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MED BIOCHEM 1 2015/2016

Lectures

Day Time Location
Wednesdays 11:10-12:20 Szent-Györgyi Albert lecture hall
Thursdays 12:35-13:45 Szent-Györgyi Albert lecture hall

Semmelweis University, EOK B uilding Budapest IX., Tűzoltó utca 37-47 H-1094

Topic # of lectures Lecturer  
Amino acids, proteins, enzymes 8 Prof. Sasvári, Mária MS
Bioenergetics-I 3 Dr. Chinopoulos, Christos CC
Subcellular biochemistry 3 Dr. Csala, Miklós MCs
Molecular biology 6 Dr. Bauer, Pál PB
  5 Dr. Törőcsik, Beáta TB
  3 Prof. Tretter, László LT

Schedule of lectures

Week     Topic  
01 03 Feb Amino acids. Amino acids as electrolytes. Structure and chirality of amino acids. Reactions of amino acids (MS)  
01 04 Feb Proteins. The peptide bond. Structure levels in proteins. Primary structure of proteins (MS)  
02 10 Feb Steric structure of globular proteins. Conformation of proteins. Purification of
proteins (MS)
 
02 11 Feb Structural characteristics of fibrous proteins. Collagen (MS)  
03 17 Feb Myoglobin and hemoglobin (MS)  
03 18 Feb Enzymes. Enzymes as biocatalysts, enzyme activity. Isoenzymes. Coenzymes (MS)  
04 24 Feb Enzyme kinetics: the Michaelis-Menten model. Mechanism of action of some important enzymes (serine proteases) (MS)  
04 25 Feb Reversible and irreversible inhibition of enzymes; competitive, non-competitive and uncompetitive inhibitors. Regulation of enzyme activity. Allosteric enzymes (MS)  
05 02 Mar Principles of bioenergetics: energy transformation, group transfer potential,  coupled reactions, substrate level phosphorylation (CC)  
05 03 Mar  Role of mitochondria in biological oxidation. Terminal oxidation (CC)  
06 09 Mar  Oxidative phosphorylation (CC)  
06 10 Mar  Compartmentation in eukaryotic cells. Membrane structure. Intracellular  membranes and organelles. Structure and function of the nucleus (MCs)  
07 16 Mar Movement of cellular organelles. Cytoskeleton, microfilaments, microtubuli,actomyosin. Mechanism of vesicular transport (MCs)  
07 17 Mar Metabolism and transport, the principle of metabolom. Metabolic profile of various organelles (endoplasmic reticulum, peroxisomes, lysosomes, mitochondria) (MCs)  
      Springbreak, Easter Monday (21-28 III)  
08 30 Mar Nucleic acids – structure and function. Bases, nucleosides, nucleotides, DNA structure, DNA denaturation, hybridization (PB)  
08 31 Mar DNA replication. Replication in prokaryotes, leading and lagging strand,
Okazaki fragments. DNA-dependent DNA polymerases. DNA ligase. Telomerase and topoizomerases. Replication in eukaryotes. Structure of  eukaryotic  chromosomes. Mitochondrial DNA. Nucleosome structure  (PB)
 
09 06 Apr DNA repair. Types of  DNA damage; mutations, frameshift, nonsense
mutations, mismatch repair. Coordination of repair and replication (TB)
 
09 07 Apr Transcription in prokaryotes. Structure of RNA; t-RNA, r-RNA, m-RNA, differences between prokaryotic and eukaryotic genomes.  Transcription complexes, initiation, elongation, termination in prokaryotes (PB)  
10 13 Apr Transcription in eukaryotes, RNA polymerases, promoters, enhancers,
silencers. Processing of mRNA, mechanism of splicing. Alternative splicing. mRNA editing (PB)
 
10 14 Apr The genetic code. Activation of tRNA. Mechanism of translation: initiation,
elongation, termination. Antibiotics. Posttranslational modifications of proteins (PB)
 
11 20 Apr Protein folding, sorting, quality control and transport into intracellular compartments. Ubiquitination and intracellular proteolysis (PB)  
11 21 Apr Regulation of gene expression in prokaryotes. The operon model; positive and negative regulation in the lac operon (LT)  
12 27 Apr Regulation of gene expression at the transcriptional level in eukaryotes. Role of chromatine structure; covalent and non-covalent chromatin modifying activities and DNA methylation (epigenetics) (LT)  
12 28 Apr  Post-transcriptional regulation of gene expression in eukaryotes. Regulation of mRNA stability; microRNAs. Translational control (LT)  
13 04 May Cell cycle in eukaryotes. Cyclins and cyclin dependent protein kinases. Proteases in the cell cycle. Regulation of G0/G1, G1/S and G2/M transitions. Integration  of DNA repair into the cell cycle (BT)  
13 05 May Principles of recombinant DNA technology: molecular cloning, restriction endonucleases. Genomic and cDNA libraries. Blotting techniques (Southern, Northern, Western) and their utilization. DNA microarrays (BT)  
14 11 May PCR, real-time PCR and their application in molecular biology. Recombinant vectors (reporter and expression vectors); synthesis of recombinant proteins. Transgenic, knock-out and knock-in animals in medical research. Human gene therapy (BT)  
 14 12 May The Human Genome Project and its results: organization and polymorphic nature of the human genome; implications for human traits and diseases. Genotyping assays (PCR-RFLP, PCR-ASA). Application of bioinformatics in biological and medical research  (BT)  

