessons.