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BIOCHEMISTRY II

 MEDICAL BIOCHEMISTRY, MOLECULAR AND CELL BIOLOGY II – (fall semester of 2016/2017 academic year)

Lectures

LECTURES – fall semester of 2016/2017 academic year

Location, time: Tuesday 16:50-18:00; Thursday 08:00-09:10 Szent-Györgyi Albert Lecture Hall, EOK-Builing, Semmelweis University Budapest IX., Tűzoltó utca 37-47 H-1094
Lecturers:

László Tretter MD, PhD, DSc (LT), József Mandl MD, PhD, DSc, Member of the Hungarian Academy of Sciences (JM), Mária Sasvári MD, PhD, DSc (MS),, Kraszimir Kolev MD, PhD, DSc (KK), Miklós Csala MD, PhD, DSc (MCs), Erzsébet Komorowicz MD, PhD Beáta Törőcsik MD, PhD (BT)

Week mm:dd Topic
01 09:06 Glycolysis. (MCs)
  09:08 Pyruvate dehydrogenase. (MCs)
02 09:13 Citrate cycle. (MCs)
  09:15 Gluconeogenesis. Glycogen metabolism. (JM)
03 09:20 Regulation of glycolysis and gluconeogenesis. (JM)
  09:22 Regulation of glycogen metabolism. (JM)
04 09:27 Regulation of blood glucose level. (JM)
  09:29 Diabetes mellitus. (JM)
05 10:04 Degradation and synthesis of various carbohydrates. (JM)
  10:06 Metabolism of lipids – overview. Absorption of lipids. (BT)
06 10:11 Oxidation of fatty acids, ketone bodies. (BT)
  10:13 Synthesis of fatty acids. (BT)
 07 10:18 Lipoprotein metabolism. (BT)
  10:20 Cholesterol homeostasis: sources of cholesterol in the cell, absorption of dietary cholesterol and inter-organ transport of cholesterol. Disposal of cholesterol and the role of bile acids. (KK)
  08 10:25 Regulatory mechanisms in cholesterol homeostasis, metabolic receptors and their function in the liver, intestine and blood vessel wall. Biochemistry of atherosclerosis. (KK)
  10:27 Biosynthesis of steroid hormones in the adrenal cortex. Effects of mineralocorticoids and glucocorticoids, regulation of their biosynthesis. Signal transduction of steroid hormone receptors, genomic  and non-genomic actions of steroid hormones. Congenital adrenal hyperplasias. (EK)
 09  11:03 Biosynthesis of sexual hormones. Cyclic changes in the ovaries, the cooperation of granulosa and theca interna cells. Placental progesterone and estradiol synthesis. (EK)
10 11:07 Eicosanoids: prostaglandins, thromboxanes and leukotrienes. The cyclooxygenase and the lipoxygenase pathways. Effects of eicosanoids on: platelet aggregation, gastric mucosal protection, myometrial contractility, inflammation. (EK) (Szent-Györgyi Albert lecture hall 17:40-18:50)
  11:08 Metabolism of amino acids: the fate of the amino group. (MS)
  11:10 Metabolism of amino acid: the fate of the carbon sceleton. (MS)
11 11:15 Biosynthesis of nonessential amino acids and other amino acid derivatives. (MS)
  11:17 Synthesis and catabolism of purine nucleotides. (MS)
12 11:22 Pyrimidine nucleotide metabolism. Synthesis of deoxyribonucleotides (MS)
  11:24 Metabolism of porphyrins and bile pigments (MS)
13 11:29 Metabolism of red blood cells. Red blood cell enzymopathies. (LT)
  12:01 Metabolism of skeletal and cardiac muscle, the role of SREBP, PPAR alpha and beta, (LT)
14 12:06 Intermediary metabolism of kidney, metabolism in kidney cortex and medulla. (LT)
  12:08 Regulation of the well-fed state and starvation. (LT)

Laboratory teachers

LABORATORY LESSONS  & SEMINARS – falll semester of 2016/2017 academic year

Laboratory lessons and seminars are held at laboratory rooms of the Deparment (EOK-Building, Budapest IX., Tűzoltó utca 37-47. H-1094, 1st floor, passage ‘C’)
Computer skills “Enzyme kinetics” (Week 4)) are held at laboratories of informatics of Semmelweis University.

    Groups of dentistry students    
Group Room# Day Time Laboratory/seminar teacher
ED1 #1 Wednesday 12:15-14:45 Ágota Vér  dr
ED2 #2 Wednesday 12:15-14:45  István Léránt dr
ED3 #3 Wednesday 12:15-14:45 Veronika Farkas dr
ED4 #4 Wednesday 12:15-14:45 Judit Bak dr / Ádám Nagy dr 
    Groups of medicine students    
EM01 #3 Tuesday 10:30-13:00  Christos Chinopoulos dr
EM02 #2 Friday 08:00-10:30 Nándor Müllner dr
EM03 #2 Friday 08:00-10:30 Erzsébet Tóth dr
EM04 #2 Monday 13:30-16:00 Beáta Törőcsik dr 
EM05 #4 Friday 08:00-10:30 Katalin Bartha dr 
EM06 #4 Monday 13:30-16:00 Miklós Csala dr 
EM07 #4 Tuesday 10:30-13:00 András Hrabák dr 
EM08 #4 Friday 08:00-10:30 Kraszimir Kolev prof 
EM09 #3 Monday 10:45-13:15 Erzsébet Komorowicz dr 
EM10 #1 Monday 10:45-13:15 Judit Bak dr / Tímea Komlódi dr 
EM11 #5 Monday 10:45-13:15 Kraszimir Kolev prof 
EM12 #1 Monday 10:45-13:15 Tamás Mészáros dr
EM13 #4 Monday 13:30-16:00 Zsófia Komáry dr 
EM14 #2 Tuesday 10:30-13:00 István Léránt dr / Beáta Törőcsik dr 
EM15 #3 Monday 13:30-16:00  Gergely Keszler dr

 

Schedule of laboratory lessons & seminars


Laboratory lessons and seminars are held at laboratory rooms of the Deparment (EOK-Building, Budapest IX., Tűzoltó utca 37-47. H-1094, 1st floor, passage ‘C’)
Computer skills “Enzyme kinetics” (Week  3 or week 3 and 4)) are held at laboratories of informatics of Semmelweis University.

 

Week Date  Laboratory lesson / seminar  
  (mm:dd)    
1. 09.05_09.09  
2. 09.12-09.16  Gene therapy (seminar)  
3. 09.19-09.23 New version: Competitive inhibition of succinate dehydrogenase  (laboratory lesson) Old version:     Competitive inhibition of succinate dehydrogenase
 
4. 09.26-09.30 Enzyme kinetics – computer simulation (date, time, location) (laboratory lesson)   
5. 10.03-10.07 Oxidative phosphorylation in mitochondria (laboratory lesson)  
6. 10.10-10-14 Lactic acidosis (seminar) for EM and ED groups
 
6. 10.15 on Sat EM4,6,9,10,11,12,13,15 Signaltransduction of insulin (consultation) on Saturday
 
7. 10.17-10.21 Allosteric regulation of pyruvate kinase (laboratory lesson)  
8. 10.24-10.28 Fructose intolerance & McArdle’s disease (seminar)  
9. 11.02-11.04 ED1,2,3,4 EM2,3,5,8: Digestion of lipids, role of bile acids (laboratory lesson)  
10. 11.09-11.11 ED1,2,3,4 EM2,3,5,8: Signaltransduction of insulin (consultation)  
10. 11.07-11.08 EM1,4,6,7,9,10,11,12,13,14,15: Digestion of lipids, role of bile acids (lab)  
11. 11.14-11.18 Determination of serum cholesterol and triglyceride.  (laboratory lesson)  
12. 11.21-11.25 Lipoprotein lipase, & carnitine deficiency (consultation)  
13. 11.28-12.02 Purification of trypsin by affinity chromatography & Substrate specificity of trypsin and chymotrypsin. (laboratory lesson)  
14. 12.05-12.09 Last week laboratory examination.
Hereditary hyperammonemias.& Vitamin B12 deficiency, methylmalonic acidemia (consultation)

Scientific articles for seminars (2015/2016) DOWNLOAD HERE (password)

Scientific articles for seminars (2014/2015) DOWNLOAD HERE (password)

Scientific articles for seminars (- 2013/2014) – DOWNLOAD HERE (user name  –  password)

Recommended books

retake-exam of midterm exams

The second oral retake of the 1st and 2nd midterm exams is held on Thursday  – Dec 1,  2016; 17:00 – (week 13); at the biochemistry  laboratory rooms  of Department of Medical Biochemistry; Budapest IX., Tűzoltó utca 37-47, 1st floor, passage ’C’), for those  students who haven’t passed either the 1st  or/and the 2nd  midterm exams. 