Lab. lessons

Week DD-DD MMM Laboratory lesson / seminar
01 01-05 Feb Carbohydrates (seminar)
02 08-12 Feb Precipitation and quantitative determination of proteins. Colour reaction of proteins (lab)
03 15-19 Feb Electrometric titration of amino acids (lab)
04 22-26 Feb Calculations: amino acids as buffers, protein purification and enzymology (consultation)  
05 29/05 Feb/Mar Experimental determination of the kinetic parameters of urease (lab)
06 07-11 Mar Midterm examination I (topics: amino acids, proteins, enzymes, bioenergetics)  
07 16-18 Mar   Lipids (seminar) 
  21-28 Mar Springbreak. Easter Monday
08 29/01 Mar/Apr Gel filtration (lab)
09 04-08  Apr SDS-PAGE gel electrophoresis and western blot* (lab)
10 11-15 Apr Paper and thin layer chromatography* (lab)
11 18-22 Apr Induction of ß-galactosidase (lab)
12 25-29 Apr Molecular Biology (seminar)
13 02-06 May Midterm examination II (topics: molecular biology)
14 09-13 May Laboratory exam (written, 15 min). RRestriction digestion of pGL3 basic vector and separation of DNA fragments electrophoresis(lab)

 

Schedule of laboratory practical lessons, consultations and seminars

Groups

Group Day Time Laboratory/seminar teacher
ED1 Wednesday 15.10-17.40 Nagy, Szilvia
ED2 Wednesday 08.00-10.30 Nagy, Szilvia
ED3 Wednesday 08.00-10.30 Végh, Miklós 
ED4 Wednesday 08.00-10.30 Vereczkei, Andrea 
       
EM01 Monday  11.4014.40  Sasvári, Mária
EM02 Wednesday  12.35-15.35  Bauer, Pál  
EM03 Monday  11.4014.40  Rónai, Zsolt 
EM04 Tuesday 15.30-18.30  Sipeki, Szabolcs  
EM05 Monday  15.00-18.00  Csanády, László
EM06 Wednesday  14.30-17.30  Spasokoukotskaya, Tatiana
EM07 Monday  11.4014.40  Bak, Judit 
EM08 Tuesday   15.30-18.30   Margittai, Éva 
EM09 Monday  11.4014.40  Csala, Miklós
EM10 Wednesday  14.30-17.30  Ambrus, Attila
EM11 Monday  11.4014.40  Sorum, Ben 
EM12 Tuesday   15.30-18.30   Dóczi, Judit 
EM13 Tuesday   14.00-17.00   Barta, Csaba
EM14 Monday  15.00-18.00 Sorum, Ben  
EM15 Monday  15.00-18.00  Stroe, Márta

Venue: Student laboratories of the Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, EOK Building, 1094 Budapest, Tűzoltó u. 37-47., 1st Floor, Passage „D”

Midterm 1

Midterm 2

Topic list

Requirements

Subject

The principal aim of the course is to provide an insight into the structure and function of biologically important molecules including amino acids, carbohydrates, lipids, vitamins, coenzymes (descriptive biochemistry), as well as proteins, enzymes and nucleic acids. This module is a prerequisite to the understanding of the intermediary metabolism of the cell. The cell biology unit describes the principles of organization of cells as well as the function of subcellular organelles. The molecular biology module aims at highlighting the storage and expression of genetic information throughout replication, transcription and translation as well as our current understanding of the regulation of gene expression, followed by an up-to-date summary of currently used methods in molecular biotechnology.

Exemption

  1. Exemption may be given only if students pass an exemption examination
  2. Prerequisite of participating in the exemption exam: previous studies and successful examinations in the subject Biochemistry (final exam) (Biochemistry I, II, III)
  3. Documents to be attached on the previous studies:

a transcript from the former University, containing the name of courses taken in Biochemistry, examination grades and a copy of your final BSc or MSc diploma (if you have)

a topic list of Biochemistry (bioenergetic, metabolism of carbohydrates, lipids, amino acids, nucleotides, biochemistry of tissues, neurobiochemistry, signal transduction, haemostasis and/or molecular biology (macromolecules) (for final exam) (Only official hardcopy version of documentation is accepted, we do not accept e-documentation).

the indication of the used textbook(s) is also recommended

will be organized by our examination committee.

  1. Oral examinations in Biochemistry
  1. The exemption examinations take place on the 5th or 6th week of the semester (Febr 29-04 or March 7-11, 2016); the deadline to present your documents is the end of the 2nd week (February 12, 2016).
  2. In case of unsuccessful exemption examination there are not any negative consequences. You have to attend laboratory lessons and seminars throughout the semester and sit for the regular examination at the end of the semester like the rest of students.

dr. Gergely Keszler

dr. István Léránt

teaching secretary of 1st year program in Chemistry and Biochemistry I.

teaching secretary of 2nd year program in Biochemistry II-III

keszler.gergely@med.semmelweis-univ.hu

lerant.istvan@med.semmelweis-univ.hu

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