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QMB – 2019/2020

Lectures - timetable

Dear students,

Next week we resume our course according to the schedule below. All lectures and seminars will be delivered through the Zoom webconferencing system that allows your interactive participation. What you need for your participation is a reliable internet connection and a computer with a web camera and a microphone. The day before each class you will receive an invitation e-mail with the link to join the web lecture or seminar. All classes will be recorded and the video recordings will be available for online access till the end of the examination session. All presentation slides will also be available on our website as usual. The course requirements will not be changed: you have to submit the written course work on the quantitative part and take the oral exam on the medical part (if necessary the oral exam will be held also in the Zoom system).

Regards,
Prof Krasimir Kolev

Digital remote lectures on the Zoom platform starting March 27.

March 27, 10.15-13.00 (Prof Kolev)

Interfacial enzymology of blood coagulation.

April 3, 10.15-13.00 (Prof Tretter)

Fuel utilization in the brain. Metabolism of glia cells and neurons.

Molecular basis of neurotransmission. Structure of the synapsis. Synthesis, storage and release of neurotransmitters.

April 17, 10.15-13.00 (Prof Tretter)

Glutamatergic neurotransmission. Glutamate-related excitotoxicity.

Cholinergic neurotransmission. Dopaminergic and serotoninergic neurotransmission with pathological aspects.

April 17, 13.50-16.20 (Prof Tretter)

Adrenergic neurotransmission in the cardiovascular system. Intracellular Ca2+ signalling

April 24, 10.15-13.00 (Dr Törőcsik)

General principles of hormone action. Signal transduction of insulin.

Effects of insulin on the cellular metabolome. Dysregulation of the metabolic networks in diabetes.

May 8, 10.15-13.00 (Dr Törőcsik)

The metabolic roles of liver. Dysregulation of liver metabolism in disease. Acute liver failure and chronic liver diseases. The metabolism of alcohol.

May 15, 10.15-13.00 (Dr Törőcsik)

Metabolism of the well-fed state and fasting. Obesity. Atherosclerosis.

Seminars/practices - timetable

Dear students,

Next week we resume our course according to the schedule below. All lectures and seminars will be delivered through the Zoom webconferencing system that allows your interactive participation. What you need for your participation is a reliable internet connection and a computer with a web camera and a microphone. The day before each class you will receive an invitation e-mail with the link to join the web lecture or seminar. All classes will be recorded and the video recordings will be available for online access till the end of the examination session. All presentation slides will also be available on our website as usual. The course requirements will not be changed: you have to submit the written course work on the quantitative part and take the oral exam on the medical part (if necessary the oral exam will be held also in the Zoom system).

Regards,
Prof Krasimir Kolev

Location: Zoom webconferencing system.

March 27, 13.50-16.20 (Prof Kolev)

Computer simulated enzyme kinetics 1. Continuous and end-point enzyme assays. Adjusting the conditions of the simulated experiment in accordance with the assumptions of the steady-state approach. Best experimental guess of the model parameters and their confidence intervals.

April 3, 13.50-16.20 (Prof Kolev)

Computer simulated enzyme kinetics 2. Application of enzyme kinetics for solution of practical problems. Identification of physiological substrates and high control-coefficient steps in metabolic pathways. Identification of amino acids with critical function in the catalytic mechanism of enzymes.

April 24, 13.50-16.20 (Dr Törőcsik)

Basics of carbohydrate metabolism. Glucose metabolism, glycolysis.  Gluconeogenesis in liver. Citric acid cycle. Terminal oxidation.

May 8, 13.50-16.20 (Dr Törőcsik)

Lipid metabolism. Synthesis and oxidation of fatty acids. Metabolism of amino acids and ammonia.

May 15, 13.50-16.20 (Dr Törőcsik)

Biochemical methods in medical practice: measurement of blood glucose, cholesterol and triglyceride. Evaluation of mitochondrial oxidation. Isoenzyme (pyruvate kinase) and transaminase activity measurement.

Downloads

Recommended literature

Orvosi Biokémia – Egyetemi tankönyv, Szerkesztette: Ádám Veronika, Semmelweis Kiadó, Budapest 2016

Athel Cornish-Bowden: Fundamentals of enzyme kinetics. Wiley-Blackwell, Weinheim, 2012

Thomas M. Devlin: Textbook of biochemistry with clinical correlations. Wiley-Liss, New York, 2012

David Fell: Understanding the control of metabolism. Portland Press, London, 2003

Summary

Quantitative and medical biochemistry
36 h lectures and 15 h seminar/practical classes
Tutor: Prof Krasimir Kolev, MD, PhD, DSc

The Biochemistry course offers a concise overview of the dynamic metabolic networks in the cell focusing on the general principles of enzyme kinetics, structure and control of metabolic pathways. Aspects essential for future specialists in Biotechnology are emphasized: modern biochemical techniques in the characterization of intermolecular interactions and enzyme action, in silico modelling of biochemical processes and systems. Medical orientation is implemented with discussion of the molecular basis of selected diseases with major public health impact (cardiovascular, neurodegenerative diseases) focusing on the molecular targets of therapy. Students participate in formal lectures, tutorials and computer-simulated practical lessons.