Details: Oral retake midterm exam

The examining committees will consist of 2 members and have the following composition:  (i) the Supervisor; (ii) and a Chair.

We ask you to come to our Student Affair Office (room 1.508) for 16:50 and contact there Mr Zsolt Ozsváth. The examining committee shall be chosen by lot.

The oral midterm exam (1st midterm examination) consists of 2 questions (bioenergetics and carbohydrates +seminar: gene therapy).

The oral midterm exam (2nd midterm examination) consists of 2 questions (metabolism of lipids + lipidtransport + seminars).

Details: Oral retake midterm exam

At the end of the examination, members of the examination committee, including the Chair, will vote.  If any modules of oral exam  show a status of ’1 (fail)’, – this means you have failed your oral retake of midterm examination.

Midterm exams

MIDTERM EXAMS (ME) – fall semester of 2016/2017 academic year

Students who failed the first retake exam may sit for  second retake held the 13th week of the semester. This second retake is an oral exam conducted by an examination committee. Students who failed the first retakes of both midterms 1 and 2 will be examined in the material of both midterms at the same time.

2nd – oral retake of midterm examinations 1 & 2:  Week 13. Dec 1 17:00 20:00 EOK Biochemistry laboratory rooms

Students who are ill at the time of the 1st midterms can compensate their absence on the Oct 20, 2016 (1st midterm exam – 1st retake). Students who are ill at the time of their 2nd mindterm exam can compensate their absence on the  Nov 17, 2016 at 17:00 18:20 at Szent-Györgyi Albert Lecture Hall, after presenting medical certificate.

The medical certificates are accepted by Mr. Zsolt Ozsváth, at our Students’ Affairs Office, Budapest IX., Tűzoltó utca 37-47, H-1094 1st floor, passage “C” room# 1.508 Phone: +36-1-4591500#60061, +36-20-6660061

Deadline for the presentation of the medical certificate: (i) 1st midterm examination: Oct 17, 2016 and (ii) 2nd midterm examination: Nov 14, 2016 (iii) oral exam: Nov 28.

Requirements

Neptun codes: AOKOBI290_2A; FOKOOBI159_2A

Credit: 6; 5

Lecturer: László Tretter MD, PhD, DSc, head of Department of Medical Biochemistry

Teaching secretary: István Léránt PhD, senior lecturer

Lectures: Tuesday 16:20-17:30 Thursday 09:20-10:30 (3 hours/week)

Szent-Györgyi Albert Lecture Hall, EOK-Building, Semmelweis University, Budapest IX., Tűzoltó utca 37-47. H-1094

Schedule of lectures

 

