Laboratory of Neuromorphology

kep-Dobolyi

Dr. Árpád Dobolyi

Head of the Laboratory: Dr. Árpád Dobolyi, PhD, research associate professor

Curriculum vitae of Árpád Dobolyi (pdf)

 

Scientific Advisor: Dr. Miklós Palkovits, MD, PhD, professor, member of the Hungarian Academy of Sciences

Members of the Laboratory:

• János Barna, PhD, research associate
• Melinda Cservenák , PhD, postdoctoral fellow
• Éva Rebeka Szabó, postgraduate PhD fellow
• Pál Gabriella, MD, postgraduate PhD fellow
• Viktória Dellaszéga-Lábas, scientific technician
• András Lékó, MD student

Pictures:

Ongoing research activity in the Laboratory of Neuromorphology:

1. Neurobiology of parenthood

  • Cservenák M., Szabó É.R., Bodnár I., Lékó A., Palkovits M., Nagy G.M., Usdin T.B., Dobolyi A. (2013) The thalamic neuropeptide TIP39 mediates the effects of nursing on lactation and maternal motivation. Psychoneuroendocrinology. 38:3070-84.
  • Varga T., Mogyoródi B., Bagó A.G., Cservenák M., Domokos D., Renner E., Gallatz K., Usdin T.B., Palkovits M., Dobolyi A. (2012) Paralemniscal TIP39 is induced in rat dams and may participate in maternal functions. Brain Struct. Funct. 217, 323–335.
  • Szabó E.R., Cservenák M., Dobolyi A. (2012) Amylin is a novel neuropeptide with potential maternal functions. FASEB J. 26, 272-281.
  • Dobolyi A. (2011) Novel potential regulators of maternal adaptations during lactation: tuberoinfundibular peptide 39 and amylin. J. Neuroendocrinol. 23, 1002-1008.
  • Cservenák M., Bodnár I., Usdin T.B., Palkovits M., Nagy G.M., Dobolyi A. (2010) Tuberoinfundibular peptide of 39 residues is activated during lactation and participates in the suckling-induced prolactin release. Endocrinology, 151, 5830-5840.
  • Dobolyi A (2009) Central amylin expression and its induction in rat dams. J. Neurochem. 111, 1490-1500.

2. Brain pathology of stroke

  • Pál G., Vincze C., Renner É., Wappler E.A., Nagy Z., Lovas G., Dobolyi A. (2012) Time course, distribution and cell types of induction of transforming growth factor betas following middle cerebral artery occlusion in the rat brain. PLoS One, 7(10):e46731.
  • Dobolyi A., Vincze C., Pál G., Lovas G. (2012) The neuroprotective functions of transforming growth factor beta proteins. Int. J. Mol. Sci. 13, 8219-8258.
  • Vincze C., Pál G., Wappler E.A., Szabó É.R., Nagy Z., Lovas G., Dobolyi A. (2010) Transforming growth factor beta isoforms in the intact rat brain and following experimentally induced focal ischemia. J. Comp. Neurol. 518, 3752-3770.

3. Neuronal function of nucleosides

  • Kovács Z., Dobolyi A., Kékesi A.K., Juhász G., (2013) 5′-nucleotidases, nucleosides, and their distribution in the brain: pathological and therapeutic implications. Curr. Med. Chem. 20:4217-4240.
  • Dobolyi A., Kovács Z., Juhasz G., Kardos J. (2011) Uridine function in the central nervous system. Curr. Top. Med. Chem. 11, 1058-1067.
  • Kovács Z., Dobolyi A., Kékesi A.K., Juhász G. (2010) Nucleoside map of the human central nervous system. Neurochem. Res. 35, 452-464.
  • Kovács Z., Dobolyi A., Juhász G., Bobest M., Papp V., Takáts L., Kékesi A.K. (2010) Gender- and age-dependent changes in nucleoside levels in the cerebral cortex and white matter of the human brain. Brain Res. Bull. 81, 579-584.

4. Pathomechanisms of epilepsy

  • Dobolyi A., Kékesi A.K., Juhász G., Székely A.D., Lovas G., Kovács Z. (2014) Receptors of peptides as therapeutic targets in epilepsy research Curr. Med. Chem. 21:764-87.
  • Györffy B., Kovács Z., Gulyássy P., Simor A., Völgyi K., Orbán G., Baracskay P., Szabó Z., Janáky T., Dobolyi A., Juhász G., Czurkó A., Kékesi A.K. (2014) Brain protein expression changes in WAG/Rij rats, a genetic rat model of absence epilepsy after peripheral lipopolysaccharide treatment. Brain Behav. Immun. 2013 Sep 8. doi:pii: S0889-1591(13)00450-9. 10.1016/j.bbi.2013.09.001. PMID: 24021561.
  • Kovács Z., Slézia A., Bali Z.K., Kovács P., Dobolyi A., Szikra T., Hernádi I., Juhász G. (2013) Uridine modulates neuronal activity and inhibits spike-wave discharges of absence epileptic Long Evans and Wistar Albino Glaxo/Rijswijk rats. Brain Res Bull. 2013 Aug;97:16-23. doi: 10.1016/j.brainresbull.2013.05.009. Epub 2013 May 23. PMID: 23707857.
  • Héja L., Nyitrai G., Kékesi O., Dobolyi A., Szabó P., Fiáth R., Ulbert I., Pál-Szenthe B., Palkovits M., Kardos J. (2012) Astrocytes convert network excitation to tonic inhibition of neurons. BMC Biol. 10, 26.

5. Neurochemistry of mitochondria

  • Dobolyi A., Ostergaard E., Bagó A.G., Dóczi T., Palkovits M., Gál A., Molnár M.J., Ádám-Vizi V., Chinopoulos C. (2014) Exclusive neuronal expression of SUCLA2 in the human brain. Brain Struct. Funct. 2013 Oct 2. PMID: 24085565.
  • Molnár T, Dobolyi A, Nyitrai G, Barabás P, Héja L, Emri Z, Palkovits M, Kardos J. (2011) Calcium signals in the nucleus accumbens: Activation of astrocytes by ATP and succinate. BMC Neurosci. 12, 96.

6. Neuronal control of food intake

  • Renner E., Puskás N., Dobolyi A., Palkovits M. (2012) Glucagon-like peptide-1 of brainstem origin activates dorsomedial hypothalamic neurons in satiated rats. Peptides 35, 14-22.
  • Renner E., Szabó-Meltzer K.I., Puskás N., Tóth Z.E, Dobolyi A., Palkovits M. (2010) Activation of neurons in the hypothalamic dorsomedial nucleus via hypothalamic projections of the nucleus of the solitary tract following refeeding of fasted rats. Eur. J. Neurosci. 31, 302-314.

 

Techniques and methods used routinely in the Laboratory:

  • Animal operations
  • Occlusion of the middle cerebral artery and other models of focal brain ischemia
  • Canulations, sampling of blood and cerebrospinal fluid in rodents
  • Behavioral tests of anxiety, depression and maternal motivation
  • Stereotaxic operations, brain lesions, pathway transections, tracer injections
  • Neuronal tract-tracing using viruses and transgenic mice
  • Laser capture microdissection and real-time RT-PCR
  • Basic molecular biological techniques
  • RNA amplification for microarray and sequencing
  • In situ hybridization histochemistry and dark-field microscopy
  • Immunolabeling and fluorescent microscopy
  • Western blotting