     
1 2015-09-08 Glycolysis. (MCs)
  2015-09-10 Formation of acetyl-CoA – pyruvate dehydrogenase. (MCs)
02 2015-09-15 Citrate cycle. (MCs)
  2015-09-17 Gluconeogenesis. Glycogen metabolism. (JM)
03 2015-09-22 Regulation of glycolysis and gluconeogenesis. (JM)
  2015-09-24 Regulation of glycogen metabolism. (JM)
04 2015-09-29 Regulation of blood glucose level. (JM)
  2015-10-01 Diabetes mellitus. (JM)
05 2015-10-06 Degradation and synthesis of various carbohydrates. (JM)
  2015-10-08 Metabolism of lipids – overview. Absorption of lipids. (LT)
06 2015-10-13 Oxidation of fatty acids, ketone bodies. (LT)
  2015-10-15 Synthesis of fatty acids. (LT)
07 2015-10-20 Lipoprotein metabolism. (LT)
08 2015-10-27 Cholesterol homeostasis: sources of cholesterol in the cell, absorption of dietary cholesterol and inter-organ transport of cholesterol. Disposal of cholesterol and the role of bile acids. (KK)
  2015-10-27 Regulatory mechanisms in cholesterol homeostasis, metabolic receptors and their function in the liver, intestine and blood vessel wall. Biochemistry of atherosclerosis. (KK) (17:40-18:50 Szent-Györgyi Albert lecture hall
  2015-10-29 Biosynthesis of steroid hormones in the adrenal cortex. Effects of mineralocorticoids and glucocorticoids, regulation of their biosynthesis. Signal transduction of steroid hormone receptors, genomic and non-genomic actions of steroid hormones. Congenital adrenal hyperplasias. (EK)
09 2015-11-03 Biosynthesis of sexual hormones. Cyclic changes in the ovaries, the cooperation of granulosa and theca interna cells. Placental progesterone and estradiol synthesis. (EK)
  2015-11-05 Eicosanoids: prostaglandins, thromboxanes and leukotrienes. The cyclooxygenase and the lipoxygenase pathways. Effects of eicosanoids on: platelet aggregation, gastric mucosal protection, myometrial contractility, inflammation. (EK)
10 2015-11-10 Metabolism of amino acids: the fate of the amino group. (MS)
  2015-11-12 Metabolism of amino acid: the fate of the carbon sceleton. (MS)
11 2015-11-17 Biosynthesis of nonessential amino acids and other amino acid derivatives. (MS)
  2015-11-19 Synthesis and catabolism of purine nucleotides. (MS)
12 2015-11-24 Pyrimidine nucleotide metabolism. Synthesis of deoxyribonucleotides. (MS)
  2015-11-26 Metabolism of porphyrins and bile pigments. (MS)
13 2015-12-01 Metabolism of red blood cells. Red blood cell enzymopathies. (LT)
  2015-12-03 Metabolism of skeletal and cardiac muscle, the role of SREBP, PPAR alpha and beta, (LT)
14 2015-12-08 Intermediary metabolism of kidney, metabolism in kidney cortex and medulla.(LT)
  2015-12-10 Regulation of the well-fed state and starvation. (LT)

Laboratory lessons and seminars

Laboratory lessons and seminars are held at laboratory rooms of Deparment of Medical Biochemistry. (EOK-Building, Budapest IX., Tűzoltó utca 37-47. H-1094, 1st floor, passage ‘C’).

Computer skills “Enzyme kinetics” (Week  3) are held at laboratories of informatics of Semmelweis University. For details – http://semmelweis.hu/biokemia/en/

Laboratory and seminar teachers

 

  Room# Groups of dentistry students    
Group Room# Day Time Laboratory/seminar teacher
ED1 #1 Thursday 10:45-13:15 Vér, Ágota dr
ED2 #2 Thursday 10:45-13:15 Léránt, István dr
ED3 #3 Thursday 10:45-13:15 Keszler, Gergely dr
ED4 #4 Thursday 10:45-13:15 Bak, Judit dr/Nagy, Ádám dr
    Groups of medicine students    
EM01 #3 Friday 08:00-10:30 Chinopoulos, Christos dr
EM02 #2 Friday 08:00-10:30 Müllner, Nándor dr
EM03 #2 Thursday 13:30-16:00 Mészáros, Tamás dr
EM04 #2 Friday 10:45-13:15 Törőcsik, Beáta dr
EM05 #4 Friday 08:00-10:30 Tóth, Erzsébet dr
EM06 #4 Friday 10:45-13:15 Csala, Miklós dr
EM07 #4 Monday 10:45-13:15 Hrabák, András dr
EM08 #4 Monday 08:00-10:30 Kolev, Kraszimir dr
EM09 #3 Friday 10:45-13:15 Komorowicz, Erzsébet dr
EM10 #1 Friday 08:00-10:30 Bak, Judit dr / Komlódi, Tímea dr
EM11 #5 Thursday 10:45-13:15 Kolev, Kraszimir prof
EM12 #1 Wednesday 08:30-11:00 Bartha, Katalin dr
EM13 #4 Wednesday 08:30-11:00 Komáry, Zsófia dr
EM14 #2 Wednesday 08:30-11:00 Léránt István dr / TörőcsikBeáta dr
EM15 #3 Wednesday 08:30-11:00 Varjú, Imre dr

Schedule of laboratory lessons and seminars

 

Week Date Laboratory lesson / seminar
  (mm-dd)  
1. 09.07_09.11 Safety rules for work in the laboratory & Amidolytic activity of trypsin (laboratory lesson)
2. 09.14-09.17 Competitive inhibition of succinate dehydrogenase (laboratory lesson)
3. 09.21-09.25 Enzyme kinetics – computer simulation (data, time, location) (laboratory lesson)
4. 09.28-10.02 Gene therapy (seminar)
5. 10.05-10.09 Oxidative phosphorylation in mitochondria (laboratory lesson)
6. 10.12-10-16 Lactic acidosis (seminar)
7. 10.19-10.21 Allosteric regulation of pyruvate kinase (laboratory lesson)
8. 10.26-10.30 Fructose intolerance & McArdle’s disease (seminar)
9. 11.02-11.06 Digestion of lipids, role of bile acids (laboratory lesson)
10. 11.09-11.13 Signaltransduction of insulin (consultation)
11. 11.16-11.20 Determination of serum cholesterol and triglyceride (laboratory lesson)
12. 11.23-11.27 Lipoprotein lipase , & carnitine deficiency (consultation)
13. 11.30-12.04 Purification of trypsin by affinity chromatography & Substrate specificity of trypsin and chymotrypsin. (laboratory lesson)
14. 12.07-12.11 Last week laboratory examination.
Hereditary hyperammonemias.& Vitamin B12 deficiency, methylmalonic acidemia (consultation)

Requirements for acknowledgement of the semester

  1. Participation in the laboratory practicals, consultations and seminars is obligatory; students have to sign the attendance sheets at the end of every lesson. In case of more than three absences from the practical lessons for any reason, the semester will not be acknowledged and the student is not allowed to sit for the semifinal exam. Missed practicals can be completed only in the same week at another group; certificate from the host teacher should be presented by the student to his/her own teacher. The schedule of practical lessons, consultations and seminars can be downloaded from the official homepage of the Institute.
  1. Both midterm examinations have to be passed before the commencement of the examination period

Midterm examinations

Two midterm examinations will be held in the weeks 6 and 10 of the semester, respectively (Oct. 13, 2016 17:30; Nov 10, 2016 17:30). For details visit our homepage: http://semmelweis.hu/biokemia/en/

25 multiple choice type questions and 2 biochemistry problem sets (panels) are asked. Students may obtain 1 score for each correctly solved multiple choice questions. The both problem sets are evaluated by your own laboratory teachers (points: 0, 2, 3, 4, 5). We calculate the sum of your scores and points of your midterm examination and the tests are graded by the proposed limits of the table below:

Proposed limits for the marks of midterm are:

Sum of the points and scores Grade of the midterm examination
0-18 1 (fail)
19-22 2 (pass)
23-26 3 (satisfactory)
27-30 4 (good)
31-35 5 (excellent)

The grade for the midterm exam is 1 (fail): (i) if the sum of the scores and the grades is 18, or less (ii) each of the problems are evaluated by 0. Passing both midterms is a prerequisite to acknowledgement of the semester.

Failed midterms might be retaken twice.

The first retakes should be performed on the 20th of October 2016 (retake of midterm I) and on 17th of Nov (retake of midterm II) (for details please, click the homepage of Department of Medical Biochemistry). Students having failed the first retake(s) might sit for the second retake (Dec 1, 2016; 17:30). The second retake is an oral exam conducted by an examination committee. Students having failed the first retakes of both midterms I and II will be examined in the material of both midterms at the same time.

Grading of the performance of the laboratory work

Laboratory work performance assessment is a part of the semi-final exam. The practical work and its documentation is assessed by the laboratory teachers. (0 or 1 point). Maximal number of the collected points for laboratory work in this semester is 8 and these points are equivalent with 3 ‘lab-bonus’ points for the semi-final examination.

 

7-8 points for laboratory work = 3 ‘lab-bonus’ points
5-6 points for laboratory work = 2 ‘lab-bonus’ points
4 points for laboratry work = 1 ‘lab-bonus’ points

Semi-final exemination

The grade of the semi-final examination is based on four elements:

  1. theoretical written test of biochemistry problem set and multiple choice type tests (T)
  2. laboratory examination (L)
  3. grades of midterm examinations (M)
  4. bonus points‘ collected in the course of the semester (B) (see also ‘grading of the performance of the laboratory work’)

Theoretical written test (T)

The biochemistry problem sets and multiple choice type test questions are based on the recommended textbook, the lectures and the seminars. The theoretical written test is divided in three blocks (i) block#A set of biochemistry problems (containing questions for 30 points) (ii) block#B 30 multiple choice type test questions (carbohydrates, metabolic integration) (iii) block#C 30 multiple choice type test questions (lipids, amino acids, nucleotides, porphyrins). Correct answer for a biochemistry problems may result in max 1-3 points. The correct solution for a multiple choice type question results in 1 point. Futher points are added to the scores of block#B and block#C on the basis of the midterm examinations (M) of the fall semester of the 2016/2017 academic year.1 Students may pass the semi-final examination with minimal scores of 15, 15, 15 for the block#A, block#B and block#C, respectively. 2 minutes is given for the solutions of each multiple choice type test questions and 30 minutes for the set of biochemistry problems.

Laboratory examination (L)

The laboratory examination is an obligatory part of the semi-final examination. Students get one question based on the attached „list of laboratory questions” and answer it in written form (in 15 Min). These tests are graded by 0, 1, 2 points. Students can not pass the semi-final examination with 0 point from the practical exam. Department of Medical Biochemistry gives the students a chance to write their laboratory test on the last week of the semester (14th week; last week laboratory exam). These tests are graded by 1 or 2 points; unsuccessful tests are only marked as ‘not accepted’.

Results of the midterm examinations (M)

Grades of the 1st midterm examinations as points are added to the scores of block#B, the grades of 2nd midterm examination as points are added to the block#C.

Bonus points collected in the course of the semester (B)

Maximal number of ‘bonus points’ for the fall semester is 7. These bonus points can be collected in the course of fall semester according to the following scheme:

from the grading of the experimental work (‘lab-bonus’ points) (see ‘grading of the performance of the laboratory work’)

  • 3 ‘lab-bonus’ points, if the sum of the collected experimental points is 7 or 8
  • 2 ‘lab-bonus’ points, if the sum of the collected experimental points is 5 or 6
  • 1 point, if the sum of the collected experimental points is at least 4

from oral presentations on the seminars (‘presentation-bonus’ point) accompanied by powerpoint presentations or written summaries in electronic format 4 points, (if the student understands the content of the presented paper, emphasizes the biochemical interrelations and interprets the presented article at the expected level of knowledge)

Grades of the semi-final examination

Total scores from the 4 elements of the semi-final examination = T + L + M + B

T + L + M + B Grade
if any of block#A, block#B or block#C contains 14 scores or less; if the laboratory examination is graded by 0; if the sum of T+L+M+B 55 or less Fail (1)
56-69 Pass (2)
70-82 Satisfactory (3)
83-95 Good (4)
96-109 Excellent (5)

Students who pass block#A#B#C and the sum of T+L+M+B is 55 or more, but fail on the practical essay have to retake only the practical essay when they repeat the semifinal exam; students who pass the laboratory examination but fail on the written test, have to retake the blocks#A#B#C. Those who want better grade are entitled to rewrite also the block#A#B#C, however, with the risk of performing worse.

‘1 point rule’ if any of the blocks contains 14 point and the other two blocks contain 15 points or more (T) + the laboratory exam is evaluated by either 1 or 2 points (L), the grade for final-examination is 2 (pass) if the sum of scores and points (T+L+M+B) are 59 points or higher.

Biochemistry Competition

Results of the biochemistry competition (fall semester of 2016/2017 academic year)

Date: Dec 7, 2016, 17:30 EOK-Building, Szent-Györgyi Albert Lecture Hall

Material: lectures, seminars and laboratory lessons of the fall semesters of 2016/2017
Students reaching at least 75 % will be exempted from the examination by mark 5. Other exemptions are not possible
Conditition: minimum grades 3 at both midterms and registration for /Medical/ Biochemistry, Molecular and Cell Biology I in the spring semester of 2015/2016 academic year, and registration for /Medical/ Biochemistry, Molecular and Cell Biology II in the fall semester of 2016/2017 academic year.

Registration starts on the 1 Dec, 2016.

Semi-final examinations

Registration for /Medical/ Biochemistry, Molecalar and Cell Biology II and modification of the examination dates: electronically, via the Neptun-portal, http://net.neptun.sote.hu

Recommended textbooks, manuscripts, handouts:

1Results of midterm examinations, laboratory exams of earlier studies are not accepted.

Laboratory exam (Last week labexam)

A facultative laboratory exam is held for our regular students on the 14th week of the fall semester of 2016/2017 academic year. The test is held in the first 15 Min of the last week seminar, postponement of the exam is not allowed.

Date: 05-09 Dec, 2016, Laboratory rooms of the Department

Material: the topics of the laboratory lessons of the fall semester – 2016/2017 academic year

Evaluation: the tests are evaluated by 2 or 1 point/s or they are not accepted (NA).

Results are published until Dec 12, 2016

If you pass our ‘L‘ test (laboratory exam), your semi-final exam consists ‘only’ the ‘T’ test  (theoretical written test). If your ‘L’ test is not accepted (na), you have to pass the both parts – ‘L’ and ‘T’ test in December or in January.

Result of the tests is published on the 12th  of Dec (link ‘lecture slides’)

  • Experimental determination of enzyme kinetic parameters (Computer skills)
  • Competitive inhibition of succinate dehydrogenase by malonate
  • Mitochondrial oxidation I.: Experimental conditions for determination of oxygen consumption by intact mitochondria. Definition and experimental measurement of P/O ratio.
  • Mitochondrial oxidation II.: Please draw the curve of mitochondrial oxygen consumption after consecutive addition of glutamate/malate – ADP – atractylozide – DNP – oligomycin – CN-. Explain the curves briefly.
  • Differential regulatory mechanisms of behaviour of L- and M-type isoenzymes of pyruvate kinase: effect of allosteric ligands on activity
  • Digestion of lipids, determination of lipase activity
  • Plasma lipoproteins and lipid determination of plasma cholesterol and triglyceride level
  • Isolation of trypsin by affinity chromatography. Characterization of the substrate specificity of trypsin and chymotrypsin.

Competition

BIOCHEMISTRY COMPETITION – fall semester of 2016/2017 academic year

Results of the biochemistry competition (fall semester of 2016/2017 academic year)

Date: Dec 7, 2016, 17:30 EOK-Building, Szent-Györgyi Albert Lecture Hall

Material: lectures, seminars and laboratory lessons of the fall semesters of 2016/2017
Students reaching at least 75 % will be exempted from the examination by mark 5. Other exemptions are not possible

Conditition: minimum grades 3 at both midterms and registration for /Medical/ Biochemistry, Molecular and Cell Biology I in the spring semester of 2015/2016 academic year, and registration for /Medical/ Biochemistry, Molecular and Cell Biology II in the fall semester of 2016/2017 academic year.

Registration starts on the 1 Dec, 2016.

Semi-final examination

Semi-final exemination

Contact your tutor! Registration for office hours

The grade of the semi-final examination is based on four elements:

  1. theoretical written test of biochemistry problem set and multiple choice type tests (T)
  2. laboratory examination (L)
  3. grades of midterm examinations (M)
  4. bonus points‘ collected in the course of the semester (B) (see also ‘grading of the performance of the laboratory work’)

Theoretical written test (T)

The biochemistry problem sets and multiple choice type test questions are based on the recommended textbook, the lectures and the seminars. The theoretical written test is divided in three blocks (i) block#A set of biochemistry problems (containing questions for 30 points) (ii) block#B 30 multiple choice type test questions (carbohydrates, metabolic integration) (iii) block#C 30 multiple choice type test questions (lipids, amino acids, nucleotides, porphyrins). Correct answer for a biochemistry problems may result in max 1-3 points. The correct solution for a multiple choice type question results in 1 point. Futher points are added to the scores of block#B and block#C on the basis of the midterm examinations (M) of the fall semester of the 2016/2017 academic year.1 Students may pass the semi-final examination with minimal scores of 15, 15, 15 for the block#A, block#B and block#C, respectively. 2 minutes is given for the solutions of each multiple choice type test questions and 30 minutes for the set of biochemistry problems.

Laboratory examination (L)

The laboratory examination is an obligatory part of the semi-final examination. Students get one question based on the attached „list of laboratory questions” and answer it in written form (in 15 Min). These tests are graded by 0, 1, 2 points. Students can not pass the semi-final examination with 0 point from the practical exam. Department of Medical Biochemistry gives the students a chance to write their laboratory test on the last week of the semester (14th week; last week laboratory exam). These tests are graded by 1 or 2 points; unsuccessful tests are only marked as ‘not accepted’.

Results of the midterm examinations (M)

Grades of the 1st midterm examinations as points are added to the scores of block#B, the grades of 2nd midterm examination as points are added to the block#C.

Bonus points collected in the course of the semester (B)

Maximal number of ‘bonus points’ for the fall semester is 7. These bonus points can be collected in the course of fall semester according to the following scheme:

from the grading of the experimental work (‘lab-bonus’ points) (see ‘grading of the performance of the laboratory work’)

  • 3 ‘lab-bonus’ points, if the sum of the collected experimental points is 7 or 8
  • 2 ‘lab-bonus’ points, if the sum of the collected experimental points is 5 or 6
  • 1 point, if the sum of the collected experimental points is at least 4

from oral presentations on the seminars (‘presentation-bonus’ point) accompanied by powerpoint presentations or written summaries in electronic format 4 points, (if the student understands the content of the presented paper, emphasizes the biochemical interrelations and interprets the presented article at the expected level of knowledge)

Grades of the semi-final examination

Total scores from the 4 elements of the semi-final examination = T + L + M + B

T + L + M + B Grade
if any of block#A, block#B or block#C contains 14 scores or less; if the laboratory examination is graded by 0; if the sum of T+L+M+B 55 or less Fail (1)
56-69 Pass (2)
70-82 Satisfactory (3)
83-95 Good (4)
96-109 Excellent (5)

 

Students who pass block#A#B#C and the sum of T+L+M+B is 55 or more, but fail on the practical essay have to retake only the practical essay when they repeat the semifinal exam; students who pass the laboratory examination but fail on the written test, have to retake the blocks#A#B#C. Those who want better grade are entitled to rewrite also the block#A#B#C, however, with the risk of performing worse.

‘1 point rule’ if any of the blocks contains 14 point and the other two blocks contain 15 points or more (T) + the laboratory exam is evaluated by either 1 or 2 points (L), the grade for final-examination is 2 (pass) if the sum of scores and points (T+L+M+B) are 59 points or higher.

1Results of midterm examinations, laboratory exams of earlier studies are not accepted.

Subject matter

Subject title: Medical biochemistry, molecular and cell biology II. (Medicine) Biochemistry, molecular and cell biology II. (Dentistry)
Code: AOOBIBKM_2A (Medicine)  FOKOOBI159_2A (Dentistry)
Credit: 6 (Medicine) 5 (Dentistry)
Head of Deparment of Medical Biochemistry: Prof. Tretter, László

Description of the subject: The objective of the course is to introduce complex molecular interpretation of selected physiological processes in the human organism.

Main topics of the lectures: The subject addresses the metabolic processes in the human organism at molecular level with special emphasis on their medical aspects. The main topic is the metabolism of carbohydrates, lipids, amino acids and nucleotides and its regulation under normal and pathological conditions. Lectures cover the following topics:

  • Metabolism of compounds providing energy for the cell function.
  • Storage and mobilization of carbohydrates and lipids.
  • Gluconeogenesis and biosynthesis of sugar derivatives.
  • Biosynthesis of fatty acids, triacylglycerols, cholesterol, bile acids. Biochemistry of the steroidal hormones and the essentioal fatty acids.
  • Turn-over of proteins in the human organism.
  • Degradation of amino acids and metabolic fate of their carbon atoms.
  • Formation and elimination of ammonia. – Biosynthesis of non-essential amino acids.
  • Biosynthesis and degradation of nucleotides and heme.
  • Integration of metabolic pathways at the level of the whole body (specific metabolic properties of separate organs, metabolic communication between organs).

Students perform laboratory experiments that help them to acquire skills in basic biochemistry techniques.  Clinical biochemistry procedures and basic molecular biology techniques are practiced by the students in laboratory experiments. Problem-oriented discussions in seminars serves to introduce edge-cutting research topics of medicine (gene therapy, molecular basis of diseases with high public health impact).

  • First day of the semester: September 7, 2015
  • Last day of the semester: December 11, 2015
  • Examination period: December 12, 2015 – January 29, 2016
  • Student Affairs Secretary: Mr. Zsolt Ozsváth EOK Building, 1st Floor, Passage ‘C’; +36-1-459 1500#60061
  • Teaching secretary. Dr. István Léránt +36-1-459 1500#60034 +36-20-663 2267
  • National/Public holidays: October 23, 2015 (Friday)  Memorial day of the 1956 Hungarian Revolutions
  • No lectures /seminars: October 22 09:30- , 2015 (Thursday)

DOWNLOADS

Lecture slides – Medical Biochemistry II  (password)

Scientific articles for seminars (2015/2016) Download here (password)

Scientific articles for seminars (- 2013/2014) – DOWNLOAD HERE (user name  –  password)